RESUMO
Lung adenocarcinoma (ADC) is one of the major histological types of lung cancer. Genetic polymorphism in DNA repair genes and lung ADC susceptibility is well documented. In this case-control study, the association between the polymorphic sites of DNA repair genes XPD-751, XRCC1-399, and OGG1-326, and lung ADC susceptibility in ethnic Han Chinese population has been investigated. Genomic DNA was isolated from the peripheral blood of 201 healthy controls and 82 lung ADC patients from the people of Hunan Province, China. Polymorphisms of the investigated genes were analyzed by using polymerase chain reaction-restriction fragment length polymorphism. There was no significant difference between the samples from lung ADC patients and healthy controls about the genotype frequencies of XPD-751, XRCC1-399, and OGG1-326 sites. However, multifactor dimensionality reduction analysis showed that the genetic polymorphisms of the three-loci models of DNA repair genes (XPD-751/XRCC1-399/OGG1-326) are associated with lung ADC. Thus, this study reveals that a three-order interaction among the polymorphic sites of XPD-751, XRCC1-399, and OGG1-326 is associated with lung ADC risk in the studied population, although polymorphism in individual gene was not associated.
Assuntos
Adenocarcinoma/genética , DNA Glicosilases/genética , Proteínas de Ligação a DNA/genética , Neoplasias Pulmonares/genética , Proteína Grupo D do Xeroderma Pigmentoso/genética , Adenocarcinoma de Pulmão , Adulto , Idoso , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição , Fatores de Risco , Análise de Sequência de DNA , Proteína 1 Complementadora Cruzada de Reparo de Raio-XRESUMO
Lung cancer is a common cause of cancer-related death. The link between risk of lung cancer susceptibility and genetic polymorphisms in metabolic enzymes is well documented. In this study, the relationships between lung cancer susceptibility and polymorphisms in the phase I metabolic enzyme genes CYP1A1, CYP2D6, and CYP2A6 were investigated. Genomic DNA was isolated from the peripheral blood of 201 healthy controls and 168 lung carcinoma patients from the Han ethnic group of Hunan Province in Central South China. Polymorphisms of the investigated genes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and two-step allelic-specific PCR assays. No significant differences were found between the frequencies in cases and controls for the genotypes wild-type (WW), heterozygous mutant, or homozygous mutant; for CYP1A1 or CYP2D6; or for the genotypes WW, heterozygous deletion, or null genotype for CYP2A6. The three-locus model (CYP2A6/CYP1A1/CYP2D6) had a maximum test sample accuracy that was significant (P < 0.001) with a cross-validation consistency of 10. These results indicated that the three-order interaction of CYP2A6, CYP1A1, and CYP2D6 polymorphisms might increase genetic susceptibility to lung cancer. We report the involvement of a three-order interaction between CYP1A1, CYP2A6, and CYP2D6 polymorphisms in lung cancer risk in people in Central South China, although no relationship between lung cancer risk and individual gene polymorphisms was found.
Assuntos
Hidrocarboneto de Aril Hidroxilases/genética , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP2D6/genética , Neoplasias Pulmonares/etnologia , Neoplasias Pulmonares/genética , Polimorfismo Genético/genética , Adulto , Biomarcadores Tumorais/genética , Estudos de Casos e Controles , China/etnologia , Citocromo P-450 CYP2A6 , Feminino , Humanos , Neoplasias Pulmonares/diagnóstico , Masculino , Pessoa de Meia-IdadeRESUMO
OBJECTIVE: To estimate the psychological health status of attacking stage patients with cerebral arterial sclerosis(CAS). METHODS: Sixty in-patients were surveyed by filling up the questionnaire (SCL-90) and compared with the customary model in China. RESULTS: The symptomatic factor scores of somatization and depression of the patients were obviously higher than those of the customary model (P < 0.001). Their symptomatic factor scores of anxiety and obsessive-compulsive were higher than those of the customary model (P < 0.05). The symptomatic factor score of phobic anxiety was higher than that of the customary model (P < 0.01). CONCLUSION: Somatization, obsessive-compulsive, depression, anxiety and phobic anxiety are common psychological problems. Corresponding nursing countermeasures are proposed in our study.
