RESUMO
OBJECTIVE: We evaluated whether the proteasomal chymotrypsin-like (ChT-L) activity is increased in plasma of patients with acute lymphoblastic (ALL), acute myeloblastic (AML) and chronic lymphocytic (CLL) leukemias. METHODS: The activity was assayed using the fluorogenic peptide substrate in the presence of an artificial activator sodium dodecyl sulfate (SDS) in the plasma of healthy donors (n=15) and ALL (n=15), AML (n=28) and CLL (n=22) patients. RESULTS: The activity was significantly (P<0.001) higher in the plasma of ALL and AML patients at the diagnosis than in healthy subjects and decreased after therapy or remained unchanged or rose during relapse. By contrast, in CLL patients at the diagnosis, the activity did not differ significantly from the healthy controls. In each group, the activity positively correlated with the serum lactic dehydrogenase activity. CONCLUSIONS: Plasma proteasome ChT-L activity can be a useful bio-marker for patients with acute leukemia at the blast stage.
Assuntos
Quimotripsina/sangue , Leucemia/sangue , Complexo de Endopeptidases do Proteassoma/sangue , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Hidrólise/efeitos dos fármacos , L-Lactato Desidrogenase/sangue , Leucemia/diagnóstico , Leucemia Linfocítica Crônica de Células B/sangue , Leucemia Linfocítica Crônica de Células B/diagnóstico , Leucemia Mieloide Aguda/sangue , Leucemia Mieloide Aguda/diagnóstico , Masculino , Pessoa de Meia-Idade , Oligopeptídeos/farmacologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/sangue , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Inibidores de Proteassoma , Subunidades Proteicas/metabolismo , Dodecilsulfato de Sódio/farmacologiaRESUMO
UNLABELLED: The aim of the study was the evaluation of number and morphologic parameters of blood platelets and sodium-proton platelet exchangers in pregnant women in the 3rd trimester of pregnancy, healthy (155) and with preeclampsia (40). RESULTS: Blood platelet number was significantly lower in patients with preeclampsia than in healthy subjects (195,000 vs 222,000). Mean platelet volume was significantly higher in patients with preeclampsia (9.5 fl vs 8.6 fl). The activity of Na+/H+ was slightly higher in pregnants with preeclampsia.
Assuntos
Plaquetas/citologia , Pré-Eclâmpsia/sangue , Gravidez/metabolismo , Trocadores de Sódio-Hidrogênio/metabolismo , Adulto , Plaquetas/metabolismo , Plaquetas/patologia , Estudos de Casos e Controles , Feminino , Humanos , Hipertensão/sangue , Recém-Nascido , Valor Preditivo dos Testes , Gravidez/sangue , Complicações Cardiovasculares na Gravidez/sangue , Terceiro Trimestre da Gravidez , Fatores de Risco , Estatísticas não ParamétricasRESUMO
Our purpose was to investigate the amniotic fluid lamellar body count and interleukin-6 levels in women with preterm labor. The study was carried out on 50 amniotic fluid collections obtained by amniocentesis or during cesarean delivery. Concentration of lamellar body (CL) and interleukin-6 (IL-6) were performed. We found significantly lower lamellar body count values in women at risk of preterm delivery before 34 weeks' gestation and elevated amniotic fluid IL-6 concentration (CL--20.81 +/- 6.73 x 103/ul, IL-6--318.3 +/- 18.7 pg/ml) when compared with pregnancies at term (CL--77.1 +/- 28 x 103/ul, IL-6--142 +/- 15.3 pg/ml).
