Repeat Effort Performance Is Reduced 24 Hours After Acute Dehydration in Mixed Martial Arts Athletes.

Barley, OR, Iredale, F, Chapman, DW, Hopper, A, and Abbiss, C. Repeat effort performance is reduced 24 hours after acute dehydration in mixed martial arts athletes. J Strength Cond Res 32(9): 2555-2561, 2018-This study sought to determine the influence of acute dehydration on physical performance and physiology in mixed martial arts (MMA). Mixed martial arts athletes (n = 14; age: 23 ± 4 years) completed in a randomized counterbalanced order a dehydration protocol, (DHY: 3-hour cycling at 60 W in 40° C to induce 5% dehydration) or thermoneutral control (25° C: CONT) exercise, followed by ad libitum fluid/food intake. Performance testing (a repeat sled push test, medicine ball chest throw, and vertical jump) was completed 3 hours and 24 hours after the intervention, whereas urine and blood samples were collected before, 20 minutes, 3 hours, and 24 hours after the intervention. Body mass was reduced (4.8 ± 0.8%) after DHY (p < 0.001) and remained lower than CONT at 3 hours and 24 hours after DHY (p = 0.003 and p = 0.024, respectively). Compared with CONT, average sled push times were slower 3 hours and 24 hours after DHY (19 ± 15%; p = 0.001; g = 1.229 and 14 ± 15%; p = 0.012; g = 0.671, respectively). When compared with the CONT, handgrip was weaker 3 hours after DHY (53 ± 8 and 51 ± 8 kg; p = 0.044, g = 0.243, respectively) and medicine ball chest throw distances were shorter 24 hours after DHY (474 ± 52 and 449 ± 44 cm; p = 0.016, g = 0.253, respectively). No significant differences were observed in vertical jump (p = 0.467). Urine specific gravity was higher than CONT 20 minutes (p = 0.035) and 24 hours (p = 0.035) after DHY. Acute dehydration of 4.8% body mass results in reduced physical performance 3 and 24 hours after DHY. There is need for caution when athletes use dehydration for weight loss 24 hours before competition.

Neuromuscular Training Improves Lower Extremity Biomechanics Associated with Knee Injury during Landing in 11-13 Year Old Female Netball Athletes: A Randomized Control Study.

The purpose of this study was to examine the effects of a neuromuscular training (NMT) program on lower-extremity biomechanics in youth female netball athletes. The hypothesis was that significant improvements would be found in landing biomechanics of the lower-extremities, commonly associated with anterior cruciate ligament (ACL) injury, following NMT. Twenty-three athletes (age = 12.2 ± 0.9 years; height = 1.63 ± 0.08 m; mass = 51.8 ± 8.5 kg) completed two testing sessions separated by 7-weeks and were randomly assigned to either a experimental or control group. Thirteen athletes underwent 6-weeks of NMT, while the remaining 10 served as controls and continued their regular netball training. Three-dimensional lower-extremity kinematics and vertical ground reaction force (VGRF) were measured during two landing tasks, a drop vertical jump and a double leg broad jump with a single leg landing. The experimental group significantly increased bilateral knee marker distance during the bilateral landing task at maximum knee-flexion range of motion. Knee internal rotation angle during the unilateral landing task at maximum knee flexion-extension range of motion was significantly reduced (p ≤ 0.05, g > 1.00). The experimental group showed large, significant decreases in peak vertical ground reaction force in both landing tasks (p ≤ 0.05, g > -1.30). Control participants did not demonstrate any significant pre-to-post-test changes in response to the 6-week study period. Results of the study affirm the hypothesis that a 6-week NMT program can enhance landing biomechanics associated with ACL injury in 11-13 year old female netball athletes.

The efficacy of periodised resistance training on neuromuscular adaptation in older adults.

PURPOSE: This study compared the effect of periodised versus non-periodised (NP) resistance training on neuromuscular adaptions in older adults. METHODS: Forty-one apparently healthy untrained older adults (female = 21, male = 20; 70.9 ± 5.1 years; 166.3 ± 8.2 cm; 72.9 ± 13.4 kg) were recruited and randomly stratified to an NP, block periodised (BP), or daily undulating periodised (DUP) training group. Outcome measures were assessed at baseline and following a 22-week resistance training intervention (3 day week-1), including: muscle cross-sectional area (CSA), vertical jump performance, isometric and isokinetic peak torque, isometric rate of force development (RFD), and muscle activation. Thirty-three participants satisfied all study requirements and were included in analyses (female = 17, male = 16; 71.3 ± 5.4 years; 166.3 ± 8.5 cm; 72.5 ± 13.7 kg). RESULTS: Block periodisation, DUP, and NP resistance training induced statistically significant improvements in muscle CSA, vertical jump peak velocity, peak power and jump height, and peak isometric and isokinetic torque of the knee extensors at 60 and 180° s-1, with no between-group differences. Muscle activity and absolute RFD measures were statistically unchanged following resistance training across the entire cohort. CONCLUSIONS: Periodised resistance training, specifically BP and DUP, and NP resistance training are equally effective for promoting increases in muscular hypertrophy, strength, and power among untrained older adults. Consequently, periodisation strategies are not essential for optimising neuromuscular adaptations during the initial stages of resistance training in the aging population.

Neuromuscular Training Improves Movement Competency and Physical Performance Measures in 11-13-Year-Old Female Netball Athletes.

Hopper, A, Haff, EE, Barley, OR, Joyce, C, Lloyd, RS, and Haff, GG. Neuromuscular training improves movement competency and physical performance measures in 11-13-year-old female netball athletes. J Strength Cond Res 31(5): 1165-1176, 2017-The purpose of this study was to examine the effects of a neuromuscular training (NMT) program on movement competency and measures of physical performance in youth female netball players. It was hypothesized that significant improvements would be found in movement competency and physical performance measures after the intervention. Twenty-three junior female netball players (age, 12.17 ± 0.94 years; height, 1.63 ± 0.08 m; weight, 51.81 ± 8.45 kg) completed a test battery before and after a 6-week training intervention. Thirteen of these athletes underwent 6 weeks of NMT, which incorporated plyometrics and resistance training. Trained athletes showed significant improvements in 20-m sprint time, 505 agility time, countermovement jump height, and peak power (p ≤ 0.05, g > 0.8). In addition, trained athletes significantly improved their score in the Netball Movement Screening Tool (NMST) (p < 0.05, g > -1.30); while the athletes also demonstrated increased reach in the anterior and posteromedial directions for the right leg and left leg, and in the posterolateral direction for the left leg only in the Star Excursion Balance Test (SEBT) (p < 0.05, g > -0.03). Control subjects did not exhibit any significant changes during the 6-week period. Significant negative correlations were found between improved score on the NMST and decreased 5-, 10-, and 20-m sprint time, and 505 change of direction time (r > 0.4, p ≤ 0.05). Results of the study affirm the hypothesis that a 6-week NMT intervention can improve performance and movement competency in youth netball players.

Cluster Sets: Permitting Greater Mechanical Stress Without Decreasing Relative Velocity.

PURPOSE: To determine the effects of intraset rest frequency and training load on muscle time under tension, external work, and external mechanical power output during back-squat protocols with similar changes in velocity. METHODS: Twelve strength-trained men (26.0 ± 4.2 y, 83.1 ± 8.8 kg, 1.75 ± 0.06 m, 1.88:0.19 one-repetition-maximum [1RM] body mass) performed 3 sets of 12 back squats using 3 different set structures: traditional sets with 60% 1RM (TS), cluster sets of 4 with 75% 1RM (CS4), and cluster sets of 2 with 80% 1RM (CS2). Repeated-measures ANOVAs were used to determine differences in peak force (PF), mean force (MF), peak velocity (PV), mean velocity (MV), peak power (PP), mean power (MP), total work (TW), total time under tension (TUT), percentage mean velocity loss (%MVL), and percentage peak velocity loss (%PVL) between protocols. RESULTS: Compared with TS and CS4, CS2 resulted in greater MF, TW, and TUT in addition to less MV, PV, and MP. Similarly, CS4 resulted in greater MF, TW, and TUT in addition to less MV, PV, and MP than TS did. There were no differences between protocols for %MVL, %PVL, PF, or PP. CONCLUSIONS: These data show that the intraset rest provided in CS4 and CS2 allowed for greater external loads than with TS, increasing TW and TUT while resulting in similar PP and %VL. Therefore, cluster-set structures may function as an alternative method to traditional strength- or hypertrophy-oriented training by increasing training load without increasing %VL or decreasing PP.

Periodization Strategies in Older Adults: Impact on Physical Function and Health.

PURPOSE: This study compared the effect of periodized versus nonperiodized (NP) resistance training (RT) on physical function and health outcomes in older adults. METHODS: Forty-one apparently healthy untrained older adults (women = 21, men = 20; 70.9 ± 5.1 yr; 166.3 ± 8.2 cm; 72.9 ± 13.4 kg) were recruited and randomly stratified to a NP, block periodized, or daily undulating periodized training group. Outcome measures were assessed at baseline and after a 22-wk × 3 d·wk RT intervention, including; anthropometrics, body composition, blood pressure and biomarkers, maximal strength, functional capacity, balance confidence, and quality of life. RESULTS: Thirty-three subjects satisfied all study requirements and were included in analyses (women = 17, men = 16; 71.3 ± 5.4 yr; 166.3 ± 8.5 cm; 72.5 ± 13.7 kg). The main finding was that all three RT models produced significant improvements in several physical function and physiological health outcomes, including; systolic blood pressure, blood biomarkers, body composition, maximal strength, functional capacity and balance confidence, with no between-group differences. CONCLUSIONS: Periodized RT, specifically block periodization and daily undulating periodized, and NP RT are equally effective for promoting significant improvements in physical function and health outcomes among apparently healthy untrained older adults. Therefore, periodization strategies do not appear to be necessary during the initial stages of RT in this population. Practitioners should work toward increasing RT participation in the age via feasible and efficacious interventions targeting long-term adherence in minimally supervised settings.

A critical role for the cccA gene product, cytochrome c2, in diverting electrons from aerobic respiration to denitrification in Neisseria gonorrhoeae.

Neisseria gonorrhoeae is a microaerophile that, when oxygen availability is limited, supplements aerobic respiration with a truncated denitrification pathway, nitrite reduction to nitrous oxide. We demonstrate that the cccA gene of Neisseria gonorrhoeae strain F62 (accession number NG0292) is expressed, but the product, cytochrome c2, accumulates to only low levels. Nevertheless, a cccA mutant reduced nitrite at about half the rate of the parent strain. We previously reported that cytochromes c4 and c5 transfer electrons to cytochrome oxidase cbb3 by two independent pathways and that the CcoP subunit of cytochrome oxidase cbb3 transfers electrons to nitrite. We show that mutants defective in either cytochrome c4 or c5 also reduce nitrite more slowly than the parent. By combining mutations in cccA (Δc2), cycA (Δc4), cycB (Δc5), and ccoP (ccoP-C368A), we demonstrate that cytochrome c2 is required for electron transfer from cytochrome c4 via the third heme group of CcoP to the nitrite reductase, AniA, and that cytochrome c5 transfers electrons to nitrite reductase by an independent pathway. We propose that cytochrome c2 forms a complex with cytochrome oxidase. If so, the redox state of cytochrome c2 might regulate electron transfer to nitrite or oxygen. However, our data are more consistent with a mechanism in which cytochrome c2 and the CcoQ subunit of cytochrome oxidase form alternative complexes that preferentially catalyze nitrite and oxygen reduction, respectively. Comparison with the much simpler electron transfer pathway for nitrite reduction in the meningococcus provides fascinating insights into niche adaptation within the pathogenic neisseriae.

Organization of the electron transfer chain to oxygen in the obligate human pathogen Neisseria gonorrhoeae: roles for cytochromes c4 and c5, but not cytochrome c2, in oxygen reduction.

Although Neisseria gonorrhoeae is a prolific source of eight c-type cytochromes, little is known about how its electron transfer pathways to oxygen are organized. In this study, the roles in the respiratory chain to oxygen of cytochromes c(2), c(4), and c(5), encoded by the genes cccA, cycA, and cycB, respectively, have been investigated. Single mutations in genes for either cytochrome c(4) or c(5) resulted in an increased sensitivity to growth inhibition by excess oxygen and small decreases in the respiratory capacity of the parent, which were complemented by the chromosomal integration of an ectopic, isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible copy of the cycA or cycB gene. In contrast, a cccA mutant reduced oxygen slightly more rapidly than the parent, suggesting that cccA is expressed but cytochrome c(2) is not involved in electron transfer to cytochrome oxidase. The deletion of cccA increased the sensitivity of the cycB mutant to excess oxygen but decreased the sensitivity of the cycA mutant. Despite many attempts, a double mutant defective in both cytochromes c(4) and c(5) could not be isolated. However, a strain with the ectopically encoded, IPTG-inducible cycB gene with deletions in both cycA and cycB was constructed: the growth and survival of this strain were dependent upon the addition of IPTG, so gonococcal survival is dependent upon the synthesis of either cytochrome c(4) or c(5). These results define the gonococcal electron transfer chain to oxygen in which cytochromes c(4) and c(5), but not cytochrome c(2), provide alternative pathways for electron transfer from the cytochrome bc(1) complex to the terminal oxidase cytochrome cbb(3).

A physiologically significant role in nitrite reduction of the CcoP subunit of the cytochrome oxidase cbb3 from Neisseria gonorrhoeae.

The CcoP subunit of cytochrome oxidase cbb(3) of Neisseria gonorrhoeae is predicted to include a C-terminal extension in which there is a C-A-A-C-H- motif typical of heme attachment sites in c-type cytochromes. Substitutions of key cysteine and histidine residues of this motif resulted in mutants that grew normally in oxygen-sufficient cultures and reduced oxygen at the same rate as the parent strain. In contrast, after oxygen-limited growth in the presence of nitrite, rates of nitrite reduction were significantly lower than those of the parent, consistent with a role for this third heme-binding domain in electron transfer to the nitrite reductase, AniA, located in the outer membrane. As the mutants were still able to reduce nitrite at approximately 65% of the rate of the parent, there are multiple pathways in the gonococcus for electron transfer to AniA. On the basis of sequence similarity between the C-terminal extension of CcoP and cytochrome c(5), it is proposed that cytochrome c(5) might also transfer electrons across the periplasm from the cytochrome bc(1) complex in the cytoplasmic membrane to AniA in the outer membrane. This is the first example of a cytochrome oxidase component that plays a physiologically significant role in nitrite reduction.