Grevillosides R-S: glucosides of 5-alkylresorcinol derivatives from roots of Ardisia crispa (Thunb.) A. DC.

Grevillosides R-S (1-2), two new glucosides of 5-alkylresorcinol derivatives, were isolated from the roots of Ardisia crispa (Thunb.) A. DC. The structures of grevillosides R-S (1-2) were determined by 1D and 2D NMR, HR-MS, UV, IR experiments and by comparison of their spectroscopic and physical data with literature values. In this paper, grevillosides R-S (1-2) were tested for their radical-scavenging activity (DPPH and ABTS) and α-glucosidase inhibitory activity in vitro. Grevillosides R-S (1-2) exhibited weak DPPH radical-scavenging activity with IC50 values of 72.3 and 485.2 µM, respectively. Grevillosides R-S (1-2) exhibited no inhibitory activity against α-glucosidase.

Terpenoids with Cytotoxic Activity from Roots of Ardisia crispa.

Three new terpenoids, ardisiacrispins G-I (1, 4 and 8), and eight known compounds, cyclamiretin A (2), psychotrianoside G (3), 3-hydroxy-ß-damascone (5), megastigmane (6), corchoionol C (7), zingiberoside B (9), angelicoidenol (10), trans-linalool-3,6-oxide-ß-D-glucopyranoside (11) were isolated from the roots of Ardisia crispa. The chemical structures of all isolated compounds were elucidated by extensive spectroscopic analyses, such as HR-ESI-MS, 1D and 2D NMR spectra. Ardisiacrispin G (1) represents the oleanolic-type scaffold featuring a rare 15,16- epoxy system. All compounds were evaluated for the cytotoxicity against two cancer cell lines (U87 MG and HepG2) in vitro. Compounds 1, 8 and 9 exhibited moderate cytotoxic activity with IC50 values ranging from 7.6±1.1 to 28.8±3.2 µM.

Two new amides from the seeds of Coix lacryma-jobi var. lacryma-jobi.

Phytochemical investigation of the seeds of Coix lacryma-jobi var. lacryma-jobi resulted in the isolation of eight compounds, including two new structures, coixdines A-B (1-2) and six known compounds (3-8). The structures of these compounds were determined by 1 D and 2 D NMR spectra referring to the literatures, together with HR-MS analysis. Coixdine A and B are first examples of spermidine skeleton in genus Coix. In the present paper, all compounds were evaluated for the cytotoxicity against two cancer cell lines (CT-26 and BxPC-3) in vitro, and none of the compounds exhibited obvious cytotoxic activity. The present investigation suggests that these amides seem to be of great chemotaxonomic value for C. lacryma-jobi.