Carbon isotope fractionation in karst aquatic mosses.

Radiocarbon activity (a14C) and 13C composition (δ13C) were measured in hygrophyte and mesophyte (land) mosses collected in the natural habitat of the Plitvice Lakes and along the Zrmanja and Krupa Rivers (typical continental and Mediterranean climates, respectively), Croatia. a14C and δ13C values of mosses, of atmospheric CO2 and dissolved inorganic carbon (DIC) were compared with contemporary data and with data from 30 years ago at the Plitvice Lakes when 14C activity of atmospheric CO2 was ∼30% higher. A positive correlation between a14Cmoss and δ13Cmoss was observed for all data reflecting the change of carbon isotopic composition in DIC along the water flows and in atmospheric CO2 regardless of the climatic regions and historic period. Fraction of the atmospheric carbon in moss (ωatm.C) and carbon fractionation factor from aquatic CO2 (DIC) to moss tissue (εmoss/g-aq) were calculated for each individual moss. Three species of mosses had ωatm.C ∼ 0 % implying that they turn to anabiosis during dry periods. The relation εmoss/g-aqvs.ωatm.C differentiates true aquatic and amphiphyte mosses. The first had a statistically significant negative correlation between εmoss/g-aq and ωatm.C. The amphiphyte mosses had lower εmoss/g-aq with higher water flow rates.

Spatial and seasonal variations in the stable C isotope composition of dissolved inorganic carbon and in physico-chemical water parameters in the Plitvice Lakes system.

Plitvice Lakes waters were collected at 14 sampling points, including springs, tributaries and lakes, for the period 2002-2007. The results of the physical and chemical conditions of calcite precipitation as well as the δ(13)C values of dissolved inorganic carbon (DIC) were used to study the processes influencing calcite precipitation. Significant differences between spring, lake and stream waters as well as changes in the downstream direction were observed. The correlation between δ(13)C(DIC) values and physico-chemical conditions for calcite precipitation showed that calcite precipitates in lake waters which are oversaturated with respect to CaCO(3) (I (sat) values 4-10) and with δ(13)C(DIC) values between-11.5 and-8.5 ‰. In spring waters, the δ(13)C(DIC) values were more negative, from-14 to-12 ‰, and I (sat) values of 1-2 indicated that equilibrium conditions for calcite precipitation were not attained. The downstream increase in δ(13)C(DIC) correlated with the increase in the δ(13)C values of calcite in the lake sediments, suggesting that the freshwater calcite was mainly of autochthonous origin and precipitated within the water column in isotopic equilibrium with DIC.

Stable isotope composition of daily and monthly precipitation in Zagreb.

Stable isotopic compositions (delta2H and delta18O) of daily precipitation collected in the period from October 2002 to March 2003 and monthly precipitation in the period from 2001 to 2003, as well as the corresponding meteorological data (temperature, amount of precipitation), all collected in Zagreb, Croatia, are presented. delta2H and delta18O values, both daily and monthly, show large variations due to large temperature variations and the different origin of the air masses. Variations are larger for daily samples than for composite monthly samples. Good correlation of delta18O with temperature is obtained for both types of samples. On the basis of the correlation between delta2H and delta18O, the local meteoric water line is close to the global meteoric water line. Deuterium excess of both daily and monthly precipitation indicates that in the Zagreb area, the influence of air masses from the Mediterranean area prevails in the autumn period.

Distribution of hydrogen, oxygen and carbon isotopes in the atmosphere of Croatia and Slovenia.

Monitoring of isotope distribution in the atmosphere included isotopes 2H, 3H and 18O in precipitation, and 13C and 14C in atmospheric CO2. This paper presents the results of atmospheric monitoring over Croatia and Slovenia overt the last few years. Monthly precipitations in Zagreb and Ljubljana had been collected since 1976 and 1981, respectively. Between 2000 and 2003 monitoring also included seven stations along the Adriatic coast. Tritium activity in precipitation shows seasonal variations that were most pronounced inland (at the continental stations Zagreb and Ljubljana) and were the smallest in the south Adriatic (Komiza and Dubrovnik). Mean annual tritium activity decreases from the north to the south Adriatic coast. Seasonal variations in delta2H and delta18O in precipitation follow the temperature variations at the sampling stations. Atmospheric CO2 was collected in Zagreb and Plitvice as a composite monthly sample. Both delta14C and delta13C showed seasonal variations. The mean annual 14C activities in Zagreb had been decreasing after the peak reached in the 1960s, and for the last three years the mean 14C activity (delta14C) remained about 30 per thousand.