1.
Chem Asian J
; 9(7): 1786-96, 2014 Jul.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24799439
RESUMO
Small glycodendrimers with α-mannosyl ligands were synthesized by using copper-catalyzed azide-alkyne coupling chemistry and some of these molecules were used as multivalent ligands to study the induction of concanavalinâ A (Conâ A) precipitation. The results showed that the monovalent mannose ligand could induce the precipitation of Conâ A. This unexpected finding initiated a series of studies to characterize the molecular basis of the ligand-lectin interaction. The atypical precipitation is found to be specific to the mannose, fluorescein moiety (FITC), and Conâ A. Apparently the mannose ligand binds to Conâ A through hydrogen-bonding interactions, whereas the binding of FITC is mediated by hydrophobic forces.