RESUMO
This article corrects: Synergistic effect of PCPE1 and sFRP2 on the processing of procollagens via BMP1, Volume 593, Issue 1, 119-127. Article first published 09 November 2018. https://doi.org/10.1002/1873-3468.13291 Dr Daniel S. Greenspan was inadvertently omitted from the list of authors in the original publication. The correct list of authors is as shown above. Dr Daniel S. Greenspan affiliation and contact details are as follows: Department of Cell and Regenerative Biology, University of Wisconsin, Room 4503, WIMRII, 1111 Highland Ave, Madison, WI 53705, dsgreens@wisc.edu.
RESUMO
Procollagen processing is essential for organ development and tissue functions. Both procollagen C-proteinase enhancer 1 (PCPE1) and secreted frizzled-related protein 2 (sFRP2) play vital roles in collagen formation via regulating the procollagen C-proteinase activity of bone morphogenetic protein 1 (BMP1). However, whether the two proteins exert a synergistic effect on BMP1 activity remains unclear. Here, simultaneous knockdown of sFRP2 and PCPE1 led to less collagen formation in mouse embryonic fibroblasts and dorsalized phenotypes in zebrafish embryos. Further studies revealed a direct interaction between the Frizzled domain of sFRP2 and the complement/Uegf/BMP-1 domain of PCPE1, which enhances the cleavage activity of BMP1 on procollagen. These results suggest that double silencing of sFRP2 and PCPE1 may provide a strategy for treating fibrosis diseases caused by collagen deposition.
Assuntos
Proteína Morfogenética Óssea 1/metabolismo , Glicoproteínas/metabolismo , Proteínas de Membrana/metabolismo , Peixe-Zebra/crescimento & desenvolvimento , Animais , Sítios de Ligação , Padronização Corporal , Células Cultivadas , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Fibroblastos/citologia , Fibroblastos/metabolismo , Técnicas de Silenciamento de Genes , Glicoproteínas/química , Glicoproteínas/genética , Proteínas de Membrana/genética , Camundongos , Peixe-Zebra/embriologia , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/química , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismoRESUMO
Sediments from 14 stations in the Foshan Waterway, a river crossing the industrial district of Guangdong Province, South China, were sampled and subsequently analyzed. The 14 stations were selected for the pollution discharging features of the river, such as the hydrology, the distribution of pollution sources, and the locations of wastewater outlets. The ecological risks were assessed, and the pollution sources were identified to provide valuable information for environmental impact assessment and pollution control. The spatial variability was high and the range were (in milligrams per kilogram dry weight): Pb, 46.0~382.8; Cu, 33.7~ 482.3; Zn, 62.2~1,568.7; Ni, 28.5~130.7; Cr, 34.7~1,656.1; Cd, 0.50~8.53; Hg, 0.02~8.27; and As, 5.77~66.09. The evaluation results of enrichment factor and potential ecological risk index indicate that the metal pollution in the surface and bottom sediments were severely polluted and could pose serious threat to the ecosystem in most stations. Although the hazard levels of the trace element differed among the stations, Hg was the most serious pollutant in all stations. The results of principal component analysis (PCA) show that the discharge of industrial wastewater is the most important polluting factor whereas domestic sewage, which contains a large amount of organic substances, accelerates metal deposition. And potential pollution sources were identified by the way of integrating the analysis results of PCA and data gained from the local government. Therefore, the conclusion is drawn that Foshan Waterway is seriously polluted with trace elements, both in the surface sediment (0 to 20 cm) and the bottom sediments (21 to 50 cm) are contaminated.
