[Investigating genotype of HCV distribution among residents in a "blood donation" village in Hebei Province].

OBJECTIVE: To investigate genotype of HCV infection among residents in a rural village, Hebei Province. METHODS: Blood samples of the 520 residents were collected, and C/E1 of HCV genome of the 483 samples were obtained by RT-PCR amplifying, and the gene sequences were analyzed and the polygenetic tree were drawn by the software Mega 4.0. RESULTS: In 483 subjects, HCV-RNA positive objectives are 70, positive prevalence is 14.5%, genotyping for all the samples successfully, including genotypes 1b and 2a, which are 36 (51.4%), 34 (48.6%) respectively. CONCLUSION: HCV RNA positive rate is 14.5%, which is higher than general people. Subtypes 1b and 2a seemed to be the dominant genotypes of HCV in Zhao county area of Hebei Province.

[The analysis of "alpha" dominant mutation of hepatitis B virus in community-based Zhengding area, Hebei province].

OBJECTIVE: To determine the "alpha"dominant mutation of hepatitis B virus (HBV) in community-based Zhengding. Analysis the role of the newborn hepatitis B vaccination on the mutation. METHODS: Based on the national surveillance of hepatitis B, 11,478 people's sera were collected and tested by SPRIA with kits. Collect people's sera with positive HBsAg and amplify the S gene. Sequencing and clastwaling them with the standard sequences. RESULTS: Overall, HBV DNA was successfully amplified and sequenced in 434 of 443 samples. 6.7% samples mutated in HBV "alpha" dominant region. The difference between the mutation ratio of the two loops of HBV "alpha" dominant between the people born before and after the year 1986 has no significance. CONCLUSION: There were HBV "alpha" dominant mutant virus in the local area with a low infection rate in the population born after the year 1986. It could not explain the newborn hepatitis B vaccination can induce the prevalence of the "alpha" dominant mutate HBV.

[Study on factors influencing acetabular development after closed reduction in developmental dislocation of the hip].

OBJECTIVE: To explore factors influencing acetabular development after closed reduction in developmental dislocation of the hip (DDH), to provide theoretical basis for improving the therapeutic effects of closed reduction treatment of DDH. METHODS: A total of 100 children with single side DDH who were treated by closed reduction from January 2002 to December 2005 were followed up, and the AI and AI (D/W) of dislocation hip were measured at the 12th month after reduction. Ten factors such as gender, side, age, AI and AI (D/W) of dislocation hip before reduction, degree of dislocation, the rate of width of femoral head, femoral neck anteversion of dislocation hip before reduction, h/b rate,cut off adductor and skeletal traction et al were taken as independent variable, AI and AI (D/W) at the 12th month after reduction were taken as dependent variable,then multiple linear stepwise regression analysis was used to screen the major influencing factors. RESULTS: Age, gender, degree of dislocation, h/b rate, the rate of width of femoral head and femoral neck anteversion exerted obviously influence on AI and AI (D/W),and then age,degree of dislocation and FNA had positive correlation with AI and negative correlation with AI (D/W); the rate of width of femoral head and h/b rate had negative correlation with AI and positive correlation with AI(D/W). The development of acetabular of female patients was faster than that of male patients. CONCLUSION: Age, gender,degree of dislocation, concentric reduction or not, the development degree of femoral head before reduction and proximal femoral shape are the major factors influencing acetabular development.