RESUMO
OBJECTIVES: To evaluate long-term changes in acetylcholinesterase (AChE) activity in CSF and blood following donepezil treatment in relation to the concentration of donepezil and cognition in AD patients. METHODS: CSF or blood (or both) samples of a total of 104 patients with mild AD were used [MMSE score 23 +/- 0.4; age 75 +/- 1 years (mean +/- SEM); n=53 for CSF and n=51 for plasma/red blood cell (RBC) samples]. The patients were treated with 5 or 10 mg/day donepezil and clinically followed for 2 years. The CSF and RBC AChE activities were measured by the Ellman's direct colorimetric assay. Protein levels of two variants of AChE ("read-through" AChE-R and synaptic AChE-S) were determined by an ELISA-like method. RESULTS: The plasma donepezil concentration was dose-dependent (between 30 and 60 ng/mL in the 5-mg and 10-mg group, respectively). The CSF donepezil concentration was 10 times lower than the plasma level and showed dose- and time-dependent kinetics. The RBC AChE inhibition was moderate (19-29%). CSF AChE-S inhibition was estimated to 30-40% in the 5-mg and 45-55% in the 10-mg group. Positive correlations were observed between the CSF AChE inhibition, an increased protein level of the AChE-R variant and MMSE examination. Patients with high AChE inhibition (>or=45%) showed a stabilized MMSE test result after up to two years, while a significant decline was observed in AD patients with lower AChE inhibition (Assuntos
Acetilcolinesterase/efeitos dos fármacos
, Doença de Alzheimer/tratamento farmacológico
, Inibidores da Colinesterase/uso terapêutico
, Cognição/efeitos dos fármacos
, Indanos/uso terapêutico
, Piperidinas/uso terapêutico
, Acetilcolinesterase/líquido cefalorraquidiano
, Idoso
, Doença de Alzheimer/líquido cefalorraquidiano
, Inibidores da Colinesterase/análise
, Cromatografia Líquida de Alta Pressão
, Donepezila
, Relação Dose-Resposta a Droga
, Eritrócitos/efeitos dos fármacos
, Eritrócitos/enzimologia
, Feminino
, Humanos
, Indanos/análise
, Masculino
, Piperidinas/análise
, Fatores de Tempo