RESUMO
【Objective】 To compare the quantitative detection results of two domestic quantitative real-time PCR reagents in HBV-DNA detection. 【Methods】 A total of 306 serum samples form hepatitis B patients were selected for quantitative parallel detection of high-sensitiveity HBV-DNA using domestic reagent A and B, and the difference and consistency of the results were analyzed. 【Results】 1) The yielding rate of reagent A and B was 86.92% and 84.64%, respectively. The regression linear equation was Y=0.984 9x+ 0.154 9, R2=0.945 7, the correlation coefficient r=0.972 5, indicating the results by the two reagents had good correlation. 2) When the concentration was in the range of(20~1 000) IU/mL, the yielding rates of reagent A and B were 39.9% and 37.6%, respectively. 【Conclusion】 Reagent A is more suitable for post-treatment monitoring in patients with OBI and HBeAg(-), but also can be used to detect HBV pathogens in patients before operations or blood transfusions.
RESUMO
Objective To observe the effect and safety of emergent intracoronary stenting in acute myocardial infarction (AMI) complicated by acute pump failure.Methods Fifteen patients with anterier wall AMI and apparent signs of cardiac shock were (70.1?8.4) years old on average. Their mean blood pressures were less than 85/60 mm?Hg and the ejection fractions (EF) were less than 42% on echocardiography. All the cases were performed in plantation of stenting in left anterior discending branch (LAD), among which, 4 were in right anterior artery (RCA) and 3 in left circumflex (LCX). Before and after stenting, the changes of the patients′ blood pressures, heart rates and cardiac functions tested by ultrasonography were observed. Results In 15 patients, whose LADs were completely occlusive, after stenting, the coronary arteries gained perfect reperfusion. Compared before and after stenting, the blood pressures were increased significantly after stenting [SBP (106?11.8) vs (76.2?4.9)mm?Hg, P
