Targeting lncRNA PSMA3-AS1, a Prognostic Marker, Suppresses Malignant Progression of Oral Squamous Cell Carcinoma.

OBJECTIVE: Oral squamous cell carcinoma (OSCC) represents the most common maxillofacial malignancy. This study elucidated the clinicopathological value and molecular mechanisms of PSMA3 antisense RNA 1 (PSMA3-AS1) in OSCC. METHODS: Totally, 135 OSCC patients were recruited. PSMA3-AS1 expression and its prognostic value were assessed in this cohort. si-PSMA3-AS1 was transfected into HN4 and CAL-27 OSCC cells. Then, cell proliferation was evaluated by CCK-8, colony formation, and EdU staining. Migration and invasion were investigated through wound healing, transwell, and western blot. The PSMA3-AS1/miR-136-5p and miR-136-5p/FN1 interactions were validated by dual luciferase report, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot. RESULTS: PSMA3-AS1 upregulation was determined in OSCC tissues. The upregulation indicated pessimistic patients' outcomes. Multivariate Cox regression analyses confirmed PSMA3-AS1 as an independent prognostic indicator. Its upregulation was also found in OSCC cells. Under transfection with si-PSMA3-AS1, proliferation, migration, and invasion were all restrained in HN4 and CAL-27 OSCC cells. Furthermore, its knockdown induced the increase in E-cadherin expression and the reduction in N-cadherin and Vimentin expression. PSMA3-AS1 was a sponge of miR-136-5p. Mutual inhibition was found between two and the interactions were confirmed by dual luciferase report. It was confirmed that FN1 was a target of miR-136-5p. FN1 expression was increased by miR-136-5p inhibitors, which was lessened by si-PSMA3-AS1 cotransfection. CONCLUSION: Collectively, PSMA3-AS1 as a risk factor facilitated malignant behaviors of OSCC cells, related to the miR-136-5p/FN1 axis. Hence, PSMA3-AS1 as a potential therapeutic target for OSCC deserved further exploration.

Circular RNA FNDC3B Protects Oral Squamous Cell Carcinoma Cells From Ferroptosis and Contributes to the Malignant Progression by Regulating miR-520d-5p/SLC7A11 Axis.

Oral squamous cell carcinoma (OSCC) is a common head and neck malignancy with increasing mortality and high recurrence. Ferroptosis is an emerging programed cell death and plays an essential role in tumorigenesis. Circular RNAs (circRNAs) have been reported as a type of critical regulators in OSCC development. In this study, we identified the function of circular RNA FNDC3B (circFNDC3B) in regulating ferroptosis during the malignant progression of OSCC. Our data demonstrated that the silencing of circFNDC3B by shRNA inhibited GPX4 and SLC7A11 expression and enhanced ROS, iron, and Fe2+ levels in OSCC cells. CircFNDC3B knockdown reinforced erastin-induced inhibitory effect on OSCC cells. The depletion of circFNDC3B repressed cell proliferation and enhanced cell apoptosis of OSCC cells. Mechanically, circFNDC3B was able to increase SLC7A11 by targeting miR-520d-5p. The overexpression of SLC7A11 reversed circFNDC3B depletion or miR-520d-5p-induced ferroptosis phenotypes of OSCC cells. Moreover, tumorgenicity assays in nude mice showed that the depletion of circFNDC3B repressed OSCC cell growth in vivo. Taken together, we concluded that circFNDC3B attenuated ferroptosis of OSCC cells and contributed to OSCC progression by regulating the miR-520d-5p/SLC7A11 axis. CircFNDC3B, miR-520d-5p, and SLC7A11 may serve as potential therapeutic targets of OSCC.

An information diffusion technique to assess integrated hazard risks.

An integrated risk is a scene in the future associated with some adverse incident caused by multiple hazards. An integrated probability risk is the expected value of disaster. Due to the difficulty of assessing an integrated probability risk with a small sample, weighting methods and copulas are employed to avoid this obstacle. To resolve the problem, in this paper, we develop the information diffusion technique to construct a joint probability distribution and a vulnerability surface. Then, an integrated risk can be directly assessed by using a small sample. A case of an integrated risk caused by flood and earthquake is given to show how the suggested technique is used to assess the integrated risk of annual property loss.

[Experimental study of temperature sensor in temperature-guided radiofrequency catheter ablation].

In order to optimize temperature monitoring, the mean of temperature measured by Copper-CuNi thermocouple (TC) was compared with the actual temperature. By the use of the temperature response curve of TC, the data from monitoring temperature were analyzed in regard to the depth of installation, the diameter of TC hole and the material of ablation electrode. The accuracy and real-time of TC with a depth of 3.5 mm were better than those of 2.5 mm or 3 mm, when TC was installed in the ablation electrode TC hole. However, the difference of real-time performance was not obvious. When TC was installed in different TC holes with diameter of 0.44 mm, 0.42 mm, 0.33 mm respectively, TC with the diameter of 0.33 mm TC hole was noted to be of higher accuracy and better real-time to response temperature, compared with others. In terms of material quality, the slope of platinum temperature response curve was greater than that of stainless steel, while the accuracy of sensor temperature changed inconspicuously. As a result, the monitoring device of temperature should be put to the heat source as nearly as possible. Also, platinum ablation electrode with better thermal conductivity should be chosen.