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1.
Sensors (Basel) ; 24(6)2024 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-38544042

RESUMO

In order to effectively respond to floods and water emergencies that result in the drowning of missing persons, timely and effective search and rescue is a very critical step in underwater rescue. Due to the complex underwater environment and low visibility, unmanned underwater vehicles (UUVs) with sonar are more efficient than traditional manual search and rescue methods to conduct active searches using deep learning algorithms. In this paper, we constructed a sound-based rescue target dataset that encompasses both the source and target domains using deep transfer learning techniques. For the underwater acoustic rescue target detection of small targets, which lack image feature accuracy, this paper proposes a two-branch convolution module and improves the YOLOv5s algorithm model to design an acoustic rescue small target detection algorithm model. For an underwater rescue target dataset based on acoustic images with a small sample acoustic dataset, a direct fine-tuning using optical image pre-training lacks cross-domain adaptability due to the different statistical properties of optical and acoustic images. This paper therefore proposes a heterogeneous information hierarchical migration learning method. For the false detection of acoustic rescue targets in a complex underwater background, the network layer is frozen during the hierarchical migration of heterogeneous information to improve the detection accuracy. In addition, in order to be more applicable to the embedded devices carried by underwater UAVs, an underwater acoustic rescue target detection algorithm based on ShuffleNetv2 is proposed to improve the two-branch convolutional module and the backbone network of YOLOv5s algorithm, and to create a lightweight model based on hierarchical migration of heterogeneous information. Through extensive comparative experiments conducted on various acoustic images, we have thoroughly validated the feasibility and effectiveness of our method. Our approach has demonstrated state-of-the-art performance in underwater search and rescue target detection tasks.

2.
Sensors (Basel) ; 19(10)2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31126023

RESUMO

Human posture and movement analysis is important in the areas of rehabilitation, sports medicine, and virtual training. However, the development of sensors with good accuracy, low cost, light weight, and suitability for long durations of human motion capture is still an ongoing issue. In this paper, a new flexible textile sensor for knee joint movement measurements was developed by using ordinary fabrics and conductive yarns. An electrogoniometer was adopted as a standard reference to calibrate the proposed sensor and validate its accuracy. The knee movements of different daily activities were performed to evaluate the performance of the sensor. The results show that the proposed sensor could be used to monitor knee joint motion in everyday life with acceptable accuracy.


Assuntos
Movimento/fisiologia , Dispositivos Eletrônicos Vestíveis , Atividades Cotidianas , Humanos , Articulação do Joelho/fisiologia , Têxteis
3.
EMBO J ; 31(21): 4165-78, 2012 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-23010778

RESUMO

The contribution of human subtelomeric DNA and chromatin organization to telomere integrity and chromosome end protection is not yet understood in molecular detail. Here, we show by ChIP-Seq that most human subtelomeres contain a CTCF- and cohesin-binding site within ∼1-2 kb of the TTAGGG repeat tract and adjacent to a CpG-islands implicated in TERRA transcription control. ChIP-Seq also revealed that RNA polymerase II (RNAPII) was enriched at sites adjacent to the CTCF sites and extending towards the telomere repeat tracts. Mutation of CTCF-binding sites in plasmid-borne promoters reduced transcriptional activity in an orientation-dependent manner. Depletion of CTCF by shRNA led to a decrease in TERRA transcription, and a loss of cohesin and RNAPII binding to the subtelomeres. Depletion of either CTCF or cohesin subunit Rad21 caused telomere-induced DNA damage foci (TIF) formation, and destabilized TRF1 and TRF2 binding to the TTAGGG proximal subtelomere DNA. These findings indicate that CTCF and cohesin are integral components of most human subtelomeres, and important for the regulation of TERRA transcription and telomere end protection.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Cromatina/genética , Proteínas Cromossômicas não Histona/metabolismo , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Proteínas Repressoras/metabolismo , Telômero/genética , Fatores de Transcrição/genética , Transcrição Gênica , Fator de Ligação a CCCTC , Proteínas de Ciclo Celular/genética , Células Cultivadas , Imunoprecipitação da Cromatina , Proteínas Cromossômicas não Histona/genética , Ilhas de CpG/genética , Ensaio de Desvio de Mobilidade Eletroforética , Imunofluorescência , Humanos , Luciferases/metabolismo , Neoplasias/genética , Neoplasias/patologia , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Regiões Promotoras Genéticas/genética , RNA Polimerase II/genética , RNA Polimerase II/metabolismo , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Repressoras/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Coesinas
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