Deep learning based coherent diffraction imaging of dynamic scattering media.

The ptychographic iterative engine (PIE) is a lensless coherent diffraction imaging algorithm known for its simplicity, easy to use, scalability, and fast convergence. However, practical applications often encounter interference in imaging results caused by non-static scattering media, such as dense fog, seawater target detection and medical biology diagnosis. To address this challenge, we propose a novel approach using computational deep learning for dynamic scattering medium image reconstruction, enabling lens-free coherent diffraction imaging through dynamic scattering media. Through extensive analysis, we evaluate the effectiveness of the neural network for PIE image recovery under varying scattering medium concentration conditions. We also test scattering images obtained by hybrid training with different concentrations of scattering medium to assess the generalisation ability of the neural network. The experimental results demonstrate that our proposed method achieve PIE lens-free imaging under non-static scattering media interference. This coherent diffraction imaging method, based on transmission through dynamic scattering media, opens up new possibilities for practical applications of PIE and fosters its development in complex environments. Its significance extends to fields like atmospheric pollution, seawater target detection and medical biology diagnosis, providing valuable references for research in these domains.

Enhancement of antiproliferative and proapoptotic effects of cadmium chloride combined with hSmac in hepatocellular carcinoma cells.

BACKGROUND: To study the effects of cadmium chloride (CdCl(2)) combined with hSmac on the proliferation and apoptosis of hepatocellular carcinoma cells, i.e. SMMC-7721. METHODS: SMMC-7721 cells were transfected with pcDNA3.1(+)-hSmac using a lipofectamine-mediated method, and then cell viability was detected by MTT assay after exposure to 10, 20, and 30 µmol/l CdCl(2). Apoptosis was determined by both acridine orange-ethidium bromide staining and flow cytometry, and expressions of caspase-3, caspase-9, and cytochrome c by Western blot. RESULTS: CdCl(2) had cytotoxicity to SMMC-7721 cells, and it could inhibit proliferation in a dose-dependent manner and induce apoptosis; hSmac could inhibit proliferation and induce apoptosis independently in SMMC-7721 cells. Furthermore, cotreatment with CdCl(2) and hSmac could enhance antiproliferative and proapoptotic effects in SMMC-7721 cells. CONCLUSIONS: hSmac could enhance the cytotoxicity of CdCl(2).

Mercury distribution in neonatal rat brain after intrauterine methylmercury exposure.

Methylmercuric chloride (MMC) was orally administered to pregnant Wistar rats from gestational day 6 (G6) for 5 consecutive days. After delivery, the neonatal rats were decapitated and the cerebrum, cerebellum and hippocampus were excised on postnatal day (PND) 1, 7, 14, 21, 30 to determine total Hg contents and concentrations (six per stage). Both total Hg contents and concentrations in all the three regions increased as exposure dose increased and declined as postnatal time prolonged. Interestingly, differences of total Hg content between cerebrum and hippocampus at each time-point were significant (P<0.05). In the meantime, considering the Hg concentration, while no differences were observed before PND14 (P<0.05) among the three regions, Hg concentration in hippocampus was significantly higher than in cerebrum after that time period (P<0.05). We demonstrated that MeHg could pass through the placental and blood-brain barriers in a dose-dependent manner. Moreover, we found mercury redistribution occurred in offspring brain following the prolongation of postnatal time. The hippocampus was the major target of MeHg accumulation.