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1.
Methods Mol Biol ; 1572: 365-377, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28299700

RESUMO

This study presents a novel microfluidic approach to the investigation of bacterial susceptibility against antibiotics gradient concentration in a quantitative way. A linear concentration gradient of antibiotics in a microfluidic chamber is used with time-lapse photography. This approach provides high-resolution information in real time. Traditional tests for this type of study involve Clinical and Laboratory Standard Institute (CLSI) recommended test and Etest. All of these tests need different trials to present different concentrations. They can only provide either static data or few sampling points in a time-consuming way. Our approach employes a gradient generated structure to perform thousands of trials in parallel, showing the approach can give results in rapidly, efficiently and reveal the dynamics.


Assuntos
Testes de Sensibilidade Microbiana/instrumentação , Testes de Sensibilidade Microbiana/métodos , Técnicas Analíticas Microfluídicas/instrumentação , Microfluídica/instrumentação , Desenho de Equipamento , Microscopia , Reprodutibilidade dos Testes , Software , Estatística como Assunto/métodos , Imagem com Lapso de Tempo/métodos
2.
Micromachines (Basel) ; 7(11)2016 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-30404375

RESUMO

In microfluidic device prototyping, master fabrication by traditional photolithography is expensive and time-consuming, especially when the design requires being repeatedly modified to achieve a satisfactory performance. By introducing a high-performance/cost-ratio laser to the traditional soft lithography, this paper describes a flexible and rapid prototyping technique for microfluidics. An ultraviolet (UV) laser directly writes on the photoresist without a photomask, which is suitable for master fabrication. By eliminating the constraints of fixed patterns in the traditional photomask when the masters are made, this prototyping technique gives designers/researchers the convenience to revise or modify their designs iteratively. A device fabricated by this method is tested for particle separation and demonstrates good properties. This technique provides a flexible and rapid solution to fabricating microfluidic devices for non-professionals at relatively low cost.

3.
Lab Chip ; 14(17): 3409-18, 2014 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-25007721

RESUMO

This study reports a novel approach to quantitatively investigate the antibacterial effect of antibiotics on bacteria using a three-dimensional microfluidic culture device. In particular, our approach is suitable for studying the pharmacodynamics effects of antibiotics on bacterial cells temporally and with a continuous range of concentrations in a single experiment. The responses of bacterial cells to a linear concentration gradient of antibiotics were observed using time-lapse photography, by encapsulating bacterial cells in an agarose-based gel located in a commercially available microfluidics chamber. This approach generates dynamic information with high resolution, in a single operation, e.g., growth curves and antibiotic pharmacodynamics, in a well-controlled environment. No pre-labelling of the cells is needed and therefore any bacterial sample can be tested in this setup. It also provides static information comparable to that of standard techniques for measuring minimum inhibitory concentration (MIC). Five antibiotics with different mechanisms were analysed against wild-type Escherichia coli, Staphylococcus aureus and Salmonella Typhimurium. The entire process, including data analysis, took 2.5-4 h and from the same analysis, high-resolution growth curves were obtained. As a proof of principle, a pharmacodynamic model of streptomycin against Salmonella Typhimurium was built based on the maximal effect model, which agreed well with the experimental results. Our approach has the potential to be a simple and flexible solution to study responding behaviours of microbial cells under different selection pressures both temporally and in a range of concentrations.


Assuntos
Antibacterianos/farmacologia , Testes de Sensibilidade Microbiana , Técnicas Analíticas Microfluídicas/instrumentação , Escherichia coli/efeitos dos fármacos , Salmonella typhimurium/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
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