RESUMO
A full-length Cks1 homologue gene, AmphiCks1, was identified in amphioxus, Branchiostoma belcheri tsingtauense. Sequence characteristics, phylogeny and patterns of expression during embryonic and larval development were established. The protein predicted from AmphiCks1 showed high sequence identity with vertebrate and invertebrate homologues. Protein structural studies and phylogenetic analysis suggested that Cks homologues are evolutionarily conserved. The AmphiCks1 transcript was detected in most early developmental stages by northern blotting and whole-mount in situ hybridization, suggesting a role for the gene in cell division.
Assuntos
Proteínas de Ciclo Celular/genética , Cordados não Vertebrados/genética , Embrião não Mamífero/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteínas de Ciclo Celular/metabolismo , Cordados não Vertebrados/embriologia , Cordados não Vertebrados/crescimento & desenvolvimento , Bases de Dados de Ácidos Nucleicos , Biblioteca Gênica , Larva/genética , Larva/metabolismo , Dados de Sequência Molecular , Filogenia , RNA Mensageiro/metabolismo , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
The expression pattern of profilin homologue in embryogenesis of amphioxus was characterized by whole-mount in situ hybridization combined serial histological sections and Northern blot. The results revealed that the dynamic expression pattern of the profilin homologue just accorded with muscle differentiating in this animal. It was most possibly involved in the development of muscle in amphioxus. The result of Southern blot supported that there was only one copy of profilin homologue in the genome of Qingdao amphioxus. It may be good for explaining the molecular mechanism of muscle differentiation in amphioxus.
