RESUMO
Eight new geranylated and farnesylated pyranoflavanones (1-8) and two new farnesylated pyranochalcones (9 and 10) were isolated from the methanolic extract of the fruits of Amomum tsao-ko. Their structures were elucidated by extensive analysis of the 1D and 2D NMR spectra and from the HRESIMS, combined with experimental ECD data. All compounds were evaluated for their inhibitory effects on the nitric oxide production against lipopolysaccharide-induced RAW 264.7 macrophages and exhibited IC50 values ranging from 10.6 to 41.5 µM.
Assuntos
Amomum/química , Chalconas/isolamento & purificação , Flavanonas/isolamento & purificação , Piranos/química , Animais , Chalconas/química , Flavanonas/química , Camundongos , Estrutura Molecular , Células RAW 264.7 , Análise Espectral/métodosRESUMO
Two new sesquiterpenoids (1 and 2) and a new ent-pimarane type diterpenoid (3), together with eighteen known compounds (4-21), were isolated from the whole plants of Siegesbeckia pubescens. The structures of the new compounds were determined on the basis of 1D-, 2D NMR and HRESIMS data. All compounds were evaluated for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Of these, highly oxygenated germacrane type sesquiterpenoids (1-2 and 13-14) showed significant inhibitory effects with IC50 values ranging from 3.9 to 16.8⯵M.
