Pancreaticoduodenectomy assisted by 3-D visualization reconstruction and portal vein arterialization: A case report (a CARE-compliant article).

BACKGROUND: Three-dimensional visualization reconstruction, the 3-D visualization model reconstructed by software using 2-D CT images, has been widely applied in medicine; but it has rarely been applied in pancreaticoduodenectomy. Although the hepatic artery is very important for the liver, it has to be removed when tumor invades it. Therefore, portal vein arterialization has been used in clinic as a remedial measure, but there still is professional debate on portal vein arterialization. METHODS: Here, we report 1 case that was diagnosed with poorly differentiated adenocarcinoma of the duodenum. The tumor had large size and invaded surrounding organs and vessels. RESULTS: Preliminary diagnoses were poorly differentiated adenocarcinoma of the duodenum and viral hepatitis B. Pancreaticoduodenectomy assisted by 3-D visualization reconstruction and portal vein arterialization were performed in this case. The tumor was removed. Liver function returned to normal limits 1 week after operation. Digital subtraction arteriography showed compensatory artery branches within the liver 1 month after operation. CONCLUSION: 3-D visualization reconstruction can provide a reliable assistance for the accurate assessment and surgical design before pancreatoduodenectomy, and it is certainly worth adopting portal vein arterialization when retention of hepatic artery is impossible or conventional arterial anastomosis is required during pancreatoduodenectomy.

High-level production of a cold-active B-mannanase from Bacillus subtilis BS5 and its molecular cloning and expression.

Mannanases can be useful in the food, feed, pulp and paper industries. In this research a Bacillus subtilis strain (named Bs5) which produced high-level beta-mannanase was isolated. Maximum level of beta-mannanase (1231.41 U/ml) was reached when Bacillus subtilis Bs5 was grown on konjac powder as the carbon source for nine hours at 32 degrees C. The beta-mannanase was a typical cold-active enzyme and its optimal temperature of 35 degrees C was the lowest among those of the known mannanases from bacteria. In addition, the optimal pH was 5.0 and much wide pH range from 3.0-8.0 was also observed in the beta-mannanase. These properties make the beta-mannanase more attractive for biotechnological applications. The DNA sequence coding the beta-mannanase was cloned and the open reading frame consisted of 1089 bp encoding 362 amino acids. A phylogenetic tree of the beta-mannanase based on the similarity of amino acid sequences revealed that the beta-mannanase formed a cluster with the beta-mannanases of Bacillus subtilis, which was separated from the mannanases of fungi and other bacteria. The beta-mannanase gene could be expressed in Escherichia coli and the recombinant beta-mannanase was characterized by Western blot. This study provided a new source of carbohydrate hydrolysis enzyme with novel characteristics from Bacillus subtilis.

[Effect of various factors on ozone inactivating Giardia in water].

In order to study the effect of O3 inactivating Giardia in water, different factors (CT value, pH, temperature, turbidity, organic content and inorganic ions) which might influence the inactivation were investigated by using fluorescence staining method. The results indicated that the whole process of O3 inactivating Giardia could be divided into two periods, the inactivated rate in log phase was significantly faster than it in the slow phase [k1 = (5.64 +/- 0.023) x 10(-1) mg x min, k2 = (2.72 +/- 0.002) x 10(-2) mg x min, k1 >> k2]. When the turbidity was 0.1 to 20. 0NTU, temperature was 5 to 35 degrees C, pH was 6.0 to 9.0, HA content was 0.5 to 10.0 mg/L, the turbidity was lower, the higher inactivating ratio could be received. With the increasing of temperature, the inactivating effect was decreased. The ability of O3 inactivating Giardia was stronger under acidic condition than it was in alkali circumstance. When the reaction system contained higher concentration of organics, the competition reaction might take place between Giardia and organics with O3, which might reduce inactivation ratio. The sequence of affecting disinfectant ability of O3 was NO3- > None > SO4(2-) > HCO3-, while inorganic cations (Ca2+, Mg2+ and Cu2+) promoted the inactive reaction to a certain extent. If the CT value of O3 was more than 15.0 min x mg/L, the ratio of inactivation could exceed 99.0% during disinfecting drinking water.

[Synthesis and photoluminescence of Zn(1-x) Mo(1-y)Si(y)O4 : Eu+ phosphor].

The phosphors Zn(1-x) Mo(1-y)Si(y)O4 : Eu(x)3+ (0.05 < or = x < or = 0.30, 0 < or = y < or = 0.09) were prepared by solid state reaction technique at 800 degrees C. The powder X-ray diffraction patterns of the samples show that the phosphors are of single phase and the doping Eu ion and Si ion have little influence on the host structure. The effects of flux and calcination temperature on the luminescent properties of the phosphors were investigated. The results showed that flux content has effects on the luminescent properties, and the optimized flux content and the best calcination temperature is 4% and 800 degrees C, respectively. The presence of the Na+ ion strengthens the photoluminescence intensity of the phosphors. The addition of Na+ ions balanced the charge in samples, enhanced the luminescence intensity of samples, and the luminescence intensity reached the maximum when the doping concentration of Na2 CO3 was 4 Wt%. The luminescent properties of Zn0.80 Mo(1-y)Si(y)O4 : Eu(0.20)3+ were studied by the excitation and emission spectra, and the influence of Eu3+ and Si4+ concentrations on the luminescent property was discussed. As the calcination temperature rises from 700 to 800 degrees C, the emission intensity increases due to the improvement of crystallinity. The excitation spectra consist of a broad band and a series of narrow lines, and the narrow lines are attributed to the intrinsic transition from 7FJ (J = 1-4) to 5DJ (J = 0, 1) of Eu3+. It was found that the PL emission intensity was enhanced with the increase in the Eu3+ doping ratio and reached a maximum value at x = 0.20. The result indicated that Zn(1-x)Mo(1-y)Si(y)O4 : Eu(x)3+ phosphors can be excited effectively at 393 and 464 nm light. The presence of the Si4+ ion strengthens the photoluminescence intensity of the phosphors and the strong red emission lines at 616 nm correspond to the forced electric dipole 5D0 --> F2 transitions on Eu3+. Compared with Y2O2S : 0.05 Eu3+, the obtained Zn0.80Mo0.97Si0.03O4 : Eu(0.20)3+ phosphor shows an enhanced red emission under 393 nm excitation and the emission intensity of Y2O2S : 0.05Eu3+ is only 50% of that of Zn0.80Mo0.97Si0.03O4 : Eu(0.20)3+. The optical properties suggest that Zn0.80Mo0.97Si0.03O4 : Eu(0.20)3+ is an efficient red emitting phosphor for light emitting diode (LED) applications.

[Analysis of signal peptides of the secreted proteins in Ralstonia solanacearum GMI1000].

The 3,440 open reading frames (ORFs) of Ralstonia solanacearum GMI1000 were used for predicting signal peptides by comprehensive analyses with SignalP 3.0, TMHMM 2.0, TargetP 1.01, LipoP 1.0 and PSORTb, and screening based on L value. Total 186 signal peptides with conserved amino acid residues are found and among them, 134 are secretary types, 22 are RR-motif types and 30 are lipoprotein signal peptides. The length distribution of signal peptide and its domains were analyzed systemically. No type pilin-like signal peptides and bacteriocins and pheronones signal peptides are found in the database of deduced proteins encoded by the genome of R. solanacearum GMI1000.

Treatment of phenol wastewater by microwave-induced ClO2-CuOx/Al203 catalytic oxidation process.

The catalyst of CuOx/Al2O3 was prepared by the dipping-sedimentation method using gamma-Al2O3 as a supporter. CuO and Cu2O were loaded on the surface of Al2O3, characterized by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). In the presence of CuOx/Al2O3, the microwave-induced chlorine dioxide (ClO2) catalytic oxidation process was conducted for the treatment of synthetic wastewater containing 100 mg/L phenol. The factors influencing phenol removal were investigated and the results showed that microwave-induced ClO2-CuOx/Al2O3 process could effectively degrade contaminants in a short reaction time with a low oxidant dosage, extensive pH range. Under a given condition (ClO2 concentration 80 mg/L, microwave power 50 W, contact time 5 min, catalyst dosage 50 g/L, pH 9), phenol removal percentage approached 92.24%, corresponding to 79.13% of COD(Cr) removal. The removal of phenol by microwave-induced ClO2-CuOx/Al2O3 catalytic oxidation process was a complicated non-homogeneous solid/water reaction, which fitted pseudo-first-order by kinetics. Compared with traditional C1O2 oxidation, ClO2 catalytic oxidation and microwave-induced ClO2 oxidation, microwave-induced ClO2 catalytic oxidation system could significantly enhance the degradation efficiency. It provides an effective technology for the removal of phenol wastewater.

Determination of trace copper by solid substrate-room temperature phosphorescence quenching method based on activating effect of alpha,alpha'-dipyridyl on Vitamin C reducing beryllon.

A new solid substrate-room temperature phosphorescence quenching method for the determination of trace copper has been established. It is based on the fact that beryllon (R) can emit strong and stable solid substrate-room temperature phosphorescence on the filter paper, and Vitamin C (Vc) reduces R to non-phosphorescent compound that leads to solid substrate-room temperature phosphorescence (SS-RTP) quenching of R, and alpha,alpha'-dipyridyl can activate copper catalyzing Vitamin C reducing R. The DeltaI(p) of the system with alpha,alpha'-dipyridyl is 3.3 times higher than that without alpha,alpha'-dipyridyl, which shows the reaction of alpha,alpha'-dipyridyl activating copper catalyzing Vitamin C reducing R. The reducing value of phosphorescence intensity (DeltaI(p)) is directly proportional to the content of Cu(II) in the range of 0.040-4.0 fg spot(-1) (corresponding concentration: 0.10-10.0 pg ml(-1), sample volume: 0.40 microlspot(-1)). The regression equation of working curve can be expressed as DeltaI(p)=69.99+41.00 m Cu(2+) (fg spot(-1)) (r=0.9980, n=6), and the detection limit is 0.0088 fg spot(-1)(corresponding concentration: 2.2 x 10(-14) g ml(-1)). This sensitive and accurate method with good repeatability and high selectivity has been applied to the determination of trace copper in real samples with satisfactory results. The reaction mechanism for the determination of trace copper by solid substrate-room temperature phosphorescence quenching method based on the activating effect of alpha,alpha'-dipyridyl on Vitamin C reducing beryllon is also discussed.

[Bioflocculant producing capability by two strains of Bacillus sp. in diversified carbon sources].

Two strains of Bacillus sp. F2 and F6 could produce bioflocculants with high flocculating effects by pure culture and mixed culture. Many kinds of carbon sources could be utilized. Organic compound which molecular weight under 200 such as saccharide, alcoholic aldehyde, ester and organic acid were favorable for producing bioflocculant. And some low-cost biomass scrap could be used as good carbon sources for industrialized production of bioflocculants, such as waste beet molasses, cellulose's fermentation residue and sewage of hydrogen producing reactor.

[The research progress on molecular genetics of pathogenicity of rice blast fungus.].

Rice blast disease, caused by heterothallic ascomycete Magnaporthe grisea, is one of the most serious fungal diseases of rice throughout the world. The disease attacks rice plants throughout the season and causes severe yield losses. The pathogenesis of M. grisea is due to a complex process that spans the entire life cycle of the pathogen. The process including germination of conidia, formation of appressoria, differentiation of penetration pegs and proliferation of infectious hyphae is controlled by many genes. The interaction between M. grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence (Avr) gene and the host resistance (R) gene. The studies on molecular biology and genetic mechanism of pathogenicity of M. grisea has occupied pathologists and mycologists for several decades. This paper reviews the research progress related to molecular genetics of pathogenicity of the fungus and its genetic diversity and variation, and summarize research methods of the functional genes.

[Kinetics and mechanism of aniline oxidation with chlorine dioxide in water].

Reaction kinetics of aniline oxidation with chlorine dioxide in water were investigated systematically by detecting concentrations of aniline with HPLC at regular intervals. Results showed that the reaction was first-order with respect to both chlorine dioxide and aniline, and the entire reaction was of second-order. Under condition of pH 6.86 and water temperature (Tw) 287 K, the second-order reaction rate constant k was 0.11 L x (mol x s)(-1). Reaction activation energy was 73.11 kJ x mol(-1), revealing that the reaction could take place under usual water treatment conditions. The reaction rate constants in acidic and alkali media were greater than that in neutral medium. Effect of chlorite ion on the reaction rate was insignificant. P-aminophenol and azobenzene were detected by GC MS as intermediates. Oxidation pathways were proposed and discussed elementarily.

Kinetics of aniline oxidation with chlorine dioxide.

For the first time, kinetics of aniline oxidation with chlorine dioxide (ClO2) were investigated systematically by detecting concentration of aniline with HPLC at regular intervals. Results showed that the reaction was first-order both in ClO2 and in aniline, and the oxidation reaction could be described as second-order reaction. Stoichiometric factor eta was experimentally determined to be 2.44. The second-order-reaction rate constant k was 0.11 L/(mol x s) under condition of pH 6.86 and water temperature (Tw) 287K. Reaction activation energy was 72.31 kJ/mol, indicating that the reaction could take place under usual water treatment conditions. The reaction rate constants in acidic and alkali media were greater than that in neutral medium. Chlorite ion could slightly increase reaction rate in acidic medium. p-aminophenol and azobenzene were detected by GC-MS as intermediates.