RESUMO
OBJECTIVE: To observe the mechanism of SHU-Dihuang on the function of learning and memory. METHOD: On the dementia model mouse caused by AlCl3 and the rats model damaged thalamic arcuate nucleus with MSG, we observed the function of learning and memory by step down task and Morris water maze task, mensurated the content of glutamic acid and gamma-aminobutyric acid by TLC, and observed the expression of NMDAR1 and GABAR in hippocampi by immunohistochemical means. RESULT: Shu Di Huang could decrease the times of mistakes and prolong the incubation period in step down task, and shorten the incubation period of seeking the platform in Morris water maze task. Shu Di Huang could adjust the content of Glu and GABA in brain, and increase the expression of hippocampal NMDAR1 and GABAR as well. CONCLUSION: Shu Di Huang can improve the function of learning and memory of dementia animal model, and its mechanism may be related to the adjustment of the content of Glu and GABA in brain, and increase of the expression of hippocampal NMDAR1 and GABAR.
Assuntos
Demência/fisiopatologia , Medicamentos de Ervas Chinesas/farmacologia , Hipocampo/metabolismo , Receptores de GABA/metabolismo , Rehmannia , Animais , Núcleo Arqueado do Hipotálamo/efeitos dos fármacos , Demência/induzido quimicamente , Medicamentos de Ervas Chinesas/isolamento & purificação , Feminino , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Camundongos , Plantas Medicinais/química , Ratos , Ratos Sprague-Dawley , Receptores de N-Metil-D-Aspartato/metabolismo , Rehmannia/químicaRESUMO
OBJECTIVE: To study the mechanism of Shu Di-huang in improving the function of learning and memory. METHOD: On the rats model with thalamic arcuate nucleus dameged by MSG, the improving function of Shu Di-huang on learning and memory was observed by step down task and Morris water maze task, and the expression of c-fos and NGF in hippocampi was observed by immunohistochemical means. RESULT: Shu Di-huang could decrease the times of mistakes and prolong the incubation period in step down task, and shorten the incubation period of seeking the platform, and improve the percentage rate through the platform position in Morris water maze task. Shu Di-huang also increase the expression of hippocampal NGF, c-fos. CONCLUSION: Shu Di-huang can improve the function of learning and memory of MSG rats, and its mechanism may be related with the increase of the expression of hippocampal c-fos and NGF.
