Love Hug-Functional Validation of Nuptial Pad-Secreted Pheromone in Anurans.

Chemical communication is an important mode of communication in the courtship and breeding processes of amphibians. In caudates, multiple components of sexual pheromones have been identified and functionally verified. One of these pheromone systems is plethodontid modulating factor (PMF). In anurans, the pheromone called amplexin was found in nuptial pads of ranids and was considered a member of the PMF system, yet its bio-function has not been tested. In this study, we obtained 18 amplexin transcript sequences from nuptial pads of Nidirana pleuraden (Amphibia, Ranidae) by transcriptome sequencing and found that the proteins translated by these transcripts are diversified, hydrophilic, and relatively stable. We also acquired a N. pleuraden amplexin isoform with the highest expression level in the transcriptome analysis through the prokaryotic expression system. Using two different animal behavioral experimental settings, we have tested the bio-function of the recombinant PMF protein (rPMF) in N. pleuraden's reproduction and found that the rPMF does not attract females but shortens the duration of amplexus significantly. This is the first study to verify the function of the PMF pheromone in Anura, indicating the pervasiveness of chemical communication during breeding in amphibians.

Efficacy of simultaneous hexavalent chromium biosorption and nitrogen removal by the aerobic denitrifying bacterium Pseudomonas stutzeri YC-34 from chromium-rich wastewater.

The impact of high concentrations of heavy metals and the loss of functional microorganisms usually affect the nitrogen removal process in wastewater treatment systems. In the study, a unique auto-aggregating aerobic denitrifier (Pseudomonas stutzeri strain YC-34) was isolated with potential applications for Cr(VI) biosorption and reduction. The nitrogen removal efficiency and denitrification pathway of the strain were determined by measuring the concentration changes of inorganic nitrogen during the culture of the strain and amplifying key denitrification functional genes. The changes in auto-aggregation index, hydrophobicity index, and extracellular polymeric substances (EPS) characteristic index were used to evaluate the auto-aggregation capacity of the strain. Further studies on the biosorption ability and mechanism of cadmium in the process of denitrification were carried out. The changes in tolerance and adsorption index of cadmium were measured and the micro-characteristic changes on the cell surface were analyzed. The strain exhibited excellent denitrification ability, achieving 90.58% nitrogen removal efficiency with 54 mg/L nitrate-nitrogen as the initial nitrogen source and no accumulation of ammonia and nitrite-nitrogen. Thirty percentage of the initial nitrate-nitrogen was converted to N2, and only a small amount of N2O was produced. The successful amplification of the denitrification functional genes, norS, norB, norR, and nosZ, further suggested a complete denitrification pathway from nitrate to nitrogen. Furthermore, the strain showed efficient aggregation capacity, with the auto-aggregation and hydrophobicity indices reaching 78.4 and 75.5%, respectively. A large amount of protein-containing EPS was produced. In addition, the strain effectively removed 48.75, 46.67, 44.53, and 39.84% of Cr(VI) with the initial concentrations of 3, 5, 7, and 10 mg/L, respectively, from the nitrogen-containing synthetic wastewater. It also could reduce Cr(VI) to the less toxic Cr(III). FTIR measurements and characteristic peak deconvolution analysis demonstrated that the strain had a robust hydrogen-bonded structure with strong intermolecular forces under the stress of high Cr(VI) concentrations. The current results confirm that the novel denitrifier can simultaneously remove nitrogen and chromium and has potential applications in advanced wastewater treatment for the removal of multiple pollutants from sewage.

Broadband surface wave coupler with low infrared emission and microwave reflection.

Metasurfaces possess excellent capabilities to flexibly manipulate electromagnetic waves in multiple frequency domains, which show great potential application in multispectral stealth. Herein, a broadband surface waves coupler based on the design of thin Pancharatnam-Berry (PB) phase gradient metasurfaces (PGMs) of thickness 0.12λ0 is proposed to reduce infrared emission and microwave reflection simultaneously. Low infrared emission results from the high filling ratio of the indium-tin-oxide (ITO) on the surface, and low microwave reflection results from the conversion from propagating waves to surface waves. Intriguingly, this design is also capable of acting as a simple circular polarized (CP) discriminator because orthogonal CP waves are coupled into surface waves propagating along opposite directions. A proof-of-concept prototype is simulated and measured to validate the effectiveness of our methodology. The results indicate that the broadband surface waves coupler shows low infrared emissivity less than 0.28 from 3 to 14 µm and has microwave reflection reduction larger than 10 dB in 7.3-9.5 GHz. The exceptional performances of the proposed broadband surface waves coupler make us believe that our design offers an alternative strategy for multispectral stealth and multifunctional application.

Simultaneous reduction of microwave reflection and infrared emission enabled by a phase gradient metasurface.

Metasurfaces have shown promising applications in radar-infrared compatible stealth because of its superior electromagnetic wave control capabilities, but, to date, the majority of designs still suffer from the defects of large thickness, limited working bandwidth, relatively high infrared emissivity and so on. Here, an exotic phase gradient metasurface (PGM) is proposed to achieve low microwave reflection and low infrared emission concurrently, which has a small thickness of about 0.10λ0. The microwave reflection reduction larger than 10 dB in 14-20 GHz is attributed to the anomalous reflection for arbitrary LP incident waves, and the infrared emissivity less than 0.28 from 3 to 14 µm is due to the indium-tin-oxide (ITO) with low infrared emissivity and high filling ratio. Also, the designed PGM can also realize beam deflection for orthogonal CP waves because of the meta-atoms' isotropic characteristics. Our methodology is fully verified by numerous simulations and experiments and may open a new avenue for radar-infrared compatible stealth research.

Optically transparent coding metasurface with simultaneously low infrared emissivity and microwave scattering reduction.

In this paper, an optically transparent coding metasurface structure based on indium tin oxide (ITO) thin films with simultaneously low infrared (IR) emissivity and microwave scattering reduction is proposed. To this end, two ITO coding elements which can reflect 0° and 180° phase responses are firstly designed. Based on these two elements, four coding sequences with different scattering patterns are designed. Three of them can realize anomalous reflections and the fourth can realize random diffusion of normal incident electromagnetic (EM) waves. A prototype of the random diffusion coding metasurface was fabricated and measured. The experimental results show that for normal incident EM waves, at least 10dB backward scattering reduction from 3.8GHz to 6.8GHz can be achieved, and the structure is polarization insensitive. The averaged transmittance of visible light through the coding metasurface reaches up to 72.2%. In addition, due to the high occupation ratio of ITO on the outside of the coding metasurface, a low IR emissivity of about 0.275 is obtained. Good consistency between the experiment and simulation results convincingly verifies the coding metasurface. Due to its multispectral compatibility, the proposed coding metasurface may find potential applications in multi-spectral stealth, camouflage, etc.