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1.
Dev Comp Immunol ; 53(1): 85-95, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26112000

RESUMO

White spot syndrome virus (WSSV), the causative agent of white spot disease (WSD), is a serious and aggressive shrimp viral pathogen with a worldwide distribution. At the genome replication stage (12 hpi), WSSV induces a metabolic rerouting known as the invertebrate Warburg effect, which boosts the availability of energy and biosynthetic building blocks in the host cell. Here we show that unlike the lipogenesis that is seen in cancer cells that are undergoing the Warburg effect, at 12 hpi, all of the long chain fatty acids (LCFAs) were significantly decreased in the stomach cells of WSSV-infected shrimp. By means of this non-selective WSSV-induced lipolysis, the LCFAs were apparently diverted into ß-oxidation and used to replenish the TCA cycle. Conversely, at 24 hpi, when the Warburg effect had ceased, most of the LCFAs were significantly up-regulated and the composition was also significantly altered. In crayfish these changes were in a direction that appeared to favor the formation of WSSV virion particles. We also found that, at 24 hpi, but not at 12 hpi, the PI3K-Akt-mTOR-HIF1α pathway induced the expression of fatty acid synthase (FAS), an enzyme which catalyzes the conversion of acetyl-CoA into LCFAs. WSSV virion formation was impaired in the presence of the FAS inhibitor C75, although viral gene and viral DNA levels were unaffected. WSSV therefore appears to use the PI3K-Akt-mTOR pathway to induce lipid biosynthesis at 24 hpi in order to support viral morphogenesis.


Assuntos
Metabolismo Energético/fisiologia , Ácidos Graxos/biossíntese , Ácidos Graxos/metabolismo , Penaeidae/virologia , Vírus da Síndrome da Mancha Branca 1/metabolismo , 4-Butirolactona/análogos & derivados , 4-Butirolactona/farmacologia , Acetilcoenzima A/metabolismo , Animais , Ácido Graxo Sintases/antagonistas & inibidores , Ácido Graxo Sintases/biossíntese , Ácidos Graxos/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Lipólise/fisiologia , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo
2.
PLoS Pathog ; 10(6): e1004196, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24945378

RESUMO

In this study, we used a systems biology approach to investigate changes in the proteome and metabolome of shrimp hemocytes infected by the invertebrate virus WSSV (white spot syndrome virus) at the viral genome replication stage (12 hpi) and the late stage (24 hpi). At 12 hpi, but not at 24 hpi, there was significant up-regulation of the markers of several metabolic pathways associated with the vertebrate Warburg effect (or aerobic glycolysis), including glycolysis, the pentose phosphate pathway, nucleotide biosynthesis, glutaminolysis and amino acid biosynthesis. We show that the PI3K-Akt-mTOR pathway was of central importance in triggering this WSSV-induced Warburg effect. Although dsRNA silencing of the mTORC1 activator Rheb had only a relatively minor impact on WSSV replication, in vivo chemical inhibition of Akt, mTORC1 and mTORC2 suppressed the WSSV-induced Warburg effect and reduced both WSSV gene expression and viral genome replication. When the Warburg effect was suppressed by pretreatment with the mTOR inhibitor Torin 1, even the subsequent up-regulation of the TCA cycle was insufficient to satisfy the virus's requirements for energy and macromolecular precursors. The WSSV-induced Warburg effect therefore appears to be essential for successful viral replication.


Assuntos
Penaeidae/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Vírus da Síndrome da Mancha Branca 1/genética , Aminoácidos/biossíntese , Aminoácidos/metabolismo , Animais , Ciclo do Ácido Cítrico/genética , Metabolismo Energético/genética , Glicólise/genética , Alvo Mecanístico do Complexo 1 de Rapamicina , Alvo Mecanístico do Complexo 2 de Rapamicina , Metaboloma/genética , Proteínas Monoméricas de Ligação ao GTP/genética , Complexos Multiproteicos/antagonistas & inibidores , Complexos Multiproteicos/genética , Naftiridinas/farmacologia , Penaeidae/virologia , Via de Pentose Fosfato/genética , Proteoma/genética , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Serina-Treonina Quinases TOR/antagonistas & inibidores , Serina-Treonina Quinases TOR/genética , Replicação Viral/genética , Vírus da Síndrome da Mancha Branca 1/metabolismo
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