RESUMO
This study aimed to examine the effects of the recombinant human somatotropin (rhGH) on protecting neuronal function, and improving learning and memory deficits in mice. Mice were intracerebroventricularly (icv) injected with the aggregated amyloid beta-peptide (Abeta) to mimic the Alzheimer's disease (AD). The learning and memory functions in mice were examined by the step through test (an index of long-term memory) and the water maze performance (an index of spatial recognition memory). The results indicated that the mice treated with rhGH showed significant reduction of the error counts and the long memory retentions in the step-through test, and short swimming times in the water maze performance. Toxic effects of free radicals, damages of cholinergic neurons, and increased lipid peroxidation appeared in the cerebra of Abeta-treated mice, manifesting an increase of malondialdehyde (MDA) and decline of glutathione (GSH) level, an increment of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities, and a reduction of the acetylcholine (ACh) level. The gel electrophoresis pattern of the cerebra of mice treated with Abeta showed a typical DNA ladder of apoptosis. The in vivo experiments showed that the rhGH treatment significantly reversed the elevated MDA, ChAT, AChE, and the decreased GSH, ACh levels in the Abeta model mice. The results suggested that there were potential uses of the neuroprotective action of rhGH in the remedy of AD.
Assuntos
Peptídeos beta-Amiloides , Citoproteção/efeitos dos fármacos , Hormônio do Crescimento Humano/farmacologia , Hormônio do Crescimento Humano/uso terapêutico , Deficiências da Aprendizagem/induzido quimicamente , Deficiências da Aprendizagem/prevenção & controle , Transtornos da Memória/induzido quimicamente , Transtornos da Memória/prevenção & controle , Doença de Alzheimer/induzido quimicamente , Doença de Alzheimer/prevenção & controle , Animais , Apoptose/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Camundongos , Fragmentos de Peptídeos , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêuticoRESUMO
<p><b>OBJECTIVE</b>To explore the utility of Principal Factor Analysis (PFA) in chromatographic data for quality control.</p><p><b>METHOD</b>Chromatographic fingerprints of processed root pieces of Paeonia lactiflora were determined by HPLC, the PFA was used for data processing.</p><p><b>RESULT</b>The quantitative differences among different growing areas and different processing batches were found with the method.</p><p><b>CONCLUSION</b>The method could be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.</p>
Assuntos
Cromatografia Líquida de Alta Pressão , Métodos , Ecossistema , Análise Fatorial , Temperatura Alta , Pinellia , Química , Preparações de Plantas , Química , Padrões de Referência , Raízes de Plantas , Química , Plantas Medicinais , Química , Controle de Qualidade , Reprodutibilidade dos Testes , Tecnologia Farmacêutica , Métodos , VinhoRESUMO
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.</p><p><b>METHOD</b>The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).</p><p><b>RESULT</b>Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).</p><p><b>CONCLUSION</b>The method could be used for quality control of Bile Arisaema.</p>
Assuntos
Animais , Arisaema , Química , Bile , Química , Cromatografia Líquida de Alta Pressão , Métodos , Ácido Desoxicólico , Combinação de Medicamentos , Temperatura Alta , Luz , Medicina Tradicional Chinesa , Padrões de Referência , Tubérculos , Química , Plantas Medicinais , Química , Controle de Qualidade , Reprodutibilidade dos Testes , Espalhamento de RadiaçãoRESUMO
<p><b>OBJECTIVE</b>To provide the experimental data for the quality control of Paeonia lactiflora a prepared Chinese medicinal herb.</p><p><b>METHOD</b>The contents paeoniflorin among different growing areas and different processing methods methods were assayed by HPLC.</p><p><b>RESULT</b>The quantitative differences of paeoniflorin content among different growing areas and different processing methods wereanalyzed quantitatively.</p><p><b>CONCLUSION</b>The quantitative differences among different growing areas were not significant and the quantitative differences among different processing methods within 10%.</p>
Assuntos
Benzoatos , Hidrocarbonetos Aromáticos com Pontes , China , Cromatografia Líquida de Alta Pressão , Ecossistema , Glucosídeos , Temperatura Alta , Monoterpenos , Paeonia , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Controle de Qualidade , Tecnologia Farmacêutica , Métodos , VinhoRESUMO
<p><b>OBJECTIVE</b>To provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine.</p><p><b>METHOD</b>Traditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed a nanalysed.</p><p><b>RESULT AND CONCLUSION</b>The controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.</p>
