RESUMO
Drought, one of the most acute abiotic stressors plants encountered, can adversely affect plants growth and development. The fast adjustment of stomatal aperture is necessary for effective drought tolerance in plants. Anion channels were identified as important controllers of stomatal closing via mediating anion efflux. The present study reports the isolation and identification of a SLAC (SLOW ANION CHANNEL-ASSOCIATED 1) ortholog from an ancient desert shrub Ammopiptanthus mongolicus (Maxim.) Cheng f. (AmSLAC1), which is functionally conserved for ABA and drought induced stomata closure. AmSLAC1 was primarily expressed in shoots, especially in guard cells. The transcription of AmSLAC1 was induced in response to ABA and PEG treatments, implying the potential involvement in ABA-induced drought stress responses. Fluorescence observation suggested that AmSLAC1 was localized in the plasma membrane. BiFC asssays demonstrated an interaction between AmSLAC1 and the typical calcium-dependent protein kinases AmCPK6. Ectopic expression of AmSLAC1 restores a slac1-defective phenotype in Arabidopsis. Furthermore, anion conductance mediated by AmSLAC1 can be activated by AmCPK6 in Xenopus oocytes. Taken together, these results demonstrate that the expression of AmSLAC1 enables the complement of the phenotypes of Arabidopsis slac1 mutants, indicating that AmSLAC1, as an anion channel and regulated by AmCPK6, is functionally conserved for ABA and drought induced stomata closure.
Assuntos
Secas , Fabaceae/metabolismo , Estômatos de Plantas/metabolismo , Estômatos de Plantas/fisiologia , Arabidopsis/genética , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Fabaceae/genética , Fabaceae/fisiologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estômatos de Plantas/genética , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Estresse Fisiológico/genética , Estresse Fisiológico/fisiologiaRESUMO
Clinical treatment of large segmental bone defect has always been a major challenge for the medical community,which is due to the complex and diverse pathogenic factors of large segmental bone defect.In recent years,the clinical treatment of large segmental bone defect has made great progress,and the main treatment options include vascularized or non-vascularized autologous bone grafts,allograft bone transplantation,masquelet technology,llizarov technology and bone tissue engineering.Therefore,understanding the advantages and disadvantages of various treatment options is very important for the clinical treatment of large bone defects in long bones,laying the foundation for clinical treatment.
