Clinical efficacy and CT perfusion of puerarin combined with naloxone in the treatment of traumatic cerebral infarction.

Puerarin is an important active ingredient of Radix puerariae. Puerarin is an isoflavone compound Z clinical and basic research proves that S. puerarin can increase cerebral circulation blood flow; increase cerebral perfusion; reduce blood viscosity and improve microcirculation. The present research was conducted to observe the clinical efficacy of puerarin combined with naloxone in the treatment of traumatic cerebral infarction (TCI). Methods 52 patients with TCI were randomly divided into two groups. The treatment group was treated with puerarin and naloxone and the control group was treated with conventional therapy. Results: The total effective rate was 82% in the treatment group, which was better than 61% in the control group. The difference was statistically significant (p<0.05). The efficacy of simple TCI in both groups was significantly better than that of TCI with brain contusion; the effect of intracranial hemorrhage and the effect of TCI alone in the two groups; the treatment group was significantly better than the control group (p<0.05). Conclusion: The combination of puerarin and naloxone in the treatment of TCI can improve the curative effect.

IAP-1 gene silencing enhanced the radiosensitivity of liver cancer SMMC-7721 cells / 中华肝胆外科杂志

Objective To explore the effect of cellular inhibitor of apoptosis protein1 (cIAP-1) gene on the radiosensitivity of SMMC-7721 cells.Methods We silenced cIAP-1 expressions by the shRNA technology,and then we detected the changes of cell proliferation,cell cycle and cell apoptosis by CCK8 as-say,Western blot,qRT-PCR and flow cytometry after the radiotherapy.Results The cell proliferation rate of liver cancer SMMC-7721 cells at different radiation doses of 1 Gy,4 Gy,7 Gy and 10 Gy was detected.Comparing with control group (pGCsi-H1-control),the cell proliferation in cIAP-1 silencing group (pGCsiH1-shRNA) was significantly reduced at various radiation doses,and the effect was dose-dependent (P ＜ 0.05).G1/G0 phase arrest was observed after radiation (P ＜0.01),and the proportion of cells in S phase was significantly reduced compared with control (P ＜ 0.01).Compared with control group,G1/G0 phase arrest was detected (P ＜ 0.05),and the percentage of cell apoptosis was increased significantly in cIAP-1 silencing group (P ＜ 0.05).Conclusion cIAP-1 silencing can enhance the radiosensitivity of liver cancer cells,and inhibit cell proliferation by promoting the anti-tumor effect of radiation.

Study on the effect of transcather arterial chemoembolization combined with endostatin on the angiogenesis in rabbit VX2 tumor / 中华放射学杂志

Objective To investigate the effect on the miemvnscular density(MVD)and the expression of vascular endothelial growth factor(VEGF)in VX2 tumor after transcatheter arterial chemoembolization(TACE)combined with endostatin.Methods The VX2 tumor model wag established.The rabbits bearing tunlor were randomly divided into three groups as the control group.TACE group and TACE combined with endostatin group.with 10 rabbits in each group.In the control group,normal saline was administered via the hepatic artery.In the TACE group,lipiodol(0.2 ml/kg)and ADM(2.g/kg)were administered.Lipiodol(0.2 ml/kg),ADM(2 mg/kg)and endostatin(2 mg/kg)were administered for each rabbit in the TACE combined with endostatin group.Seven days after operation,the rabbits were sacrificed and the tumors were removed.Immunohistochemistry wag performed to demonstrate the expression of VEGF and the MVD wag caleulated.The ANOVA wag used for statistics.Results The average absorbance values of VEGF were 0.130±0.038.0.200±0.049 and 0.120±0.047 respectively for the 3 groups.There was signiflcant difference among the three groups(F=9.42,P＜0.01).rnle expression of VEGF in the TACE group was the hiShest among the 3 groups.Compared with the otIler two groups,there had signifieant differences(q=4.93,5.63,P＜0.01).The average absorbance value of the TACE combined with endostatin group was lower than that of control group,but there was no significant difference between them(q=0.70,P＞0.05).The values of MVD were(80±17),(84±16)and(57±13)/HPF for the 3 groups respectivelv.There wag significant difference among them(F=8.70,P＜0.01).111e value of MVD in the TACE combined with endestatin group was the lowest, and there were significant differences when compared with the control group (q=4.63, P＜0.01) and the TACE group (q =5.48, P＜0.01).There was no significant difference between the control group and the TACE group (q = 0.85, P ＞ 0.05) in MVD.Conclusion Compared with chemoembolization only, ehemoembolization combined with endostatin can significantly depress the expression of VEGF and decrease the angiogenesis of the tumor.The combined method has a beneficial effect on prognosis of hepatic tumor.

A Study on the Effect of TACE on the Apoptosis Proteins fas, bax and bcl-xl in HCC / 实用放射学杂志

Objective To explore the effect of transcatheter arterial chemoembolization ( TACE ) on the apoptosis proteins fas,bax,and bcl-xl in hepatocellular carcinoma ( HCC ) . Methods Sixty-three cases with HCC proved by histopathologically were studied, which included 42 cases treated with surgical resection alone,and 21 cases underwent TACE . The expressions of fas,bax,bcl-2 and bcl-xl were detected with immunohistochemical SP method .Results The positive rate of fas was 47.62% ( 10/21 ) in HCCs treated with TACE,and 21.43%(9/42) in those treated with surgical resection alone, there was significantly difference between the two groups(?0.05). Conclusion TACE can increase the expression of fas and decrease the expression of bcl-xl, it can increase the rate of apoptosis of HCC, make HCC lesion contract. The effect of TACE to bax need more research.