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1.
Am J Physiol Gastrointest Liver Physiol ; 305(5): G357-63, 2013 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-23832516

RESUMO

The mouse intestinal mucus is mainly made up by the gel-forming Muc2 mucin and the stomach surface mucus Muc5ac, both extensively O-glycosylated. The oligosaccharide diversity provides a vast library of potential recognition sites for both commensal and pathogenic organisms. The mucin glycans are thus likely very important for the selection and maintenance of a stable intestinal flora. Here we have explored the O-glycan patterns of the mouse gastrointestinal tract mucins. The mucins from the mucus of the distal and proximal colon, ileum, jejunum, duodenum, and stomach of conventionally raised wild-type (C57BL/6) mice were separated by composite gel electrophoresis. The O-linked glycans were released by reductive elimination and structurally characterized by liquid chromatography-mass spectrometry. The mucins glycans were mostly core 2 type [Galß1-3(GlcNAcß1-6)GalNAcol], but also core 1 (Galß1-3GalNAcol). In the stomach about half of the Muc5ac mucin O-glycans were neutral and many monosulfated, but with a low grade of sialylation and fucosylation. Mouse ileum, jejunum, and duodenum had similar glycan patterns dominated by sialylated and sulfated core 2 glycans, but few fucosylated. Colon was on the other hand dominated by highly charged fucosylated glycans. The distal colon is different from the proximal colon because different biosynthetic pathways are utilized, although sialylated and sulfated glycans were highly abundant in both parts. The sulfation was higher in the distal colon, whereas sialic acid was more common in the proximal colon. Many fucosylated glycans were found in both the proximal and distal colon. Thus the mucin O-glycans vary along the mouse gastrointestinal tract.


Assuntos
Colo/metabolismo , Mucosa Gástrica/metabolismo , Mucosa Intestinal/metabolismo , Intestino Delgado/metabolismo , Mucina-5AC/metabolismo , Mucina-2/metabolismo , Animais , Cromatografia Líquida , Eletroforese em Gel de Poliacrilamida , Feminino , Fucose/metabolismo , Glicosilação , Masculino , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Muco/metabolismo , Ácido N-Acetilneuramínico/metabolismo , Proteômica/métodos , Sulfatos/metabolismo
2.
Glycobiology ; 22(8): 1128-39, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22581805

RESUMO

The heavily O-glycosylated mucin MUC2 constitutes the major protein in the mucosal layer that acts as a physical barrier protecting the epithelial layer in the colon. In this study, Muc2 was purified from mucosal scrapings from the colon of wild-type (WT) mice, core 3 transferase knockout (C3Gnt(-/-)) mice and intestinal epithelial cell-specific core 1 knockout (IEC C1Galt1(-/-)) mice. The Muc2 O-glycans were released by reductive ß-elimination and analyzed with liquid chromatography-mass spectrometry in the negative-ion mode. Muc2 from the distal colon of WT and C3Gnt(-/-) knockout mice carried a mixture of core 1- or core 2-type glycans, whereas Muc2 from IEC C1Galt1(-/-) mice carried highly sialylated core 3- and core 4-type glycans. A large portion of NeuAc in all mouse models was positioned on disialylated N-acetyllactosamine units, an epitope not reported on human colonic MUC2. Mass spectra and proton NMR spectroscopy revealed an abundant NeuAc linked to internally positioned N-acetylglucosamine on colonic murine Muc2, which also differs markedly from human MUC2. Our results highlight that murine colonic Muc2 O-glycosylation is substantially different from human MUC2, which could be one explanation for the different commensal microbiota of these two species.


Assuntos
Amino Açúcares/metabolismo , Colo/metabolismo , Galactosiltransferases/fisiologia , Glicômica , Mucina-2/metabolismo , N-Acetilglucosaminiltransferases/fisiologia , Animais , Sequência de Carboidratos , Cromatografia Líquida , Epitopos , Glicosilação , Humanos , Mucosa Intestinal/citologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Espectroscopia de Ressonância Magnética , Metagenoma , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Dados de Sequência Molecular , Polissacarídeos/metabolismo , Espectrometria de Massas por Ionização por Electrospray
3.
Cell Mol Life Sci ; 68(22): 3635-41, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21947475

RESUMO

In discussions on intestinal protection, the protective capacity of mucus has not been very much considered. The progress in the last years in understanding the molecular nature of mucins, the main building blocks of mucus, has, however, changed this. The intestinal enterocytes have their apical surfaces covered by transmembrane mucins and the whole intestinal surface is further covered by mucus, built around the gel-forming mucin MUC2. The mucus of the small intestine has only one layer, whereas the large intestine has a two-layered mucus where the inner, attached layer has a protective function for the intestine, as it is impermeable to the luminal bacteria.


Assuntos
Mucosa Intestinal/anatomia & histologia , Mucosa Intestinal/química , Mucosa Intestinal/metabolismo , Animais , Enterócitos/química , Enterócitos/citologia , Enterócitos/metabolismo , Humanos , Imunidade nas Mucosas/imunologia , Mucosa Intestinal/microbiologia , Intestinos/anatomia & histologia , Intestinos/microbiologia , Intestinos/fisiologia , Modelos Moleculares , Mucinas/química , Mucinas/metabolismo
4.
Anal Chem ; 82(4): 1470-7, 2010 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-20092260

RESUMO

Negative ion mode nanoliquid chromatography/mass spectrometry (nano-LC/MS) on porous graphitic carbon columns at pH 11 was studied and compared to capillary LC/MS at pH 8 for the analysis of neutral and acidic glycan alditols. Oligosaccharides were chromatographed with an acetonitrile gradient containing 0.04% ammonium hydroxide and analyzed with a linear ion trap mass spectrometer (LTQ) equipped with a modified nanospray interface. Analysis of acidic N- and O-glycan standards revealed that good quality MS/MS spectra could be obtained when loading 1-3 fmol, a 10-fold increase in sensitivity compared to capillary-LC/MS at pH 8. Analysis of a complex mixture of O-glycans from porcine colonic mucins with nano-LC/MS and MS/MS at high pH revealed 170 oligosaccharides in one analysis, predominantly corresponding to sulfated glycans with up to 11 residues. Analysis of the same sample with capillary-LC/MS showed a lower sensitivity for multiply sulfated glycans. Nano-LC/MS of O-linked oligosaccharides on MUC2 from a human colon biopsy also illustrated that the ionization of oligosaccharides with multiple sialic acid groups was increased compared to those with only one sialic acid residue. Nano-LC/MS at high pH is, thus, a highly sensitive approach for the analysis of acidic oligosaccharides.


Assuntos
Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Ácido N-Acetilneuramínico/química , Nanotecnologia , Polissacarídeos/análise , Polissacarídeos/química , Ácidos Sulfúricos/química , Animais , Sequência de Carboidratos , Cromatografia Líquida/normas , Humanos , Concentração de Íons de Hidrogênio , Espectrometria de Massas/normas , Dados de Sequência Molecular , Mucinas/química , Nitrogênio/química , Oligossacarídeos/análise , Oligossacarídeos/química , Oxigênio/química , Padrões de Referência
5.
Glycoconj J ; 26(9): 1125-34, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19326211

RESUMO

Cervical mucins are glycosylated proteins that form a protective cervical mucus. To understand the role of mucin glycans in Candida albicans infection, oligosaccharides from mouse cervical mucins were analyzed by liquid chromatography-mass spectrometry. Cervical mucins carry multiple alpha(1-2)fucosylated glycans, but alpha(1,2)fucosyltransferase Fut2-null mice are devoid of these epitopes. Epithelial cells in vaginal lavages from Fut2-null mice lacked Ulex europaeus agglutinin-1 (UEA-I) staining for alpha(1-2)fucosylated glycans. Hysterectomy to remove cervical mucus eliminated UEA-I and acid mucin staining in vaginal epithelial cells from wild type mice indicating the cervix as the source of UEA-I positive epithelial cells. To assess binding of alpha(1-2) fucosylated glycans on C. albicans infection, an in vitro adhesion assay was performed with vaginal epithelial cells from wild type and Fut2-null mice. Vaginal epithelial cells from Fut2-null mice were found to bind increased numbers of C. albicans compared to vaginal epithelial cells obtained from wild type mice. Hysterectomy lessened the difference between Fut2-null and wild type mice in binding of C. ablicans in vitro and susceptibility to experimental C. albicans vaginitis in vivo. We generated a recombinant fucosylated MUC1 glycanpolymer to test whether the relative protection of wild type mice compared to Fut2-null mice could be mimicked with exogenous mucin. While a small portion of the recombinant MUC1 epitopes displayed alpha(1-2)fucosylated glycans, the predominant epitopes were sialylated due to endogenous sialyltransferases in the cultured cells. Intravaginal instillation of recombinant MUC1 glycanpolymer partially reduced experimental yeast vaginitis suggesting that a large glycanpolymer, with different glycan epitopes, may affect fungal burden.


Assuntos
Candidíase Vulvovaginal/prevenção & controle , Muco do Colo Uterino/metabolismo , Fucose/metabolismo , Mucinas/metabolismo , Polissacarídeos/metabolismo , Animais , Candida albicans/crescimento & desenvolvimento , Candidíase Vulvovaginal/induzido quimicamente , Candidíase Vulvovaginal/patologia , Sequência de Carboidratos , Adesão Celular , Muco do Colo Uterino/microbiologia , Contagem de Colônia Microbiana , Suscetibilidade a Doenças , Células Epiteliais/enzimologia , Células Epiteliais/microbiologia , Células Epiteliais/patologia , Epitopos/imunologia , Feminino , Fucosiltransferases/deficiência , Fucosiltransferases/metabolismo , Histerectomia , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Mucina-1/metabolismo , Proteínas Recombinantes/metabolismo , Esfregaço Vaginal , Galactosídeo 2-alfa-L-Fucosiltransferase
6.
Mol Cell Proteomics ; 6(4): 708-16, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17220477

RESUMO

The mucus filling the human cervical opening blocks the entry to the uterus, but this has to be relative and allow for the sperm to penetrate at ovulation. We studied this mucus, its content of proteins and mucins, and the mucin O-glycosylation in cervical secretions before, during, and after ovulation. Cervical mucosal secretions from 12 subjects were collected, reduced-alkylated, separated with polyacrylamide or agarose/polyacrylamide gel electrophoresis, and stained with silver, Alcian blue, or Coomassie Blue stain. Protein and mucin bands from before and during ovulation were digested and subsequently analyzed by nano-LC-FT-ICR MS and MS/MS. We identified 194 proteins after searches against the NCBI non-redundant protein database and an in-house mucin database. Three gel-forming (MUC5B, MUC5AC, and MUC6) and two transmembrane mucins (MUC16 and MUC1) were identified. For the analysis of mucin O-glycosylation, separated mucins from six individuals were blotted to PVDF membranes, and the O-glycans were released by reductive beta-elimination and analyzed with capillary HPLC-MS and -MS/MS. At least 50 neutral, sialic acid-, and sulfate-containing oligosaccharides were found. An increase of GlcNAc-6GalNAcol Core 2 structures and a relative decrease of NeuAc residues are typical for ovulation, and NeuAc-6GalNAcol and NeuAc-3Gal- epitopes are typical for the non-ovulatory phases. The cervical mucus at ovulation is thus characterized by a relative increase in neutral fucosylated oligosaccharides. This comprehensive characterization of the mucus during the menstrual cycle suggests mucin glycosylation as the major alteration at ovulation, but the relation to the altered physicochemical properties and sperm penetrability is still not understood.


Assuntos
Muco do Colo Uterino/química , Ciclo Menstrual/metabolismo , Mucinas/química , Proteínas/química , Adulto , Sequência de Carboidratos , Feminino , Glicosilação , Humanos , Dados de Sequência Molecular , Mucinas/isolamento & purificação , Proteínas/isolamento & purificação , Proteômica , Espectrometria de Massas em Tandem
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