Aluminum-based ozone catalysts prepared by mixing method: Characteristics, performance and carbon emissions.

Green and low carbon is an essential direction for the development of water treatment technology. Ozone catalysts prepared by the mixing method have advantages in terms of energy consumption and CO2 emissions, but are considered to be insufficient in catalytic efficiency and stability. In this paper, an Mn-Cu-Ce/Al2O3 (MCCA) catalyst was prepared by optimizing the preparation conditions of the mixing method and the types and ratios of active components. Taking petrochemical secondary effluent (PCSE) as the treatment object, the performance of the catalyst and the carbon emission in the preparation process were studied; and compared with the impregnation method. Results showed that compared with catalysts loaded with other components, the MCCA had a higher removal efficiency for TOC (43.04%) and COD (53.18%), which was basically equivalent to the impregnation method, and the treated effluent reached the expected concentration. MCCA promoted the decomposition rate of O3 by ten times, and the main active species generated were found to be •OH and 1O. Similar to the catalytic ozonation by the catalyst prepared by the impregnation method, the adsorption sites and surface hydroxyl groups on the MCCA surface play a significant role in the degradation of pollutants. However, the carbon emission in the catalyst preparation process of the mixing method was 418.68 kg/ton, which was only 44% of the impregnation method (949.67 kg/ton). Under the global low-carbon transition, this study shows that the mixing method aligns more with the concept of green, clean, and efficient ozone catalyst preparation.

A tailored and rapid approach for ozonation catalyst design.

Catalytic ozonation is widely employed in advanced wastewater treatment owing to its high mineralization of refractory organics. The key to high mineralization is the compatibility between catalyst formulation and wastewater quality. Machine learning can greatly improve experimental efficiency, while fluorescence data can provide additional wastewater quality information on the composition and concentration of organics, which is conducive to optimizing catalyst formulation. In this study, machine learning combined with fluorescence spectroscopy was applied to develop ozonation catalysts (Mn/γ-Al2O3 catalyst was used as an example). Based on the data collected from 52 different catalysts, a machine-learning model was established to predict catalyst performance. The correlation coefficient between the experimental and model-predicted values was 0.9659, demonstrating the robustness and good generalization ability of the model. The range of the catalyst formulations was preliminarily screened by fluorescence spectroscopy. When the wastewater was dominated by tryptophan-like and soluble microbial products, the impregnation concentration and time of Mn(NO3)2 were less than 0.3 mol L-1 and 10 h, respectively. Furthermore, the optimized Mn/γ-Al2O3 formulation obtained by the model was impregnation with 0.155 mol L-1 Mn(NO3)2 solution for 8.5 h and calcination at 600 °C for 3.5 h. The model-predicted and experimental values for total organic carbon removal were 54.48% and 53.96%, respectively. Finally, the improved catalytic performance was attributed to the synergistic effect of oxidation (•OH and 1O2) and the Mn/γ-Al2O3 catalyst. This study provides a rapid approach to catalyst design based on the characteristics of wastewater quality using machine learning combined with fluorescence spectroscopy.

[Para-Bombay phenotype due to bi-allelic heterozygous base deletions of FUT1 gene].

OBJECTIVE: To explore the genetic mechanism underlying a case with para-Bombay phenotype. METHODS: The ABO and Lewis phenotype were identified with serological methods. The coding regions of exons 6 and 7 of the ABO and FUT1 genes were amplified with PCR and directly sequenced. Haploid sequence analysis was carried out on the variant sites of the FUT1 gene. RESULTS: Serological analysis confirmed that the proband has a rare para-Bombay phenotype. Direct sequencing revealed that he was a B.01/O.01.02 heterozygote for the ABO gene, and had heterozygous deletion for the 768 and 881-882 sites of the FUT1 gene. Further haploid analysis showed that the c.881_882delTT deletion has occurred in one haploid while c.768delC was present in the other haploid. The proband was therefore determined as a FUT1*01N.13/01N.20 heterozygote, which have resulted in frameshift in polypeptide chain p.Phe294Cysfs*40 and p.Val257Phefs*23, respectively. CONCLUSION: A rare bi-allelic heterozygous deletion of para-Bombay phenotype has been identified in a blood donor. The c.881_882delTT and c.768delC deletions may decrease the activity of α-1,2-fucosyltransferase.

A case of hyperhaemolysis syndrome in a pregnant Chinese woman with ß-thalassemia during perinatal transfusion.

OBJECTIVES: To report a case of hyperhaemolysis syndrome (HHS) that occurred during perinatal blood transfusion in a pregnant Chinese woman with ß-thalassemia to deepen the understanding of HHS and the risk of transfusion therapy for patients with thalassemia. BACKGROUND: Most HHS cases occur in people with sickle cell disease. So far, no cases of HHS have been reported in the Chinese population. Here, we report a pregnant Chinese women with ß-thalassemia experiencing HHS. METHODS: The patient received ABO- and RhD-matched red blood cell transfusion from six blood donors in four perinatal transfusions. Haemoglobinuria and lower haemoglobin levels compared to those before transfusion were observed after each transfusion, and the lactate dehydrogenase was consistently elevated. The blood samples were collected at different time points during the hospitalisation for direct antiglobulin test (DAT), antibody screening test and acid elution test. The antigens of six blood donors were identified, and the cross-matching tests were repeated using the blood sample of the patient with specific irregular antibodies after the last transfusion. RESULTS: The DAT of the patient was negative for anti-IgG and positive (1+) for anti-C3d, and no red blood cell antibodies were detected in the eluent before, between and after transfusions. Before and between transfusions, blood samples were negative for red blood cell irregular antibodies, whereas IgM anti-P1 and IgG anti-Jka were detected in blood samples the next day after the last transfusion. In the six donors, two were negative for P1 and Jka , one was positive for P1 and negative for Jka , and three were negative for P1 and positive for Jka . The tentative cross-matching tests using the indirect antiglobulin method in saline showed that only agglutination occurred in the blood samples of the patient collected after last transfusion and the three Jka -positive blood donors. DISCUSSION: The clinical manifestations and laboratory test results suggested that HHS occurred in this patient with ß-thalassemia after each transfusion. Clinicians should be aware that HHS can occur with compatible blood transfusion.