Analysis of flavonoids and antitumor activity of transgenic Saussurea involucrate / 中国中药杂志

The aim of this paper was to investigate the flavonoids of callus of transgenic and non-transgenic Saussurea involucrate and its antitumor activity on the esophageal cancer CaEs-17 cells. The species and content of mono-phenols were detected by high performance liquid chromatography. The growth of human esophageal cancer CaEs-17 cells was detected using CCK-8 assay, apoptosis morphology observation and flow cytometry. Expression of related apoptotic genes Bax and Bcl-2 were determined by qPCR. The results showed that the content of total flavonoids in the transgenic callus was 2.72 times that of the non-transgenic callus. The cyanidin-galactoside was detected in the transgenic callus, but not in the non-transgenic callus. The inhibitory effect of the extracts from the transgenic callus on CaEs-17 cells was more significant than that of the non-transgenic callus, and the IC₅₀ value had a decreased of 26.4%. Flow cytometry analysis results showed that the apoptosis induction effect of the extracts from the transgenic callus on CaEs-17 cells was significantly better than that of the non-transgenic callus. Fluorescence quantitative PCR analysis results showed that the extracts from the transgenic callus could up-regulate the expression of proapoptotic gene Bax and down-regulate the expression of apoptotic gene Bcl-2, and the regulation effect of the transgenic callus was more significant. Therefore, compared with the non-transgenic callus, the antitumor activity of the extracts from the callus of transgenic S. involucrate on the esophageal cancer CaEs-17 cells was significantly increased, which was closely related to the accumulation of cyanidin-galactoside and its metabolism-related flavonoid compounds in the transgenic callus.

[Interaction of cucurbit [n = 7, 8] with methylindoles via fluorescence spectroscopy].

In the present work, the authors investigated interaction of cucurbit [n=7, 8] urils(Q[7], Q[8]) with 2-methylindol (Me) and 3-methylindol(Sk), influences of pH and temperature on the interaction of Q[7], Q[8] with the guests and calculations of the stability constants and thermodynamic parameters by using fluorescence spctroscopy. The results show that: the interation ratios are 1 : 1 of host: guest for all systems, except the Q[8]-Sk system, for which the ratio is 1 : 2 of host: guest; the interaction ratios of the host: guest systems did not change in the range of pH 1-11 and temperature between 298 and 313 K; the stability constant is the largest at pH 1 and room temperature. The thermodynamic parameter revealed that the interaction of the host and the guest of spontaneitied.