User experience of and satisfaction with computer-aided design software when designing dental prostheses: A multicenter survey study.

AIM: The current study aimed to compare the responses and satisfaction reported by users with varying levels of experience when using different types of computer-aided design (CAD) software programs to design crowns. MATERIALS AND METHODS: A questionnaire was used to evaluate user responses to five domains (software visibility, 3Dscanned data preparation, crown design and adjustment, finish line registration, and overall experience) of various CAD software programs. The study included 50 undergraduate dental students (inexperienced group) and 50 dentists or dental technicians from two hospitals (experienced group). The participants used four different CAD software programs (Meshmixer, Exocad, BlueSkyPlan, and Dentbird) to design crowns and recorded the features using the questionnaire. Statistical analyses included one-way and two-way analysis of variance (ANOVA) tests to compare scores and verify the interaction between user response and experience. RESULT: User evaluation scores in the domains of software visibility and 3D-scanned data preparation varied between software programs (P < 0.001), with Exocad being favored by the experienced group. When evaluating crown design and finish line registration, Dentbird and Exocad scored significantly higher than the other software in both groups as they offered automation of the process using deep learning (P < 0.001). Two-way ANOVA showed that prior experience of using CAD significantly affected the users' responses to all queries (P < 0.001). CONCLUSION: User response and satisfaction varied with the type of CAD software used to design dental prostheses, with prior experience of using CAD playing a significant role. Automation of design functions can enhance user satisfaction with the software.

Influence of limited mouth opening in children on intraoral scanning accuracy: An in vitro study.

BACKGROUND: Although intraoral scanning is highly reliable, little is known about its accuracy in young children with limited mouth-opening ability. AIM: To determine the accuracy of intraoral scans based on the degree of mouth opening. DESIGN: To simulate mouth opening in children with primary dentition, three groups (n = 5 per group) were allocated by maximum mouth opening of 30, 37 and 40 mm. After the primary dentition model was connected to a dental phantom, intraoral scanning was performed using iTero and TRIOS4. The scanned files were digitally evaluated. Root mean square values were calculated to assess trueness and precision. RESULTS: iTero showed deviations of three-dimensional trueness of 0.067 ± 0.008, 0.063 ± 0.001 and 0.065 ± 0.005 mm, and TRIOS4 of 0.07 ± 0.002, 0.064 ± 0.003 and 0.066 ± 0.002 mm in the 30, 37 and 40 mm groups, respectively. There were no significant differences in either mouth opening (p > .017) or the intraoral scanners (p > .05). The same statistical results were obtained for precision, with the exception of the 30 mm of mouth opening. CONCLUSIONS: Within the limits of this study, limited mouth opening hardly influenced the accuracy of intraoral scanning.

Accuracy of impression methods through the comparison of 3D deviation between implant fixtures.

AIM: To compare the accuracy of three impression methods by comparing the distance between the reference points of the implant fixture, especially in curved maxillary anterior teeth. MATERIALS AND METHODS: Implant fixtures were placed in the maxillary central incisor and canine regions. A maxillary master cast was made using a model scanner and 3D printer. Ten impressions were taken from the three experimental groups constructed (group P: pick-up impression coping; group I: scan body with an intraoral scanner; group B: bite impression coping). The distance between the reference points, the angle between the scan bodies, and displacement of the 3D surface area were measured. RESULTS: The distances between the reference points were significantly different between groups I and B in the maxillary incisors, and between group P and the other two groups in the maxillary canines. Group P had the least amount of displacement in both fixtures. Both fixtures showed the highest displacement in group B. Displacement of the 3D surface area in the maxillary incisors showed no significant difference between the groups. There was a significant difference in the maxillary canines between groups P and I. CONCLUSIONS: In the present study, all three implant impression methods showed changes in the position and angle of the fixture compared with the master cast. The highest accuracy was shown by the impression method using the pick-up impression coping, but the impression method using the intraoral scanner also showed clinically acceptable accuracy. It should be noted that errors may occur when taking impressions using a bite impression coping.

Risk factors associated with screw loosening in CAD-CAM custom abutments: A 6-year retrospective study.

STATEMENT OF PROBLEM: Custom abutments made by using computer-aided design and computer-aided manufacturing (CAD-CAM) are often used for implant-supported prostheses. However, studies on screw loosening of implant prostheses using custom abutments are lacking. PURPOSE: The purpose of this retrospective study was to investigate the risk factors of screw loosening in implant-supported prostheses using custom abutments made with CAD-CAM. MATERIAL AND METHODS: Clinical charts, radiographic images, and CAD custom abutment design file data were collected and analyzed retrospectively. The study included 255 patients with 401 dental implants, and the follow-up duration was 6 years. Age, sex (male/female), type of unit (single-unit or multiunit), location of the implant (maxilla or mandible and anterior, premolar, or molar tooth), type of antagonist, and experience of the prosthodontist were reviewed, and implant angulation (mesiodistal and buccolingual), location of the screw access hole (mesiodistal and buccolingual distances from center), and attrition status (none, localized, or generalized) were measured by using a CAD file. The frequencies and percentages of risk factors were evaluated with the Fisher exact test, and the Bonferroni correction was used as a post hoc test. Multiple logistic regression with the Firth method was performed to calculate the odds ratios and their confidence intervals (α=.05). RESULTS: Screw loosening showed a correlation with the severity of attrition (P<.001). Single-unit prostheses had a higher risk of screw loosening than multiunit prostheses (P<.001). Experience of the prosthodontist (P<.001). Buccolingual angulation of the implant and abutment significantly influenced the incidence of screw loosening (P<.05). Age, sex, location of the implant, type of antagonist, mesiodistal implant angulation, and buccolingual and mesiodistal location of the screw access hole did not significantly influence the incidence of screw loosening (P>.05). CONCLUSIONS: The degree of attrition, buccolingual angulation of the implant and abutment, type of unit, and experience of the prosthodontist (>3 years or <3 years) were risk factors in the incidence of screw loosening.

Assessment of the fit of lithium disilicate crowns at various locations fabricated by three different methods using the triple-scan protocol.

AIM: The present study aimed to evaluate the marginal and internal fit of lithium disilicate crowns at various locations. MATERIALS AND METHODS: A typodont maxillary left first molar was prepared for a lithium disilicate crown, scanned, and a master die fabricated. Three groups were created according to fabrication method (n = 10): conventional impression and press method (group C); scanning of definitive cast and milling method (group D); and intraoral scanning and milling method (group I). Assessment was performed using the triple-scan protocol. At the buccopalatal and mesiodistal sections, the absolute marginal discrepancy (AMD), marginal gap (MG), axial internal gap (AI), and line angle internal gap (LI) were measured. One-way analysis of variance (ANOVA) and post hoc Tukey HSD tests were used for statistical analysis (α = 0.05). RESULTS: AMD values were significantly lower in group C than in groups D and I (P < 0.05). MG values in group C were significantly lower than those in the buccal and distal areas in group D and all areas in group I (P < 0.05). AI values in the buccal and palatal areas in group D were significantly lower than those in the mesial and distal areas in group D and all areas in groups C and I (P < 0.05). LI values were significantly lower in group C than in groups D and I (P < 0.05). CONCLUSION: All three methods were clinically acceptable except for the marginal fit of the intraoral scanning and milling method, which was on the borderline of a clinically acceptable fit. (Int J Comput Dent 2023;26(1): 37-0; doi: 10.3290/j.ijcd.b3818305).

Evaluation of the intaglio surface trueness and fit of zirconia crowns fabricated using different machining strategies with a chairside CAD/CAM system.

AIM: This study aimed to evaluate the intaglio surface trueness and fit of zirconia crowns depending on the different machining strategies used with the CEREC system. MATERIALS AND METHODS: Thirty duplicate tooth models for a single zirconia crown were randomly assigned to the three groups (n = 10) according to the machining mode used for fabrication: grinding, wet milling, and dry milling. The scan data of the final crowns were compared to their design data to evaluate the intaglio surface trueness. The marginal and internal fits were evaluated using the cross-sectional method. The time required for the machining and sintering processes was measured for each group. RESULT: The wet milling group showed better trueness (RMS, 13.8 ± 1.0 µm) than the grinding and dry milling groups (p < 0.001). The marginal gap was greater in the grinding group (58.6 ± 28.9 µm) than that in the wet milling and dry milling groups (p < 0.001). The dry milling group required the shortest time for the manufacturing process. CONCLUSION: All machining modes fabricated crowns with a clinically acceptable trueness and fit. However, the dry milling mode was advantageous for the chairside CAD/CAM system with respect to time efficiency.

Retention force and stress distribution analysis of the cementless double crown-type implant-supported prosthesis.

STATEMENT OF PROBLEM: Implant-supported fixed dental prostheses have been classified into screw-retained and cement-retained types, and each retaining type has complications. A novel retentive cementless double crown (CLD) type of the implant-supported fixed prosthesis has been developed. CLD has air pockets in the intaglio surface of the crown and does not require cement or a screw hole. However, studies on the retention force and stress distribution of the system are lacking. PURPOSE: The purpose of this in vitro study was to evaluate the retention force and stress distribution in the CLD system. MATERIAL AND METHODS: The specimen comprised an implant, a titanium abutment, and a zirconia crown. Retention forces of 10 specimens of the CLD type were measured at no loading and after cyclic loading for 50, 100, 200, 600, 10 000, and 1 000 000 cycles by using a universal testing machine with a custom attachment device. Forty specimens of the stress distribution test were divided into 4 groups based on the retention type (cement-retained or CLD type) and load direction (vertical or oblique). Strain gauges were attached onto the buccal and lingual sides of the implant, and microstrain values were measured. One-way analysis of variance with the post hoc Tukey honestly significant difference test was performed on the retention force data, and the t test was performed to analyze the microstrain value data (α=.05). RESULTS: Retention forces after 0, 50, 100, 200, 600, 10 000, and 1 000 000 load cycles were 18.12 ±6.16 N, 20.47 ±5.78 N, 19.79 ±6.61 N, 18.46 ±5.23 N, 19.60 ±6.93 N, 21.75 ±5.03 N, and 40.91 ±9.32 N, respectively, and after 1 000 000 cycles, the retention force was significantly higher than that of other load cycles (P<.05). The mean of maximum microstrain values under the vertical load were similar in the cement-retained type (buccal side, 834.96 ±53.69 µm/m; lingual side, 490.76 ±34.12 µm/m) and the CLD type (buccal side, 814.28 ±71.20 µm/m; lingual side, 479.10 ±30.74) (P>.05), and the mean of maximum microstrain values under the oblique load was also similar in the cement-retained type (buccal side, 1991.04 ±109.89 µm/m; lingual side, -2232.41 ±189.88) and the CLD type (buccal side, 1932.47 ±152.51 µm/m; lingual side, -2097.47 ±130.69 µm/m) (P>.05). CONCLUSIONS: The CLD type had clinically acceptable retention during 1 000 000 load cycles and had a similar or better stress distribution capability than the cement-retained type.

Influence of adjacent teeth on the accuracy of intraoral scanning systems for class II inlay preparation.

OBJECTIVE: This study aimed to evaluate the influence of the presence of adjacent teeth on the accuracy of intraoral scanning (IOS) systems for class II inlay preparation. MATERIALS AND METHODS: The mesio-occlusal inlay preparation was prepared in an anatomical model of the maxillary molar. The prepared tooth was secured to a typodont with the mesial adjacent tooth removed or in situ. Ten digital impressions of the inlay preparation were acquired using three IOS systems (CEREC Primescan, 3Shape TRIOS 3, and Medit i500). A laboratory scanner (3Shape E3) was used to obtain the reference scan data. The mean absolute deviation values were calculated to evaluate the accuracy of the digital models. RESULTS: The group with the adjacent teeth present showed lower trueness and precision compared to that without the adjacent tooth (p < .05). Significant differences were observed among the IOS systems (p < .05). Primescan showed the highest accuracy, irrespective of the presence of adjacent teeth. CONCLUSIONS: The presence of the adjacent tooth negatively affected the accuracy of all the IOSs tested. Although the performance of Primescan was superior to that of TRIOS 3 and i500, each IOS system showed clinically acceptable levels of accuracy for class II inlay preparation. CLINICAL SIGNIFICANCE: The adjacent tooth can be a confounding factor for accurate digital impressions of class II inlay preparation.

A Digital Fabrication of Dental Prosthesis for Preventing Self-Injurious Behavior Related to Autism Spectrum Disorder: A Case Report.

This case report aimed to demonstrate the prosthetic solution of an autism patient with self-injurious behavior using digital dentistry. A 24-year-old male visited our clinic with chief complaints of severe gingival recession associated with self-injurious behavior. Bilateral fixed prosthesis with denture flange were delivered using a digital workflow for the protection of the gingiva. The patient showed healed gingival tissue, behavioral modification, and acceptable oral hygiene during the follow-up period. Also, his caregivers reported no recurrence of the self-injurious behavior. Autism patients usually show self-injurious behavior, which can damage their oral tissue. With adoption of this prosthesis, behavior modification as well as healing of oral tissue was achieved.

Setting the sagittal condylar inclination on a virtual articulator by using a facial and intraoral scan of the protrusive interocclusal position: A dental technique.

Setting the condylar inclination of a dental articulator enables the delivery of prostheses without occlusal interferences. This technique article describes obtaining the sagittal condylar inclination (SCI) by using a facial and an intraoral scan of the protrusive interocclusal position. The SCI can be used on a virtual articulator in dental computer-aided design (CAD) software programs, which can facilitate the fabrication of functional prostheses without occlusal interferences.

Biomechanical stress and microgap analysis of bone-level and tissue-level implant abutment structure according to the five different directions of occlusal loads.

PURPOSE: The stress distribution and microgap formation on an implant abutment structure was evaluated to determine the relationship between the direction of the load and the stress value. MATERIALS AND METHODS: Two types of three-dimensional models for the mandibular first molar were designed: bone-level implant and tissue-level implant. Each group consisted of an implant, surrounding bone, abutment, screw, and crown. Static finite element analysis was simulated through 200 N of occlusal load and preload at five different load directions: 0, 15, 30, 45, and 60°. The von Mises stress of the abutment and implant was evaluated. Microgap formation on the implant-abutment interface was also analyzed. RESULTS: The stress values in the implant were as follows: 525, 322, 561, 778, and 1150 MPa in a bone level implant, and 254, 182, 259, 364, and 436 MPa in a tissue level implant at a load direction of 0, 15, 30, 45, and 60°, respectively. For microgap formation between the implant and abutment interface, three to seven-micron gaps were observed in the bone level implant under a load at 45 and 60°. In contrast, a three-micron gap was observed in the tissue level implant under a load at only 60°. CONCLUSION: The mean stress of bone-level implant showed 2.2 times higher than that of tissue-level implant. When considering the loading point of occlusal surface and the direction of load, higher stress was noted when the vector was from the center of rotation in the implant prostheses.

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA.

This study aimed to analyze the effects of pulp capping materials on gene expression changes in primary tooth-derived dental pulp cells using next-generation sequencing. Dental pulp cells were extracted and treated with mineral trioxide aggregate (MTA), Biodentine (BD), or TheraCal LC (TC). Cell viability assays were performed. Total RNA was extracted and analyzed through mRNA sequencing. Bioinformatic analysis of differential gene expression in dental pulp cells exposed to BD or TC versus MTA was performed. MTA, BD, and TC exposure had no significant effect on pulp cell viability (p > 0.05). Gene sets associated with inflammatory response (p = 2.94 × 10-5) and tumor necrosis factor alpha (TNF-α) signaling via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway (p = 2.94 × 10-5) were enriched in all materials. In BD-treated cells, Wnt/ß-catenin signaling (p = 3.15 × 10-4) gene sets were enriched, whereas enrichment of interferon gamma (IFN-γ) response (p = 3 × 10-3) was observed in TC-treated cells. In gene plot analysis, marked increases in receptor activator of nuclear factor kappa-Β ligand (RANKL) expression were seen in TC-treated cells over time. Despite the similar cell viabilities exhibited among MTA-, BD-, and TC-treated cells, patterns of gene networks differed, suggesting that diverse functional gene differences may be associated with treatment using these materials.

Three-Dimensional Evaluation of Peri-implant Soft Tissue When Tapered Implants Are Placed: Pilot Study with Implants Placed Immediately or Early Following Tooth Extraction.

PURPOSE: This study examined a new 3D volumetric analysis method for the assessment of baseline-to-12-month changes of the soft tissue volume at early and immediately placed tapered implants after loading with ceramic single crowns. MATERIALS AND METHODS: Eligible patients with one incisor, canine, or premolar to be extracted were included. The patients were divided randomly into early-placement or immediate-placement groups. Tapered implants (BLT, Institut Straumann) were placed after the extractions. In the early-placement group, the implants were placed 8 weeks after extraction. In the immediate-placement group, the implants were placed immediately after the extraction. All implants healed transmucosally, and the final crowns were inserted after healing (baseline). Impressions were made at screening, baseline, and 12 months after crown insertion (Permadyne, 3M). The casts were scanned (Imetric 4D) and aligned, and a superimposed area of interest (AOI) (labial/buccal aspects) was defined to assess the volumetric changes (GOM Inspect). Specific software (3Matic, Materialise NV) was used for volumetric analysis. The vertical mucosal recession was measured at each time point. Repeated-measures one-way analysis of variance and the Tukey method were used for statistical analysis (SPSS 22, IBM). RESULTS: Twenty tapered implants (16 regular and four narrow) were placed in 20 patients (12 men and 8 women) in the early-placement (EP; n = 10) and immediate-placement (IP; n = 10) groups, respectively. Threedimensional volumetric analysis revealed soft tissue volume loss in both groups of 10.0 ± 16.5 mm3 (EP) and 24.3 ± 21.3 mm3 (IP) between baseline and 12 months (P = .6). The analysis also revealed local differences in the changes, displaying both localized gain and loss in both groups. CONCLUSION: With this novel 3D analysis method, true volumetric soft tissue differences, ie, both localized gain and loss, were specified between the treatment groups.

A novel retentive type of dental implant prosthesis: marginal fitness of the cementless double crown type implant prosthesis evaluated by bacterial penetration and viability.

PURPOSE: This study aims to compare the marginal fitness of two types of implant-supported fixed dental prosthesis, i.e., cementless fixation (CL.F) system and cement-retained type. MATERIALS AND METHODS: In each group, ten specimens were assessed. Each specimen comprised implant lab analog, titanium abutment fabricated with a 2-degree tapered axial wall, and zirconia crown. The crown of the CL.F system was retained by frictional force between abutment and relined composite resin. In the cement-retained type, zinc oxide eugenol cement was used to set crown and abutment. All specimens were sterilized with ethylene oxide, immersed in Prevotella intermedia culture in a 50 mL tube, and incubated with rotation. After 48 h, the specimens were washed thoroughly before separating the crown and abutment. The bacteria that penetrated into the crown-abutment interface were collected by washing with 500 µL of sterile saline. The bacterial cell number was quantified using the agar plate count technique. The BacTiter-Glo Microbial Cell Viability Assay Kit was used to measure bacterial adenosine triphosphate (ATP)-bioluminescence, which reflects the bacterial viability. The t-test was performed, and the significance level was set at 5%. RESULTS: The number of penetrating bacterial cells assessed by colony-forming units was approximately 33% lower in the CL.F system than in the cement-retained type (P<.05). ATP-bioluminescence was approximately 41% lower in the CL.F system than in the cement-retained type (P<.05). CONCLUSION: The CL.F system is more resistant to bacterial penetration into the abutment-crown interface than the cement-retained type, thereby indicating a precise marginal fit.

Feasibility of 3-Dimensional Visual Guides for Preparing Pediatric Zirconia Crowns: An In Vitro Study.

This study evaluated the feasibility of a tooth preparation guide for prefabricated zirconia crowns (PZCs). Three-dimensional surface data for PZCs of the left maxillary primary first molar and left mandibular primary second molar were obtained using a model scanner. The tooth preparation data were digitally designed to harmonize with the adjacent teeth on the mixed dentition model and visualized using a color-coded map, which presents the required amount of tooth reduction. Twenty participants were recruited for preparing teeth with and without using the tooth preparation guide. The following three parameters were evaluated: tooth preparation time, harmony score, and amount of tooth reduction. The preparation time when using the guide was significantly reduced (p < 0.05), and a significant difference was observed in the harmony scores for the maxillary primary first molar preparation. Furthermore, the amount of tooth reduction was significantly different for both maxillary and mandibular primary molars (p < 0.05) in terms of the occlusal distal surface and buccal line angle in the maxillary primary first molars, and the smooth surfaces, proximal surfaces, and mesial line angles in the mandibular primary second molars. Thus, the results suggest that a tooth preparation guide could facilitate better tooth preparation for PZCs.

Axial Triangle of the Maxillary Sinus, and its Surgical Implication With the Position of Maxillary Sinus Septa During Sinus Floor Elevation: A CBCT Analysis.

The aim of this study was to measure the convexity of the lateral wall of the maxillary (Mx) sinus and identify the locational distribution of antral septa in relation to the zygomaticomaxillary buttress (ZMB), in order to suggest another anatomical consideration and surgical modification of sinus floor elevation procedures. This study was designed as a cross-sectional study, and a total of 134 patients and 161 sinuses containing edentulous alveolar ridges were analyzed. The angle between the anterior and lateral walls of the Mx sinus (lateral sinus angle [LSA]), and the angle between the midpalatal line and the anterior sinus wall (anterior sinus angle [ASA]) were measured. Mean LSAs and ASAs were 105.9° ± 9.86° and 58.4° ± 6.43°, respectively. No significant difference between left and right sides was found (LSA, P = .420; right = 105.5° ± 9.27°; left = 105.5° ± 9.27° and ASA, P = .564; right = 57.9° ± 6.80°; left = 58.8° ± 6.02°). The prevalence of septa was 37.3%, and it was most frequently noted in the second molar region (32.8%), followed by the first molar (20.9%), retromolar (16.4%), and second premolar regions (14.9%). Septa were most frequently located posterior to the ZMB (49.2%), while ZMB was mostly located in the first molar region (66.4%). Narrow LSAs may complicate the surgical approach to the posterior maxilla, especially when sinus elevation should be used in the second molar region. Considering the occasional presence of antral septa, membrane elevation may be complicated when a septum is encountered during the procedure. These results suggest that 3-dimensional examination of the convexity of the Mx sinus should be performed preoperatively to choose proper surgical techniques and minimize surgical complications.

Setting the Sagittal Condylar Inclination on a Virtual Articulator Using Intraoral Scan of Protrusive Interocclusal Position and Cone Beam Computed Tomography.

The articulator is a prerequisite device for the analysis of occlusion and prosthodontic treatment, and it is required to simulate patient jaw movements. This article describes the technique to obtain sagittal condylar inclination (SCI) using cone beam computed tomography (CBCT) data and intraoral scan of the protrusive interocclusal position. The SCI can be used on a virtual articulator in a computer-aided design software, and it can assist in the fabrication of prosthesis which is harmonious with the mandibular movement of individual patient.

Association between MicroRNA-4669 Polymorphism and Ischemic Stroke in a Korean Population.

Recent studies have explored the association between single-nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) and ischemic stroke (IS). In particular, the associations of rs2910164 (miRNA-146A), rs11614913 (miRNA-196A2), and rs3746444 (miRNA-499A) were intensively studied in IS. In this study, we investigated the associations between SNPs in miRNAs and IS including rs2910164, rs11614913, and rs3746444 in a Korean population. For a pilot study, we selected 19 SNPs in pre-miRNA region (including mature miRNA region) and genotyped in 140 IS patients and 240 control subjects using the Fluidigm Dynamic Array. Our pilot study showed a weak association of rs79402775 in miRNA-933 (p = 0.044) and a relatively strong association of rs35196866 in miRNA-4669 (p = 0.016) with IS. From the pilot study, we selected rs79402775, rs35196866, and rs7202008 (miRNA-2117; p = 0.055) as candidate miRNA SNPs on IS and further genotyped these SNPs in 264 IS patients and 455 control subjects using direct sequencing. In addition, we further analyzed the associations of rs2910164, rs11614913, and rs3746444 that have been intensively studied in previous studies. In the further analysis, we found the significant association between rs35196866 and IS (p = 0.0014 in additive model and p = 0.00015 in dominant model; p = 0.00037 in allele frequency analysis). However, the association between rs2910164, rs11614913, rs3746444, rs79402775, and rs7202008 and IS was not shown. These results suggest that miRNA-4669 may be involved in the susceptibility of IS.

Protective Effect of Citric Acid Against Hepatic Ischemia Reperfusion Injury in Sprague-Dawley Rats.

OBJECTIVE: Hepatic ischemia reperfusion (I/R) injury is regarded as a serious concern in clinical practice. Citric acid reduces oxidative stress and inflammation during hypoxia and reoxygenation. Our objective was to investigate the protective effect of citric acid against hepatic I/R injury in rats. METHODS: We fed Sprague-Dawley rats either citric acid (100 mg/kg/d) or saline. One week later, ischemia was induced by clamping the rats' common hepatic artery and portal vein for 30 minutes. The rats were randomly divided into 3 major groups that were treated as follows: 1. the sham operated group; 2. the I/R group; and 3. the I/R-citric acid group. RESULTS: Compared to the sham group, the I/R group had higher expression of aspartate aminotransferase and alanine aminotransferase and lower expression of catalase, superoxide dismutase, glutathione peroxidase, antioxidant, nitric oxide, and albumin. Compared to the I/R group, the I/R-citric acid group had higher expression of catalase, superoxide dismutase, antioxidants, and nitric oxide, and lower expression of aspartate aminotransferase and alanine aminotransferase. CONCLUSIONS: These results suggest that citric acid therapy has significant therapeutic potential in ischemic liver injury.

Promoter Polymorphism (-308G/A) of Tumor Necrosis Factor-Alpha (TNF-α) Gene and Asthma Risk: An Updated Meta-Analysis.

Background and Aims: The relationship between the promoter polymorphism (-308G/A) of the tumor necrosis factor-alpha (TNF-α) gene and the susceptibility to asthma has been tested in several studies. However, the results have been inconsistent. Therefore, we performed an updated meta-analysis to evaluate the relationship between this promoter polymorphism of the TNF-α gene and the risk of asthma. Methods: Fifty case-control studies were included in this meta-analysis which provided 17,937 controls and 9961 asthma patients. The pooled p-value, odds ratio (OR), and 95% confidence interval (95% CI) were used to investigate the strength of the association of this polymorphism of the TNF-α gene with the risk of asthma. The meta-analysis was carried out by Comprehensive Meta-Analysis software. Results: The results of our meta-analysis revealed that the TNF-α polymorphism (-308, G/A) was strongly associated with the risk of asthma (p < 0.05 in the allelic, dominant, and recessive models, respectively). In further analyses, based on age group and ethnicity, we observed this association for all subpopulations examined (p < 0.05 in allelic, dominant, and recessive models, respectively). Conclusion: This large-scale meta-analysis supports a strong association between the TNF-α gene promoter polymorphism (-308G/A) and the development to asthma in both children and adults.