RESUMO
Homodimers are the most common type of protein assembly in nature and have distinct features compared with heterodimers and higher order oligomers. Understanding homodimer interactions at the atomic level is critical both for elucidating their biological mechanisms of action and for accurate modeling of complexes of unknown structure. Computation-based design of novel protein-protein interfaces can serve as a bottom-up method to further our understanding of protein interactions. Previous studies have demonstrated that the de novo design of homodimers can be achieved to atomic-level accuracy by ß-strand assembly or through metal-mediated interactions. Here, we report the design and experimental characterization of a α-helix-mediated homodimer with C2 symmetry based on a monomeric Drosophila engrailed homeodomain scaffold. A solution NMR structure shows that the homodimer exhibits parallel helical packing similar to the design model. Because the mutations leading to dimer formation resulted in poor thermostability of the system, design success was facilitated by the introduction of independent thermostabilizing mutations into the scaffold. This two-step design approach, function and stabilization, is likely to be generally applicable, especially if the desired scaffold is of low thermostability.
Assuntos
Dimerização , Engenharia de Proteínas/métodos , Sequência de Aminoácidos , Animais , Dicroísmo Circular , Cristalografia por Raios X , Proteínas de Drosophila , Proteínas de Homeodomínio/química , Temperatura Alta , Modelos Moleculares , Simulação de Acoplamento Molecular , Dados de Sequência Molecular , Mutação , Ressonância Magnética Nuclear Biomolecular , Conformação Proteica , Mapeamento de Interação de Proteínas , Estabilidade Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Soluções , Fatores de Transcrição/químicaRESUMO
Many proteins are induced in the plant defense response to biotic stress or mechanical wounding. One group is lectins. Ipomoelin (IPO) is one of the wound-inducible proteins of sweet potato (Ipomoea batatas cv. Tainung 57) and is a Jacalin-related lectin (JRL). In this study, we resolved the crystal structures of IPO in its apo form and in complex with carbohydrates such as methyl α-D-mannopyranoside (Me-Man), methyl α-D-glucopyranoside (Me-Glc), and methyl α-D-galactopyranoside (Me-Gal) in different space groups. The packing diagrams indicated that IPO might represent a compact tetrameric association in the JRL family. The protomer of IPO showed a canonical ß-prism fold with 12 strands of ß-sheets but with 2 additional short ß-strands at the N terminus. A truncated IPO (ΔN10IPO) by removing the 2 short ß-strands of the N terminus was used to reveal its role in a tetrameric association. Gel filtration chromatography confirmed IPO as a tetrameric form in solution. Isothermal titration calorimetry determined the binding constants (K(A)) of IPO and ΔN10IPO against various carbohydrates. IPO could bind to Me-Man, Me-Glc, and Me-Gal with similar binding constants. In contrast, ΔN10IPO showed high binding ability to Me-Man and Me-Glc but could not bind to Me-Gal. Our structural and functional analysis of IPO revealed that its compact tetrameric association and carbohydrate binding polyspecificity could be regulated by the 2 additional N-terminal ß-strands. The versatile carbohydrate binding properties of IPO might play a role in plant defense.
Assuntos
Metabolismo dos Carboidratos , Lectinas de Plantas/química , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Sítios de Ligação , Sequência de Carboidratos , Carboidratos/química , Cromatografia em Gel , Cristalografia por Raios X , Ligação de Hidrogênio , Cinética , Metilação , Modelos Moleculares , Dados de Sequência Molecular , Proteínas Mutantes/metabolismo , Ligação Proteica , Estrutura Quaternária de Proteína , Alinhamento de Sequência , Relação Estrutura-AtividadeRESUMO
Adenosylcobalamin (AdoCbl)-dependent glutamate mutase from Clostridium tetanomorphum comprises two weakly-associating subunits, MutS and MutE, which combine with AdoCbl to form the active holo-enzyme. Three coenzyme analogs, methylcobinamide (MeCbi), adenosylcobinamide (AdoCbi) and adeosylcobinamide-GDP (AdoCbi-GDP), were synthesized at milligram scale. Equilibrium dialysis was used to measure the binding of coenzyme B(12) analogs to glutamate mutase. Our results show that, unlike AdoCbl-dependent methylmalonyl CoA mutase, the ratio k(cat)/K(m) decreased approximately 10(4)-fold in both cases when AdoCbi or AdoCbi-GDP was used as the cofactor. The coenzyme analog-binding studies show that, in the absence of the ribonucleotide tail of AdoCbl, the enzyme's active site cannot correctly accommodate the coenzyme analog AdoCbi. The results presented here shed some light on the cobalt-carbon cleavage mechanism of B(12).
Assuntos
Proteínas de Bactérias/química , Clostridium tetanomorphum/enzimologia , Cobamidas/química , Transferases Intramoleculares/química , Proteínas de Bactérias/metabolismo , Catálise , Cobamidas/síntese química , Cobamidas/metabolismo , Diálise , Transferases Intramoleculares/metabolismo , Cinética , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Ligação Proteica , Espectrofotometria , Espectrofotometria Ultravioleta , TermodinâmicaRESUMO
The bi-functional enzyme, adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU), is involved in the biosynthesis of cobalamin in Salmonella typhimurium, and, therefore, can be used for the in vitro synthesis of analogs of B(12). Previously, five different steps were required to purify the recombinant enzyme from Escherichia coli. Here, we describe the cloning, sequencing, and expression of the cobU gene from S. typhimurium ATCC 19585 and, without introducing a purification tag sequence to the N- or C-terminus of the recombinant enzyme, a new single-step purification method based on hydrophobic interaction chromatography.
