Transient Nonlinear Heat Conduction in Concrete Structures: A Semi-Analytical Approach.

Thermal loading, especially in fire scenarios, challenges the safety and long-term durability of concrete structures. The resulting heat propagation within the structure is governed by the heat conduction equation, which can be difficult to solve analytically because of the nonlinearity related to the thermophysical properties of concrete. A semi-analytical approach for the transient nonlinear heat conduction problem in concrete structures was established in the present work. The nonlinearity related to the temperature-dependent thermal conductivity, mass density, and specific heat capacity of heated concrete was taken into consideration. A Taylor series approximate solution was first established within a small neighborhood, employing the Boltzmann transformation in combination with the mean value theorem. Thereafter, it was extended to the whole domain by utilizing the Bernstein polynomial. The semi-analytical approach was validated by comparing it with the numerical results of two independent Finite Element simulations of nonlinear heat conduction along concrete plates, subjected to either moderate or fierce thermal loading. Absolute values of the relative errors are smaller than 5%. The validated semi-analytical approach was further applied to prediction of the temporal evolution of the temperature field of a scaled model of a subway station, subjected to fire disaster. The nonlinearities, related to the time-dependent surface temperature and the temperature-dependent thermophysical properties of concrete, were taken into consideration. The predictions agree well with the experimental measurements. The established semi-analytical approach exhibits good accuracy and stability, providing insight into the interaction between the thermophysical properties of concrete in the heat conduction process.

Exploring the environmental efficiency of airlines through a parallel RAM approach.

Air pollution in the aviation industry is becoming increasingly severe worldwide, along with rapid economic development. Therefore, it is significant to pay close attention to airlines worldwide. Usually, the airlines contain passenger transportation and freight transportation on the operating move. This paper proposes a parallel range adjusted measure (PRAM) to comprehensively measure and evaluate the environmental efficiency of 18 airlines from 2014 to 2019. Different from existing models, the model can handle shared inputs, shared desirable outputs, and shared undesirable outputs simultaneously. We build a shared resource decomposition procedure to perform a comparative analysis of the highest subsystem efficiency, and the sensitivity analysis proves the validity of the results. The main findings are as follows: 1. The optimal efficiency can be achieved by most of the 18 airlines when sharing resources; 2. Operating costs in the freight system should be increased to achieve optimal efficiency; 3. Asian airlines show higher efficiency than the airlines in Europe.

Exploring the effect of COVID-19 on airline environmental efficiency through an interval epsilon-based measure model.

COVID-19 has dealt an unprecedented blow to the aviation industry since 2020. This paper applies the interval epsilon-based measure (IEBM) model to evaluate the optimal quarterly environmental efficiency of 14 global airlines of passenger and cargo subsystems during 2018-2020. Then, the time series prediction method is applied to forecast the interval data of inputs and outputs from 2021 to 2022. Finally, we can calculate the quarterly efficiency. Thus, the future development trends of airlines can be predicted. The results show that (1) COVID-19 has hit the passenger subsystem harder, while the freight subsystem has become more efficient; (2) the efficiency of the freight subsystem has inevitably declined in the post-epidemic era; and (3) therefore, the airlines will have a "√" shaped recovery curve in the next few years.

Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2.

BACKGROUND: Stromal cell-derived factor 1 (SDF-1) is an important chemokine for stem cell mobilization, and plays a critical role in mobilization of mesenchymal stem cells (MSCs). Bone morphogenetic protein 2 (BMP-2) plays a critical role in osteogenesis of MSCs. However, the use of SDF-1 and BMP-2 in bone tissue engineering is limited by their short half-lives and rapid degradation in vitro and in vivo. METHODS: The chitosan oligosaccharide/heparin nanoparticles (CSO/H NPs) were first prepared via self-assembly. Chitosan-agarose-gelatin (CAG) Scaffolds were then synthesized via gelation technology using cross-linked chitosan, agarose, and gelatin, and were modified by CSO/H NPs. The encapsulation efficiency and release kinetics of SDF-1 and BMP-2 were quantified using an enzyme-linked immunosorbent assay. A CCK-8 assays were used to evaluate biocompatibility of NP-modified scaffolds. The biological activity of the loaded SDF-1 and BMP-2 was evaluated using the transwell migration assay and osteogenic induction assay. An animal MSC recruitment model was used to study the ability of SDF-1 released from NP-modified scaffolds to induce migration of MSCs. RESULTS: In this study, we developed a novel nanoparticle-modified CAG scaffold for the delivery of SDF-1 and BMP-2. CCK-8 assays demonstrated excellent biocompatibility of NP-modified scaffolds. In addition, we investigated the release of SDF-1 and BMP-2 from NP-modified scaffolds, and evaluated the effect of released SDF-1 on MSC migration. The effect of released BMP-2 on MSC osteogenesis was also examined. In vitro cell migration assays showed that SDF-1 released from NP-modified scaffolds retained its migration activity; osteogenesis studies demonstrated that released BMP-2 exhibited a strong ability to induce differentiation towards osteoblasts. Our in vivo recruitment assays showed continuous chemotactic response of MSCs to SDF-1 released from the NP-modified scaffold. CONCLUSION: The simplicity of synthesizing CSO/H NP-modified CAG scaffolds, combined with its high cytokine loading capacity and sustained release effect, renders NP-modified CAG scaffold an attractive candidate for sustained release of SDF-1 and BMP-2 to promote bone repair and regeneration.

Expression and purification of a rapidly degraded protein, TMEM8B-a, in mammalian cell line.

TMEM8B-a protein is the longer, predominant isoform of the TMEM8B gene product, which is a tumor metastasis suppressor in nasopharyngeal carcinoma (NPC) and lung cancer. TMEM8B-a is rapidly degraded via the proteasome pathway mediated by ezrin in many NPC and lung cancer cell lines, but TMEM8B-a is not ubiquitinated. In this study, we report the recombinant production of full-length modified TMEM8B-a in mammalian cells. We used the PiggyBac transposon system to efficiently generate normal and lung cancer cell lines with stable TMEM8B-a protein expression. 293FT cells were the best host cell line to express TMEM8B-a protein. Then, we treated the stable 293FT cell lines with various small-molecule inhibitors and demonstrated that treatment with MG-132 and bortezomib, which target the proteasome and disrupt its function, could prevent TMEM8B-a degradation and induce protein expression in 293FT cells. Finally, we utilized the combination of Twin-Strep-tag and Strep-Tactin XT resin to successfully purify the TMEM8B-a protein. The final yield was estimated to be approximately 10-20 µg of the purified TMEM8B-a per 3.0 × 108 293FT cells.