RESUMO
Recombinant expression vector was constructed by techniques of gene recombination, and identified by restriction endonuclease and sequence analysis. Then the recombinant was transfected into B16 cell by techniques of gene transfection and expressions were detected by RT-PCR and IFA. After that, transfected cells were inoculated into subcutaneous of mouse and the forming tumor and expression of HPV16L1 protein after tumor was formed was observed. Identification of pcDNA- HPV16L1 by enzyme digestion showed that the length, inserted location and direction of the target gene which was inserted into the recombinant was correct and the expression of L1 in transfected cell was observed by IFA. The inoculated cells could form tumor in vivo obviously and HPV16 L1 protein could express in the cells stably.
