High precision vibration sectioning for 3D imaging of the whole central nervous system.

BACKGROUND: Imaging and reconstruction of the morphology of neurons within the entire central nervous system (CNS) is important for deciphering the neural circuitry and related brain functions. With combination of tissue clearing and light sheet microscopy, previous studies have imaged the mouse CNS at cellular resolution, while remaining single axons unresolvable due to the tradeoff between sample size and imaging resolution. This could be improved by sectioning the sample into thick slices and imaged with high resolution light sheet microscopy as described in our previous study. However, the achievable quality for 3D imaging of serial thick slices is often hindered by surface undulation and other artifacts introduced by sectioning and handling limitations. NEW METHODS: In order to improve the imaging quality for mouse CNS, we develop a high-performance vibratome system for sample sectioning and handling automation. The sectioning mechanism of the system was modeled theoretically and verified experimentally. The effects of process parameters and sample properties on sectioning accuracy were studied to optimize the sectioning outcome. The resultant imaging outcome was demonstrated on mouse samples. RESULTS: Our theoretical model of vibratome effectively depicts the relationship between the sample surface undulation errors and the sectioning parameters. With the guidance of the theoretical model, the vibratome is able to achieve a local surface undulation error of ±0.5 µm and a surface arithmetic mean deviation (Sa) of 220 nm for 300-µm-thick tissue slices. Imaging results of mouse CNS show the continuous sectioning capability of the vibratome. COMPARISON WITH EXISTING METHOD: Our automatic sectioning and handling system is able to process serial thick slices for 3D imaging of the whole CNS at a single-axon resolution, superior to the commercially available vibratome devices. CONCLUSION: Our automatic sectioning and handling system can be optimized to prepare thick sample slices with minimal surface undulation and manual manipulation in support of 3D brain mapping with high-throughput and high-accuracy.

[Level of plasma lysophosphatidic acid in patients with cerebral ischemic attack, a preliminary study].

OBJECTIVE: To study the level of plasma lysophosphatidic acid (LPA) in patients with TIA, cerebral thrombosis or possible ischemic attack. METHODS: Phospholipid was extracted from plasma of patients with TIA, cerebral thrombosis or possible ischemic attack by organic solvent. Lysophosphatidic acid was isolated further. The quantity of LPA was determined by assaying its phosphorous component. RESULTS: LPA level of patients with TIA, cerebral thrombosis or possible ischemic attack were 2.14 +/- 1.02, 1.50 +/- 0.85, and 1.73 +/- 1.14 micromol/L blood plasma respectively, all significantly higher than that in control group (1.00 +/- 0.70 micromol/L, P < 0.05). The plasma LPA level decreased after anticoagulant treatment. CONCLUSION: As a molecular marker released by activated platelets in vivo, plasma PLA may be valuable in diagnosis of cerebral ischemic attack.