The effect of fasting on liveweight and carcass characteristics in lambs.

In two experiments, involving 250 and 300 lambs, respectively, lambs were fasted (with access to water) and slaughtered after 0, 1, 2, 3 and 4 days. The effects of initial fat score, initial liveweight and sex (wethers and ewes) on the patterns of loss in liveweight, hot carcass weight and carcass characters (fat depth at the GR and C sites, kidney fat weight and the weights of chemical carcass components) were examined. In experiment 2 the effects of weaning and transport on fasting losses were also examined. The rate of liveweight loss (expressed as a percentage of initial liveweight) was greater in the initial stages of the fast and was affected by fat score, with a greater loss in lambs with a lower fat score. The pattern of loss in hot carcass weight with fasting was curvilinear and in experiment 2 was affected by both fat score and liveweight, with leaner, heavier lambs having a greater loss. In experiment 2 the decrease in fat depth at the GR site interacted with fat score, with a greater loss in lambs with a lower fat score. Transport per se had no effect on hot carcass weight or carcass characteristics.

[Tissue antigens in ovarian carcinoma]. / Gewebsantigene beim Ovarialkarzinom.

Five tissue antigens have been identified which appear in carcinoma arising in the ovary. Antisera to a variety of human tissues were absorbed with a saline extract of normal ovary as well as plasma; five distinct antigens (A1-A4, TA) were detected in ovarian tumor extracts as well as in normal tissues. A1, A2, and A3 were widely distributed in normal tissues while A4 was found principally in buffy coat blood cells and spleen, and TA was mainly associated with normal cervix but was variably detected in liver and lung. Heating the tumor extract to 56 degrees resulted in inactivation of A1, A2, and A3. A4 was inactivated at 80 degrees, whereas TA was stable at 100 degrees. Pronase treatment readily abolished A3 activity, more slowly inactivated A1, A2, and A4, and only very slowly destroyed TA. Partial separation of the antigens was obtained by Sephadex G-200 chromatography. TA from individual benign and malignant tumors as well as normal cervix appears to be the same molecule by criteria of immunodiffusion, immunoelectrophoresis, and gel filtration. The potential use of these antigens as tracers of ovarian carcinoma is indicated.

Tissue antigens in ovarian carcinoma.

Five tissue antigens have been identified which appear in carcinoma arising in the ovary. Antisera to a variety of human tissues were absorbed with a saline extract of normal ovary as well as plasma; five distinct antigens (A1-A4, TA) were detected in ovarian tumor extracts as well as in normal tissues. A1, A2, and A3 were widely distributed in normal tissues while A4 was found principally in buffy coat blood cells and spleen, and Ta was mainly associated with normal cervix but was variably detected in liver and lung. Heating the tumor extract to 56 degrees resulted in inactivation of A1, A2, and A3. A4 was inactivated at 80 degrees, whereas TA was stable at 100 degrees. Pronase treatment readily abolished A3 activity, more slowly inactivated A1, A2, and A4, and only very slowly destroyed TA. Partial separation of the antigens was obtained by Sephadex G-200 chromatography. TA from individual benign and malignant tumors as well as normal cervix appears to be the same molecule by criteria of immunodiffusion, immunoelectrophoresis, and gel filtration. The potential use of these antigens as tracers of ovarian carcinoma is indicated.

A thermostable antigen associated with ovarian cancer.

Ovarian carcinoma contains an antigen (TA) which is stable at 100 degrees. Rabbit antisera to glycoprotein-rich extracts of tumors detect TA in 70 per cent of ovarian malignancies, in some benign ovarian cysts, certain normal lung preparations, normal cervix, and squamous-cell carcinoma of the cervix. Highest levels may be associated with mucin secretion. No detectible antigen was present in normal ovary, plasma, A, B, and O erythrocytes, leukocytes, placenta, brain, heart, liver, corpus uteri, spleen, skeletal muscle, or kidney. Prolonged digestion of boiled tumor extracts with papain, trypsin, chymotrypsin, on Sephadex G-150 corresponding to a globular protein of 27,000 to 36,000 molecular weight. A beta-globulin mobility is seen in immunoelectrophoresis. It appears that TA differs in tissue specificity and molecular size from other known ovarian cancer associated antigens.