Genome-wide identification of bHLH transcription factors and expression analysis under drought stress in Pseudoroegneria libanotica at germination.

The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in Pseudoroegneria species has not been studied yet. Pseudoroegneria (Nevski) Á. Löve is a perennial genus of the Triticeae. Pseudoroegneria species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in Pseudoroegneria libanotica, which could be classified into 15 groups. Collinearity analysis indicates that 122 PlbHLH genes share homology with wbHLH genes in wheat, and it has lower homology with AtbHLH genes in Arabidopsis. Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated PlbHLH genes were screened. Among them, PlbHLH6, PlbHLH55 and PlbHLH64 as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of PlbHLHs in Pse. libanotica, and discovered new drought-tolerance candidate genes to enhance the genetic background of Triticeae crops. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01433-w.

Biosystematics studies on Elymus breviaristatus and Elymus sinosubmuticus (Poaceae: Triticeae).

BACKGROUND: Elymus breviaristatus and Elymus sinosubmuticus are perennial herbs, not only morphologically similar but also sympatric distribution. The genome composition of E. sinosubmuticus has not been reported, and the relationship between E. sinosubmuticus and E. breviaristatus is still controversial. We performed artificial hybridization, genomic in situ hybridization, and phylogenetic analyses to clarify whether the two taxa were the same species. RESULTS: The high frequency bivalent (with an average of 20.62 bivalents per cell) at metaphase I of pollen mother cells of the artificial hybrids of E. breviaristatus (StYH) × E. sinosubmuticus was observed. It illustrated that E. sinosubmuticus was closely related to E. breviaristatus. Based on genomic in situ hybridization results, we confirmed that E. sinosubmuticus was an allohexaploid, and the genomic constitution was StYH. Phylogenetic analysis results also supported that this species contained St, Y, and H genomes. In their F1 hybrids, pollen activity was 53.90%, and the seed setting rate was 22.46%. Those indicated that the relationship between E. sinosubmuticus and E. breviaristatus is intersubspecific rather than interspecific, and it is reasonable to treated E. sinosubmuticus as the subspecies of E. breviaristatus. CONCLUSIONS: In all, the genomic constitutions of E. sinosubmuticus and E. breviaristatus were StYH, and they are species in the genus Campeiostachys. Because E. breviaristatus was treated as Campeistachys breviaristata, Elymus sinosubmuticus should be renamed Campeiostachys breviaristata (Keng) Y. H. Zhou, H. Q. Zhang et C. R. Yang subsp. sinosubmuticus (S. L. Chen) Y. H. Zhou, H. Q. Zhang et L. Tan.

Heat-inactivated Escherichia coli promotes hematopoietic regeneration after irradiation with IL-1ß.

BACKGROUND AIMS: Hematopoietic stem and progenitor cells (HSPCs) are known to produce short-lived mature blood cells via proliferation and differentiation in a process that depends partially on regulatory cytokines from the bone marrow (BM) microenvironment. Delayed BM recovery after tremendous damage to the hematopoietic system can lead to neutropenia, anemia, thrombopenia and even death. However, efficiently promote BM recovery is still a big problem to be solved. Here, the authors aim to use heat-inactivated Escherichia coli (HIEC) to enhance BM recovery and further to find out the potential mechanism. METHODS: X-rad was used to establish HIEC/IL-1ß-induced radioprotection model. Single-cell RNA sequencing, RT-PCR, and western blotting were performed to detect the expression of IL-1R1 on HSPCs. Flow cytometry and automated hematology analyzer were used to analyze the percentage and absolute number of different populations of hematopoietic cells. The effects of IL-1ß on HSPCs were studied using in vivo and in vitro experiments. RESULTS: HIEC/IL-1ß pre-treatment can significantly increase the survival rate of lethally irradiated mice, and these mice showed better hematopoietic regeneration compared with control group. IL-1R was expressed on HSPCs, and IL-1ß could directly function on HSPCs to promote the proliferation and differentiation of HSPCs, and inhibit the apoptosis of HSPCs. CONCLUSIONS: HIEC pre-treatment can rescue lethally irradiated mice by promoting hematopoietic recovery via IL-1ß/IL-1R1 signaling, which can promote the proliferation of HSPCs by enhancing the cell cycle and attenuating the apoptosis of HSPCs.

Subsidence prediction of overburden strata and ground surface in shallow coal seam mining.

Shallow coal seam with thick soil layer is widely reserved in the Jurassic Coalfield, Western China, mining-induced subsidence represents complex characteristics. Combining with physical simulation, theoretical analysis and in-situ observation, the overburden strata structure in dip direction were revealed, and the subsidence prediction models were established, based on this, the subsidence equations of overburden strata and ground surface were proposed. The results show that after shallow coal seam mining, based on the subsidence and movement characteristics, the overburden strata structure can be divided into three zones, which are "boundary pillar F-shape zone" (BPZ), "trapezoid goaf zone" (TGZ) and "coal pillar inverted trapezoidal zone" (CPZ). The subsidence of overburden strata depends on the key stratum, while the subsidence of soil layer depends on the bedrock subsidence basin, which is between the bedrock and thick soil layer. The bedrock subsidence is mainly related to mining height and bulking coefficient in TGZ, while it is mainly affected by mining height and distribution load on the key stratum in BPZ and CPZ. According to physical simulation and theoretical model, the maximum surface subsidence of No.1-2 seam mining in Ningtiaota coal mine are 1.1 m and 1.07 m respectively, which is basically consistence with the result of in-situ observation (1.2 m).

Roof structure of shallow coal seam group mining in Western China.

In order to realize roof control of shallow coal seam group mining in Western China, combining with engineering statistics, physical simulation and theoretical analysis, the roof weighting characteristics during lower coal seam mining were revealed, and the classification of shallow coal seam group was proposed. Based on this, mechanical models of roof structure were set up, and the calculation method of support resistance was determined. The results show that the roof weighting is closely related to the interburden thickness and the mining height of lower coal seam, considering the ratio of interburden thickness to the mining height, as well as the key stratum structure, the classification of shallow coal seam group was put forward. The first type is shallow coal seam group with no key stratum (SCSG-No), its roof pressure is mainly affected by caving roof of upper coal seam, and the interburden roof forms slanting pillar-beam structure. The second type is shallow coal seam group with single key stratum (SCSG-S), interburden roof represents step voussoir beam structure. The third type is shallow coal seam group with double key strata (SCSG-D), interburden roof can form double key strata structure, the lower key stratum forms slanting step voussoir beam structure, while the upper key stratum forms voussoir beam structure, besides, longwall face represents large-small periodic weighting. Through establishing the roof structure models, the calculation formulas of support resistance were determined, it can provide basis for roof control and promote safe mining in Western China.