Taxonomic review of the genus Compsiluroides Mesnil (Diptera: Tachinidae), with the description of a new species.

Compsiluroides communis Mesnil, C. flavipalpis Mesnil and C. meifengensis sp. nov. are redescribed and described with illustrations. Compsiluroides proboscis Chao Sun is treated as a junior synonym of C. communis. Variations of structures of the male postabdomen of C. communis are illustrated. The genus is newly recorded from Nepal and Taiwan. A key to the three known species of Compsiluroides is provided and their diagnostic characters are illustrated.

A new species of Medinodexia Townsend (Diptera: Tachinidae) from Japan, with a discussion on phylogenetic implications of the female postabdomen in Blondeliini.

Medinodexia japonica sp. nov. is described from Japan and an adult leaf beetle, Aulacophora nigripennis Motschulsky (Coleoptera: Chrysomelidae), is recorded as its host. A piercing-type ovipositor is one of the characteristics of the genus Medinodexia Townsend, but it is also found in other blondeliine genera. To evaluate the structural differences of the piercing-type ovipositor, the female postabdominal characters were examined within Blondeliini and their phylogenetic implications are briefly discussed. Females of Medinodexia are similar to those of Medina Robineau-Desvoidy for the presence of invaginations on tergite 6 and sternite 6 of the abdomen. Medinodexia exigua Shima and M. orientalis Shima are not treated here, because they are considered to belong to an undescribed genus due to differences in the male and female postabdominal characters distinguishing them from the remaining species of Medinodexia.

Androgen response element of the glycine N-methyltransferase gene is located in the coding region of its first exon.

Androgen plays an important role in the pathogenesis of PCa (prostate cancer). Previously, we identified GNMT (glycine N-methyltransferase) as a tumour susceptibility gene and characterized its promoter region. Besides, its enzymatic product-sarcosine has been recognized as a marker for prognosis of PCa. The goals of this study were to determine whether GNMT is regulated by androgen and to map its AREs (androgen response elements). Real-time PCR analyses showed that R1881, a synthetic AR (androgen receptor) agonist induced GNMT expression in AR-positive LNCaP cells, but not in AR-negative DU145 cells. In silico prediction showed that there are four putative AREs in GNMT-ARE1, ARE2 and ARE3 are located in the intron 1 and ARE4 is in the intron 2. Consensus ARE motif deduced from published AREs was used to identify the fifth ARE-ARE5 in the coding region of exon 1. Luciferase reporter assay found that only ARE5 mediated the transcriptional activation of R1881. ARE3 overlaps with a YY1 [Yin and Yang 1 (motif (CaCCATGTT, +1118/+1126)] that was further confirmed by antibody supershift and ChIP (chromatin immunoprecipitation) assays. EMSA (electrophoretic mobility shift assay) and ChIP assay confirmed that AR interacts with ARE5 in vitro and in vivo. In summary, GNMT is an AR-targeted gene with its functional ARE located at +19/+33 of the first exon. These results are valuable for the study of the influence of androgen on the gene expression of GNMT especially in the pathogenesis of cancer.