Age-related breakdown in networks of inter-muscular coordination.

Assessing inter-muscular coordination in older adults is crucial, as it directly impacts an individual's ability for independent functioning, injury prevention, and active engagement in daily activities. However, the precise mechanisms by which distinct muscle fiber types synchronize their activity across muscles to generate coordinated movements in older adults remain unknown. Our objective is to investigate how distinct muscle groups dynamically synchronize with each other in young and older adults during exercise. Thirty-five young adults and nine older adults performed one bodyweight squat set until exhaustion. Simultaneous surface electromyography (sEMG) recordings were taken from the left and right vastus lateralis, and left and right erector spinae. To quantify inter-muscular coordination, we first obtained ten time series of sEMG band power for each muscle, representing the dynamics of different muscle fiber types. Next, we calculated the bivariate equal-time Pearson's cross-correlation for each pair of sEMG band power time series across all leg and back muscles. The main results show (i) an overall reduction in the degree of inter-muscular coordination, and (ii) increased stratification of the inter-muscular network in older adults compared to young adults. These findings suggest that as individuals age, the global inter-muscular network becomes less flexible and adaptable, hindering its ability to reorganize effectively in response to fatigue or other stimuli. This network approach opens new avenues for developing novel network-based markers to characterize multilevel inter-muscular interactions, which can help target functional deficits and potentially reduce the risk of falls and neuro-muscular injuries in older adults.

Physical Exercise-Induced DNA Methylation in Disease-Related Genes in Healthy Adults-A Systematic Review With Bioinformatic Analysis.

ABSTRACT: Vasileva, F, Hristovski, R, Font-Lladó, R, Georgiev, G, Sacot, A, López-Ros, V, Calleja-González, J, Barretina-Ginesta, J, López-Bermejo, A, and Prats-Puig, A. Physical exercise-induced DNA methylation in disease-related genes in healthy adults-A systematic review with bioinformatic analysis. J Strength Cond Res 38(2): 384-393, 2024-This study aimed to systematically review the existing literature regarding physical exercise (PE) and DNA methylation (DNAm) in healthy adults. Specific goals were to (a) identify differently methylated genes (DMGs) after PE intervention, their imprinting status, chromosome and genomic location, function, and related diseases; and (b) to screen for core genes and identify methylation changes of the core genes that can be modified by PE intervention. Our search identified 2,869 articles from which 8 were finally included. We identified 1851 DMGs ( p < 0.05) after PE intervention, although 45 of them were imprinted. Aerobic exercise (AE) seems to induce more DNA hypermethylation rather than hypomethylation, whereas anaerobic exercise (AN) seems to induce more DNA hypomethylation rather than hypermethylation. Aerobic exercise induced highest % of methylation changes on chromosome 6, whereas AN and mixed type (MT) on chromosome 1. Mixed type induced higher % of methylation changes close to transcription start site in comparison to AE and AN. After PE intervention, DMGs were mainly involved in fat metabolism, cell growth, and neuronal differentiation, whereas diseases regulated by those genes were mainly chronic diseases (metabolic, cardiovascular, neurodegenerative). Finally, 19 core genes were identified among DMGs, all related to protein metabolism. In conclusion, our findings may shed some light on the mechanisms explaining PE-induced health benefits such as the potential role that PE-induced DNAm may have in disease prevention and disease treatment.

Network Physiology of Exercise: Beyond Molecular and Omics Perspectives.

Molecular Exercise Physiology and Omics approaches represent an important step toward synthesis and integration, the original essence of Physiology. Despite the significant progress they have introduced in Exercise Physiology (EP), some of their theoretical and methodological assumptions are still limiting the understanding of the complexity of sport-related phenomena. Based on general principles of biological evolution and supported by complex network science, this paper aims to contrast theoretical and methodological aspects of molecular and network-based approaches to EP. After explaining the main EP challenges and why sport-related phenomena cannot be understood if reduced to the molecular level, the paper proposes some methodological research advances related to the type of studied variables and measures, the data acquisition techniques, the type of data analysis and the assumed relations among physiological levels. Inspired by Network Physiology, Network Physiology of Exercise provides a new paradigm and formalism to quantify cross-communication among diverse systems across levels and time scales to improve our understanding of exercise-related phenomena and opens new horizons for exercise testing in health and disease.

Integrative Proposals of Sports Monitoring: Subjective Outperforms Objective Monitoring.

Current trends in sports monitoring are characterized by the massive collection of tech-based biomechanical, physiological and performance data, integrated through mathematical algorithms. However, the application of algorithms, predicated on mechanistic assumptions of how athletes operate, cannot capture, assess and adequately promote athletes' health and performance. The objective of this paper is to reorient the current integrative proposals of sports monitoring by re-conceptualizing athletes as complex adaptive systems (CAS). CAS contain higher-order perceptual units that provide continuous and multilevel integrated information about performer-environment interactions. Such integrative properties offer exceptional possibilities of subjective monitoring for outperforming any objective monitoring system. Future research should investigate how to enhance this human potential to contribute further to athletes' health and performance. This line of argument is not intended to advocate for the elimination of objective assessments, but to highlight the integrative possibilities of subjective monitoring.

Network Physiology of Exercise: Vision and Perspectives.

The basic theoretical assumptions of Exercise Physiology and its research directions, strongly influenced by reductionism, may hamper the full potential of basic science investigations, and various practical applications to sports performance and exercise as medicine. The aim of this perspective and programmatic article is to: (i) revise the current paradigm of Exercise Physiology and related research on the basis of principles and empirical findings in the new emerging field of Network Physiology and Complex Systems Science; (ii) initiate a new area in Exercise and Sport Science, Network Physiology of Exercise (NPE), with focus on basic laws of interactions and principles of coordination and integration among diverse physiological systems across spatio-temporal scales (from the sub-cellular level to the entire organism), to understand how physiological states and functions emerge, and to improve the efficacy of exercise in health and sport performance; and (iii) to create a forum for developing new research methodologies applicable to the new NPE field, to infer and quantify nonlinear dynamic forms of coupling among diverse systems and establish basic principles of coordination and network organization of physiological systems. Here, we present a programmatic approach for future research directions and potential practical applications. By focusing on research efforts to improve the knowledge about nested dynamics of vertical network interactions, and particularly, the horizontal integration of key organ systems during exercise, NPE may enrich Basic Physiology and diverse fields like Exercise and Sports Physiology, Sports Medicine, Sports Rehabilitation, Sport Science or Training Science and improve the understanding of diverse exercise-related phenomena such as sports performance, fatigue, overtraining, or sport injuries.

Theory of Cooperative-Competitive Intelligence: Principles, Research Directions, and Applications.

We present a theory of cooperative-competitive intelligence (CCI), its measures, research program, and applications that stem from it. Within the framework of this theory, satisficing sub-optimal behavior is any behavior that does not promote a decrease in the prospective control of the functional action diversity/unpredictability (D/U) potential of the agent or team. This potential is defined as the entropy measure in multiple, context-dependent dimensions. We define the satisficing interval of behaviors as CCI. In order to manifest itself at individual or team level, this capacity harnesses properties such as degeneracy, pleiotropy (pluri-potentiality), synergies, and metastability. Intelligence is embodied because intelligent behavior is deeply dependent on body functionalities, defined as entropy measures. We base our theory on three principles: (a) relativity of functional entropy/information in agent (team)-environment systems, (b) tendency toward the satisficing level of D/U potential, and (c) tendency toward the non-decreasing D/U potential. The conjunction of these three principles provides existence of sub-optimal behaviors associated with CCI. First, we deal with the problem of how to reduce multidimensional behavior to a concept that accounts for the vast set of scenarios in which CCI is manifested. Secondly, we define and discuss the three interacting principles that underpin CCI behavior as well as providing an outline for a future CCI research program supported by agent-based modeling and empirical research. Finally, we provide some preliminary practical issues that stem from the theory.

Training or Synergizing? Complex Systems Principles Change the Understanding of Sport Processes.

There is a need to update scientific assumptions in sport to promote the critical thinking of scientists, coaches, and practitioners and improve their methodological decisions. On the basis of complex systems science and theories of biological evolution, a systematization and update of theoretical and methodological principles to transform the understanding of sports training is provided. The classical focus on learning/acquiring skills and fitness is replaced by the aim of increasing the diversity/unpredictability potential of teams/athletes through the development of synergies. This development is underpinned by the properties of hierarchical organization and circular causality of constraints, that is, the nestedness of constraints acting at different levels and timescales. These properties, that integrate bottom-up and top-down all dimensions and levels of performance (from social to genetic), apply to all types of sport, ages, or levels of expertise and can be transferred to other fields (e.g., education, health, management). The team as the main training unit of intervention, the dynamic concept of task representativeness, and the co-adaptive and synergic role of the agents are some few practical consequences of moving from training to synergizing.

Interlimb Coordination: A New Order Parameter and a Marker of Fatigue During Quasi-Isometric Exercise?

Although exercise-induced fatigue has been mostly studied from a reductionist and component-dominant approach, some authors have started to test the general predictions of theories of self-organized change during exercises performed until exhaustion. However, little is known about the effects of fatigue on interlimb coordination in quasi-isometric actions. The aim of this study was to investigate the effect of exercise-induced fatigue on upper interlimb coordination during a quasi-isometric exercise performed until exhaustion. In order to do this, we hypothesized an order parameter that governs the interlimb coordination as an interlimb correlation measure. In line with general predictions of theory of phase transitions, we expected that the locally averaged values of the order parameter will increase as the fatigue driven system approaches the point of spontaneous task disengagement. Seven participants performed a quasi-isometric task holding an Olympic bar maintaining an initial elbow flexion of 90 degrees until fatigue induced spontaneous task disengagement. The variability of the elbow angle was recorded through electrogoniometry and the obtained time series were divided into three segments for further analysis. Running correlation function (RCF) and adopted bivariate phase rectified signal averaging (BPRSA) were applied to the corresponding initial (30%) and last (30%) segments of the time series. The results of both analyses showed that the interlimb correlation increased between the initial and the final segments of the performed task. Hence, the hypothesis of the research was supported by evidence. The enhancement of the correlation in the last part means a less flexible coordination among limbs. Our results also show that the high magnitude correlation (%RCF > 0.8) and the %Range (END-BEG) may prove to be useful markers to detect the effects of effort accumulation on interlimb coordination. These results may provide information about the loss of adaptability during exercises performed until exhaustion. Finally, we briefly discuss the hypothesis of the inhibitory percolation process being the general explanation of the spontaneous task disengagement phenomenon.

Effects of Temporary Numerical Imbalances on Collective Exploratory Behavior of Young and Professional Football Players.

The aim of this study was to explore how the use of temporary numerical imbalances during small-sided Game SSGs affects team's exploratory behaviors (i.e., variety and quantity of responses given in an ever-changing game context and its rate of change) in different age groups. Two different age groups (under-15 and under-23) of football players participated in the study. For each age group, three teams of five players played six small-sided games of 5 min duration in different conditions: (i) numerical balance (GK + 4 vs. 4 + GK); (ii) temporary numerical imbalance, which consisted of a numerical change of teammates and opponents every one minute. Latitude and longitude GPS coordinates were used to determine the positioning-derived variables. The dynamic overlap (i.e. the measure of average similarity of the game patterns that take place in increasingly larger time intervals) was used to provide information of the rate and breadth of exploratory behavior. The results revealed that the long-term exploratory breadth increased for the under-23 age group. Non-clear effects were found for the short-term rate of exploration, but with an increasing trend. In the under-15 group, the exploratory behavior was more likely to increase in the long term. The increase for the short-term rate of exploration was unclear, but it follows an increasing trend. These results suggest that the use of temporary numerical imbalances could offer coaches more dynamic training situations and different adaptive training environments similar to matches.

Principal component analysis as a novel approach for cardiorespiratory exercise testing evaluation.

OBJECTIVE: Our purpose was to apply a principal component analysis (PCA) approach to cardiorespiratory exercise to test evaluation and its sensitivity to workload accumulation. APPROACH: Twenty-five healthy young adults performed a progressive and maximal cycling test, which was divided into two parts: moderate and high workload intensities, using a ventilatory threshold as a cut point. A PCA of the time series of cardiovascular and respiratory variables was performed in each part and the number of principal components (PCs), the eigenvalues of the first PC (PC1), and the information entropy were calculated. MAIN RESULTS: The number of PCs increased, the eigenvalues of PC1 decreased (t = 5.32; p  < 0.001; d = 1.39) and entropy was significantly higher (Z = 3.10; p  = .002; d = 1.16) at high workload intensities, compared to moderate intensities. SIGNIFICANCE: Results showed the sensitivity of the PCA approach to workload accumulation and corroborates its potential for improving the evaluation and interpretation of cardiorespiratory exercise testing. In particular, it points to being a good candidate to objectively detect qualitative changes or thresholds.

Physiological- and performance-related effects of acute olive oil supplementation at moderate exercise intensity.

BACKGROUND: The consumption of olive oil is associated with a diminished risk of cardiovascular disorders and mortality, but the impact of olive oil supplementation on endurance performance is still unclear. Since the beneficial effects of olive oil are observed at a systemic level, its effectiveness may not be precisely measured through the commonly registered maximal and threshold values of some physiological and performance parameters. In contrast, we suggest evaluating it through variables able to capture the coordinated behaviour of physiological systems. Thus, the aim of the current research was to assess the effect of an acute extra virgin olive oil supplementation on cardiorespiratory coordination (CRC) and performance, compared to palm oil. METHODS: Three separate effort test sessions were carried out separated by 7-day interval. During each session, participants (n = 7) repeated the same progressive and maximal walking test, but under different dietary supplementations in a randomized order: (1) olive oil, (2) palm oil, and (3) placebo. A principal component (PC) analysis of selected cardiovascular and cardiorespiratory variables was carried out to evaluate CRC. Eigenvalues of the first PC (PC1) and the loadings of the cardiorespiratory variables onto PC1 were compared among dietary supplementations. In order to more accurately evaluate CRC, all the tests were divided into 3 equal sections, corresponding to low, moderate, and high exercise intensities, and the aforementioned procedure was repeated for each section in all the tests. RESULTS: Statistically significant differences were observed regarding PC1 eigenvalues among dietary supplementations (χ2 (8,2) = 6.3; p = .04), only at moderate intensity exercise. Specifically, PC1 eigenvalues were higher under olive oil compared to palm oil (2.63 ± 0.51 vs. 2.30 ± 0.28; Z = 2.03; p = .04; d = 0.80) and placebo supplementations (2.63 ± 0.51 vs. 2.38 ± 0.36; Z = 2.20; p = .03; d = 0.57). CONCLUSIONS: Supplementation with extra virgin olive oil increased CRC during a progressive walking test at moderate intensity, although did not change performance and other physiological markers. CRC analysis appears as a sensitive tool to investigate the physiological and performance effects of dietary supplementations.

On the Relatedness and Nestedness of Constraints.

The purpose of this opinion paper is providing a platform for explaining and discussing the relatedness and nestedness of constraints on the basis of four claims: (a) task constraints are distributed between the person and the environment and hence are relational variables, (b) being relational, task constraints are also emergent properties of the organism/environment system, (c) constraints are nested in timescales, and (d) a vast set of constraints are correlated through circular causality. Theoretical implications for improving the understanding of the constraints-led approach and practical applications for enhancing the manipulation of constraints in learning and training settings are proposed.

Flow as an Embodied State. Informed Awareness of Slackline Walking.

Flow during exercise has been theorized and studied solely through subjective-retrospective methods as a "scull bound" construct. Recent advances of the radical embodied perspectives on conscious mind and cognition pose challenges to such understanding, particularly because flow during exercise is associated with properties of performer's movement behavior. In this paper we use the concept of informed awareness to reconceptualize flow experience as a property of the performer-environment coupling, and study it during a slackline walking task. To empirically check the possible relatedness of the behavior-experience complementary pair, two measures were considered. The experiential realm was quantified by the flow short scale and the behavioral realm by the Hurst (H) exponent obtained through accelerometry time series of the legs and the center of body mass (CoM). In order to obtain a coarse-grained insight about the degree of co-varying within the perception-action flow of performers, we conducted correlational and multiple regression analyses. Measures of behavioral variables (H exponents of the dominant, subdominant leg and the CoM, were treated as explanatory, and the flow scale and its subscale (fluency of movements and absorption) scores asresponse variables containing summarized information about perceptual experiences of performers. In order to check for possible mediating or confounding effects of training parameters on the action-perception variables' covariance, we included two additional variables which measured the degree of engagement of participants with the task. Results revealed that the temporal structure of fluctuations of the dominant leg, as measured by the Hurst exponent, was a strong mediator of effects of training variables and the subdominant leg fluctuations, on the flow scale and the subscale scores. The magnitude of Hurst exponents of both legs was informative about the degree of stability within the performer-environment system. The degree of critical slowing down, as measured by Hurst exponents, consistently co-varied with the flow scale and subscales. The experience of flow during the slackline walking task was dominantly saturated by the perceived fluency of movements and less so by the absorption experience. The stable co-variance of perception-action variables signified the embodied nature of the flow experience.

From microscopic to macroscopic sports injuries. Applying the complex dynamic systems approach to sports medicine: a narrative review.

A better understanding of how sports injuries occur in order to improve their prevention is needed for medical, economic, scientific and sports success reasons. This narrative review aims to explain the mechanisms that underlie the occurrence of sports injuries, and an innovative approach for their prevention on the basis of complex dynamic systems approach. First, we explain the multilevel organisation of living systems and how function of the musculoskeletal system may be impaired. Second, we use both, a constraints approach and a connectivity hypothesis to explain why and how the susceptibility to sports injuries may suddenly increase. Constraints acting at multiple levels and timescales replace the static and linear concept of risk factors, and the connectivity hypothesis brings an understanding of how the accumulation of microinjuries creates a macroscopic non-linear effect, that is, how a common motor action may trigger a severe injury. Finally, a recap of practical examples and challenges for the future illustrates how the complex dynamic systems standpoint, changing the way of thinking about sports injuries, offers innovative ideas for improving sports injury prevention.

Free Play with Certain Equipment Constrains the Emergence of Exploratory Behavior and Physical Activity in Preschoolers.

The aim of this study was to establish whether various types of play equipment differentially constrain exploratory behavior and spontaneous physical activity patterns in preschoolers when they play freely with them. Fourteen children, 3-4 years old, played with four different types of equipment. The children wore an accelerometer and data were analyzed taking into account overall physical activity, the time spent at different intensity levels of move-ment, and the entropy of the time series. All trials were video-recorded and a systematic observation instrument was used to register actions and interactions with partners and equipment. The data were subsequently analyzed by means of principal component analysis (PCA) and the dynamic overlap order parameter, to identify the rate and breadth of exploratory behavior on different time scales. Our results revealed that the children were physically less active when playing with portable and small equipment. The PCA showed that when playing without equipment, the children usually played standardized games, like playing tag, in groups. Movement patterns were more or less varied depending on the equip-ment, and thus some equipment constrained the emergence of different types of games or motor behavior.

Dynamics of tactical behaviour in association football when manipulating players' space of interaction.

The analysis of positional data in association football allows the spatial distribution of players during matches to be described in order to improve the understanding of tactical-related constraints on the behavioural dynamics of players. The aim of this study was to identify how players' spatial restrictions affected the exploratory tactical behaviour and constrained the perceptual-motor workspace of players in possession of the ball, as well as inter-player passing interactions. Nineteen professional outfield male players were divided into two teams of 10 and 9 players, respectively. The game was played under three spatial constraints: a) players were not allowed to move out of their allocated zones, except for the player in possession of the ball; b) players were allowed to move to an adjacent zone, and; c) non-specific spatial constraints. Positional data was captured using a 5 Hz interpolated GPS tracking system and used to define the configuration states of players for each second in time. The configuration state comprised 37 categories derived from tactical actions, distance from the nearest opponent, distance from the target and movement speed. Notational analysis of players in possession of the ball allowed the mean time of ball possession and the probabilities of passing the ball between players to be calculated. The results revealed that the players' long-term exploratory behaviour decreased and their short-term exploration increased when restricting their space of interaction. Relaxing players' positional constraints seemed to increase the speed of ball flow dynamics. Allowing players to move to an adjacent sub-area increased the probabilities of interaction with the full-back during play build-up. The instability of the coordinative state defined by being free from opponents when players had the ball possession was an invariant feature under all three task constraints. By allowing players to move to adjacent sub-areas, the coordinative state became highly unstable when the distance from the target decreased. Ball location relative to the scoring zone and interpersonal distance constitute key environmental information that constrains the players' coordinative behaviour. Based on our results, dynamic overlap is presented as a good option to capture tactical performance. Moreover, the selected collective (i.e. relational) variables would allow coaches to identify the effects of training drills on teams and players' behaviour. More research is needed considering these type variables to understand how the manipulation of constraints induce a more stable or flexible dynamical structure of tactical behaviour.

Cardiorespiratory Coordination in Repeated Maximal Exercise.

Increases in cardiorespiratory coordination (CRC) after training with no differences in performance and physiological variables have recently been reported using a principal component analysis approach. However, no research has yet evaluated the short-term effects of exercise on CRC. The aim of this study was to delineate the behavior of CRC under different physiological initial conditions produced by repeated maximal exercises. Fifteen participants performed 2 consecutive graded and maximal cycling tests. Test 1 was performed without any previous exercise, and Test 2 6 min after Test 1. Both tests started at 0 W and the workload was increased by 25 W/min in males and 20 W/min in females, until they were not able to maintain the prescribed cycling frequency of 70 rpm for more than 5 consecutive seconds. A principal component (PC) analysis of selected cardiovascular and cardiorespiratory variables (expired fraction of O2, expired fraction of CO2, ventilation, systolic blood pressure, diastolic blood pressure, and heart rate) was performed to evaluate the CRC defined by the number of PCs in both tests. In order to quantify the degree of coordination, the information entropy was calculated and the eigenvalues of the first PC (PC1) were compared between tests. Although no significant differences were found between the tests with respect to the performed maximal workload (Wmax), maximal oxygen consumption (VO2 max), or ventilatory threshold (VT), an increase in the number of PCs and/or a decrease of eigenvalues of PC1 (t = 2.95; p = 0.01; d = 1.08) was found in Test 2 compared to Test 1. Moreover, entropy was significantly higher (Z = 2.33; p = 0.02; d = 1.43) in the last test. In conclusion, despite the fact that no significant differences were observed in the conventionally explored maximal performance and physiological variables (Wmax, VO2 max, and VT) between tests, a reduction of CRC was observed in Test 2. These results emphasize the interest of CRC evaluation in the assessment and interpretation of cardiorespiratory exercise testing.

Structure and dynamics of European sports science textual contents: Analysis of ECSS abstracts (1996-2014).

The article discusses general structure and dynamics of the sports science research content as obtained from the analysis of 21998 European College of Sport Science abstracts belonging to 12 science topics. The structural analysis showed intertwined multidisciplinary and unifying tendencies structured along horizontal (scope) and vertical (level) axes. Methodological (instrumental and mode of inquiry) integrative tendencies are dominant. Theoretical integrative tendencies are much less detectable along both horizontal and vertical axes. The dynamic analysis of written abstracts text content over the 19 years reveals the contextualizing and guiding role of thematic skeletons of each sports science topic in forming more detailed contingent research ideas and the role of the latter in stabilizing and procreating the former. This circular causality between both hierarchical levels and functioning on separate characteristic time scales is crucial for understanding how stable research traditions self-maintain and self-procreate through innovative contingencies. The structure of sports science continuously rebuilds itself through use and re-use of contingent research ideas. The thematic skeleton ensures its identity and the contingent conceptual sets its flexibility and adaptability to different research or applicative problems.