Ectopic vascularized bone formation by human umbilical cord-derived mesenchymal stromal cells expressing bone morphogenetic factor-2 and endothelial cells.

Mesenchymal stromal cells (MSCs) isolated from numerous tissues including human fetal tissue are currently used in cell therapy and regenerative medicine. Among fetal tissues, the umbilical cord (UC) is one of the sources for both MSCs and endothelial cells (ECs). To establish ectopic vascularized bone tissue formation, UC-derived MSCs and ECs were isolated. UC-MSCs expressing human BMP-2 (hBMP-2-MSCs) were generated using an adenoviral system to promote bone formation. These cells were then transplanted with Matrigel into the subcutaneous tissue of an immune deficient NSG mouse, and bone tissue was analyzed after several weeks. The osteogenic differentiation ability of MSCs was elevated by transduction of the hBMP-2 expressing adenoviral system, and vascularization of bone tissue was enhanced by human umbilical vein endothelial cells (HUVEC). In this study, our results provide evidence that MSCs and HUVECs from human umbilical cord are suitable cells to investigate bone tissue engineering. The results also suggest that the co-transplantation of hBMP2-MSCs and HUVECs may be a simple and efficient strategy for improving tissue generation and angiogenesis in bone tissue engineering using stem cells.

The Normal Value of Adult Korean Orbital Volume in Three-Dimensional Computerized Tomography

PURPOSE: This study is to know a normal orbital volume of adult Koreans. METHODS: We had taken 3-dimension orbital CT of 100 patients who had visited the department of ophthalmology and emergency room. Among them 30 patients who were reported to show abnormal orbital CT finding were excluded. Thereafter we measured orbital CT volume in eyes of 70 patients. We appointed inner sides of orbital wall in coronal plane image and reconstructed 3-dimension images by means of soft ware program(silhouette) in CT to measure the orbital volume. RESULTS: Average of orbital volume was 24.5 mm 3(left orbital vol. 24.25 mm 3, right 24.75 mm 3), in males and 23.5 mm3(left 23.25 mm 3 , right 23.75 mm 3)in females. Difference between right orbit and left orbit was 2% and average of male orbital volume was bigger than female by 4%. CONCLUSION: Adult Korean orbital volume measure was useful for differential diagnosis in orbital desease.

Two Cases of Acute Acquired Comitant Esotropia

Acute acquired comitant esotropia has been used to describe a dramatic onset of a relatively large angle of esotropia with diplopia and minimal refractive errors. The authors experienced two cases of acute acquired comitant esotropia in patients with the age of 28 and 40 years. Sudden diplopia and esodeviation developed in both patients. All ophthalmologic and neurologic examinations including brain MRI showed negative results. Amount of deviation remained constant. We performed recession of both medial rectus muscles. Four weeks after surgery, both patients showed orthotropia without any complaints of diplopia.

An Anatomical Study on Transscleral Suture fixation of Posterior Chamber Intraocular Lens

We performed an anatomical study on transscleral suture fixation of posterior chamber intraocular lens implantation. Results showed that the relationship of the ciliary sulcus to the overlying posterior surgical limbus differed in the vertical and horizontal meridians. Needles that pierced the ciliary sulcus after being passed perpendicularly through the sclera entered the sclera 0.88+/-0.22mm posterior to the posterior surgical limbus in the vertical meridians and 0.44+/-0.37mm in the horizontal meridians. The scleral exit site of a suture passed from the inside of the eye through ciliary sulcus was 0.90+/-0.12mm posterior to the posterior surgical limbus in the vertical meridians, 0.85+/-0.17mm in the oblique meridians, and 0.45+/-0.10mm in the horizontal meridian.

An Epidemiologic Survey of Strabismus among School-Children in Korea

We Performed an epidemiologic survey for strabismus among primary school-children in order to investigate the number of strabismus from March 1995 to February 1996. Cover-uncover test and prism cover test were used. The results were as follows:1. Out of 9054 1st grade primary school-children examined, 322(3.56%) were found as strabismus. 2. 322 cases of strabismus include 262(81.4%)exodeviation and 60(18.6%) esodeviation. 3. Alternating type and monocular type strabismus have no difference both in exotropia and esotrapia. 4. In exotropia and esotropia, no sexual predilection was found. 5. Most portion of deviation angle distribute between 11 to 20 prism diopter. 6. The difference of number of strabismus between big cities and small cities was statistically insignificant(p>0.05).