RESUMO
OBJECTIVE: To study the optimal extraction and clathration technology of Rhizoma Atractylodis Macrocephalae oil in Heweilichang Pill. METHODS: Orthogonal test was employed for selecting the optimum of extraction technology of Rhizoma Atractylodis Macrocephalae oil and the oil extraction rate were used as a index. The optimum including technology was chosen by determining the oil-bearing rate and extract ratio of inclusion compound. The inclusion compound were identified with thin chromatogram. RESULTS: The extraction technology was 10 volumes of water, extracted 6h with thick granula of Rhizoma Atractylodis Macrocephalae. The optimum preparation conditions for clathrate were established as beta-CD: oil was 6: 1, 3. 5 times of water, triturated for 75 minutes. CONCLUSION: The process is feasible and the method can be used for production.
Assuntos
Medicamentos de Ervas Chinesas/química , Óleos Voláteis/química , Tecnologia Farmacêutica/métodos , beta-Ciclodextrinas/química , Análise de Variância , Cromatografia em Camada Fina , Combinação de Medicamentos , Estabilidade de Medicamentos , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/isolamento & purificação , Óleos Voláteis/análise , Óleos Voláteis/isolamento & purificação , Plantas Medicinais/química , Fatores de TempoRESUMO
OBJECTIVE: To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears. METHODS: Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotype in the Plasmodium vivax merozoite surface protein-1 (PvMSP-1). RESULTS: Target DNA bands appeared in all samples of unstained thick blood smears, while no DNA bands were visible in the fixed and stained thin smears. Both methods identified PvMSP-1 alleles from smears with parasitemia of > or = 0.01%. CONCLUSION: It is feasible to identify PvMSP-1 alleles from Giemsa-stained blood smear.
