RESUMO
Gen-Probe's DNA probes were evaluated for use in the identification of clinical isolates of Histoplasma capsulatum var. capsulatum and Cryptococcus neoformans. Ninety-five mould-phase fungi were probed, including 41 isolates of H. capsulatum var. capsulatum. Similarly, 98 yeasts, including 42 C. neoformans isolates, were examined by using the C. neoformans DNA probe. In the study, both probes demonstrated 100% specificity and 100% sensitivity. Their use in the clinical laboratory may significantly reduce the time required for definitive identification of fungi.
Assuntos
Cryptococcus neoformans/genética , Sondas de DNA , Histoplasma/genética , Técnicas de Sonda Molecular , Criptococose/diagnóstico , Cryptococcus neoformans/isolamento & purificação , DNA Fúngico/genética , Estudos de Avaliação como Assunto , Histoplasma/isolamento & purificação , Histoplasmose/diagnóstico , Humanos , Técnicas de Sonda Molecular/estatística & dados numéricos , Sensibilidade e EspecificidadeRESUMO
Apophysomyces elegans was isolated from the subcutaneous tissue, muscle and bone of a patient who had fallen from a height of 55 feet. Broad, sparsely septate hyphae were present in the tissue. Surgical debridement of wounds and amphotericin B treatment were not sufficient in controlling rapid tissue necrosis. Ultimately, amputation of the two affected limbs was necessary.
