RESUMO
AIM: To explore the effect of Jagged1 on the morphology of dendritic cell(DC) differentiation. METHODS: Mouse bone marrow cells were cultured in the presence of rmIL-4 and rmGM-CSF. Atomic force microscopy (AFM) was used to observe the ultrastructure of these DC. RESULTS: Similar to the control, the cells in Jagged1 group displayed irregular shape with spiny or sheet-like dendrites and the cellular surface was rough. In contrast, the cells in DAPT group exhibited little dendritic processes and most of them were of round shape and with smooth surface. Furthermore, the height of the cells in Jagged1 group was higher than that in control and DAPT group, while the diameter of the cells in both was roughly equivalent. CONCLUSION: Jagged1 has no significant effect on the morphology of DC, but significant changes in the morphology of DC in DAPT group have occurred.
