RESUMO
The B 10 strain of mice was used to test the effect of the boar seminal vesicle immunosuppressive factor on the female mouse response to the male-specific transplantation antigen. Influence of this factor on human natural killer (NK) cell activity was also studied. No inhibitory effect of the immunosuppressive factor on graft survival was apparent during a time of more than 200 days, nor did the factor suppress NK cell activity.
Assuntos
Sobrevivência de Enxerto/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Proteínas Secretadas pela Próstata , Proteínas/farmacologia , Sêmen/química , Transplante de Pele , Animais , Feminino , Humanos , Masculino , Camundongos , Proteínas de Plasma Seminal , Caracteres Sexuais , Suínos , Imunologia de Transplantes/efeitos dos fármacosRESUMO
Boar seminal vesicle fluid inhibits blast transformation of porcine lymphocytes. Boar seminal vesicle fluid was precipitated in 8% ethanol and the dissolved precipitate separated into two peaks upon chromatography on a Sephacryl S-200 column. The inhibitory activity was found predominantly in the second peak. This peak was rechromatographed on the Sephacryl S-200 column, and the fractions with inhibitory activity were pooled and passed down a Sephadex G-75 column. This chromatography run separated the accompanying protease inhibitor from the immunosuppressive fraction. The molecular mass, estimated by chromatography on the Sephadex G-75 column, was 25,000-27,000 for the immunosuppressive factor and 7,500 for the protease inhibitor. Activity for the immunosuppressive fraction was also demonstrated in vivo.
