Remote focusing optical tweezers for 3D imaging.

We present a remote focusing optical tweezer utilizing a 4f symmetrical optical system to compensate the high-order aberration during annular light refocusing. The position of the optical trap can be adjusted beyond the range of one hundred micrometers in the axial direction by means of tuning the position of the mirror placed in the focal region of the illumination objective lens. This optical tweezer can be combined with a sectioning microscope to realize three-dimensional (3D) imaging, e.g., a confocal microscope using a single water immersion objective lens. All optical elements are placed in one side of the sample, which is very useful for application in fields such as radiation biology, where radiation or magnetism disturbance must be introduced on the other side of the sample. In the experiment, a 10 µm diameter silicon dioxide microsphere and pollen cells immersed in the water are translated along the axis using the optical tweezer and, meanwhile, the sectioning images are obtained using the confocal microscope.

High-resolution dark-field confocal microscopy based on radially polarized illumination.

Dark-field confocal microscopy (DFCM) facilitates the 3D detection and localization of surface and subsurface defects in high-precision optical components. The spatial resolution of conventional DFCM is commonly undermined owing to complementary aperture detection. We employed a radially polarized (RP) beam for illumination in DFCM. The RP beam creates a sub-diffraction-sized longitudinal optical component after being focused and effectively enhances the lateral resolution by 30.33% from 610 nm to 425 nm. The resolution improvement was verified by imaging a 2D sample containing sparsely distributed gold nanorods along with a 3D neodymium glass containing surface and subsurface defects.

Polarization separation assisted optomechanical time-domain analysis with submeter resolution.

Optomechanical time-domain analysis (OMTDA) is a novel approach to measure distributed acoustic impedance of surrounding media with a high spatial resolution based on coherent forward stimulated Brillouin scattering probing. However, the spatial resolution is still limited by the polarization noise and influence of activation pulse. In this Letter, we propose a polarization separation based OMTDA to further improve the resolution. By isolating the acoustic activation and probing process in orthogonal polarization states, the backward Brillouin scattering of an activation pulse is effectively suppressed. Accompanied with the reduced polarization fluctuation brought by polarization-maintaining fiber, a spatial resolution of 0.8 m is experimentally demonstrated over a 34-m-long fiber and the precise distinction between air and alcohol is realized.

Compact dark-field confocal microscopy based on an annular beam with orbital angular momentum.

This study proposes a dark-field confocal microscopy (DFCM) based on fiber-mode excitation-assisted orbital angular momentum annular beam generation with a two-mode fiber to avoid diffraction distortion. The application of optical fibers compresses the DFCM volume, providing new ideas for system miniaturization. System adjustment difficulty is reduced and feasibility is verified by imaging 2D and 3D samples. High imaging contrast is achieved by fully blocking the reflected light and annular illumination with a steady central dark-spot within a propagation distance of 3 m. The application of our scheme can be further extended to detect subsurface defects in optical components and high-contrast biological imaging.