Genetic diversity of Histoplasma and Sporothrix complexes based on sequences of their ITS1-5.8S-ITS2 regions from the BOLD System / Diversidad genética de los complejos Histoplasma y Sporothrix en función de las secuencias de sus regiones ITS1-5.8S-ITS2 del BOLD System

High sensitivity and specificity of molecular biology techniques have proven usefulness for the detection, identification and typing of different pathogens. The ITS (Internal Transcribed Spacer) regions of the ribosomal DNA are highly conserved non-coding regions, and have been widely used in different studies including the determination of the genetic diversity of human fungal pathogens. This article wants to contribute to the understanding of the intra- and interspecific genetic diversity of isolates of the Histoplasma capsulatum and Sporothrix schenckii species complexes by an analysis of the available sequences of the ITS regions from different sequence databases. ITS1-5.8S-ITS2 sequences of each fungus, either deposited in GenBank, or from our research groups (registered in the Fungi Barcode of Life Database), were analyzed using the maximum likelihood (ML) method. ML analysis of the ITS sequences discriminated isolates from distant geographic origins and particular wild hosts, depending on the fungal species analyzed. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012 (AU)

Las técnicas de biología molecular han proporcionado instrumentos de alta sensibilidad y especificidad, útiles para la detección, identificación y tipificación de diferentes patógenos. Las regiones ITS (Internal Transcribed Spacer) del ADN ribosómico están altamente conservadas y no son codificantes. Estas regiones se han utilizado ampliamente en diferentes tipos de estudios, incluida la determinación de la diversidad genética de hongos patógenos del ser humano. La finalidad de este artículo es contribuir al conocimiento de la diversidad genética intra- e interespecífica de aislamientos de los complejos de Histoplasma capsulatum y Sporothrix schenckii a través del análisis de las secuencias disponibles de las regiones ITS en distintos bancos de secuencias. Las secuencias de las regiones ITS1-5.8S-ITS2, de cada hongo, depositadas en el GenBank, junto con las obtenidas por nuestros grupos de investigación (depositadas en la Fungal Barcoding of Life Database), se analizaron con el método de máxima probabilidad (ML, por sus siglas en inglés). El análisis ML de las secuencias de las regiones ITS discriminó aislamientos de orígenes geográficos distantes y de huéspedes salvajes particulares, de acuerdo con la especie fúngica analizada.Este artículo forma parte de una serie de estudios presentados en el «V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi» (Oaxaca, México, 2012) (AU)

Genetic diversity of Histoplasma and Sporothrix complexes based on sequences of their ITS1-5.8S-ITS2 regions from the BOLD System.

High sensitivity and specificity of molecular biology techniques have proven usefulness for the detection, identification and typing of different pathogens. The ITS (Internal Transcribed Spacer) regions of the ribosomal DNA are highly conserved non-coding regions, and have been widely used in different studies including the determination of the genetic diversity of human fungal pathogens. This article wants to contribute to the understanding of the intra- and interspecific genetic diversity of isolates of the Histoplasma capsulatum and Sporothrix schenckii species complexes by an analysis of the available sequences of the ITS regions from different sequence databases. ITS1-5.8S-ITS2 sequences of each fungus, either deposited in GenBank, or from our research groups (registered in the Fungi Barcode of Life Database), were analyzed using the maximum likelihood (ML) method. ML analysis of the ITS sequences discriminated isolates from distant geographic origins and particular wild hosts, depending on the fungal species analyzed. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012).