RESUMO
PURPOSE: Study of the molecular variation in pre-eclampsia placenta based on micro-Raman spectroscopy. METHODS: Five pregnant women with pre-eclampsia from Nanfang hospital were selected as study group whose average age is 28.5 years and 38 ± 2 weeks gestation. The same period of healthy pregnant women, whose average age is 27.6 years and pregnant 39 ± 1 weeks, as control group (n = 5). The normal and pre-eclamptic placental tissues are detected by micro-Raman spectroscopy with the spectrum resolution of 1 cm(-1). RESULTS: We find that the protein structure of α-helix, ß-pleated sheet and ß-turn is overlying in pre-eclamptic placenta, which lead to a disorder of protein structure. The Raman peaks assigned to tryptophan indole ring and phenylalanine in pre-eclamptic placental tissue are more higher than that in normal tissue. CONCLUSIONS: Results suggest that the ordered structures of the main chain in protein molecules are reduced significantly, and the amino acid of side chains is damaged obviously. And a principal component analysis is used to classify the Raman spectra between normal and pre-eclamptic placental tissues. This study presents that Raman spectroscopy has a great potential on the mechanism research and diagnosis of placental lesions.
Assuntos
Placenta/química , Pré-Eclâmpsia/diagnóstico , Proteínas da Gravidez/genética , Análise Espectral Raman/métodos , Adulto , Estudos de Casos e Controles , Feminino , Variação Genética , Idade Gestacional , Humanos , Gravidez , Proteínas da Gravidez/metabolismo , Análise de Componente PrincipalRESUMO
Second harmonic microscopic imaging and spectroscopy technology has become a powerful tool for biomedical studies, especially in cancer research. In this paper, second harmonic generation in benign prostatic hyperplasia (BPH) and prostate cancer (PC) tissues in mouse model (C57BL6) have been reported. Excitated samples with different wavelength near-infrared laser from 780 to 850 nm we found that second harmonic signals from BPH nuclei stronger than that from PC, and a wavelength sensitivity was also observed in this experiment. Providing useful help for prostate malignancy diagnosis and identifying tissue components on clinic.
Assuntos
Hiperplasia/patologia , Microscopia/métodos , Imagem Óptica/métodos , Próstata/química , Próstata/patologia , Neoplasias da Próstata/patologia , Análise Espectral/métodos , Animais , Testes Diagnósticos de Rotina/métodos , Modelos Animais de Doenças , Masculino , Camundongos Endogâmicos C57BL , Patologia Cirúrgica/métodosRESUMO
Confocal micro-Raman spectroscopy is employed to study the diffusion process of dimethyl sulfoxide (DMSO) in porcine skin optical clearing. The variation of DMSO concentration with time at different depths of the skin was obtained and then the DMSO diffusion coefficient with the passive diffusion model was calculated. Results show that it has a significant difference at different depths of the skin. Also, the DMSO concentration with the depth at different times was obtained and the same method was used to find the change law of the DMSO diffusion coefficient. Results indicate that it also changes with the treatment time. The experimental results are consistent with the theoretical model in a previous study. The current results demonstrate that Raman spectroscopy has the ability to quantitatively monitor the process of optical clearing.
