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1.
J Adv Model Earth Syst ; 11(7): 2130-2162, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33101595

RESUMO

Peatlands are poorly represented in global Earth system modeling frameworks. Here we add a peatland-specific land surface hydrology module (PEAT-CLSM) to the Catchment Land Surface Model (CLSM) of the NASA Goddard Earth Observing System (GEOS) framework. The amended TOPMODEL approach of the original CLSM that uses topography characteristics to model catchment processes is discarded, and a peatland-specific model concept is realized in its place. To facilitate its utilization in operational GEOS efforts, PEAT-CLSM uses the basic structure of CLSM and the same global input data. Parameters used in PEAT-CLSM are based on literature data. A suite of CLSM and PEAT-CLSM simulations for peatland areas between 40°N and 75°N is presented and evaluated against a newly compiled data set of groundwater table depth and eddy covariance observations of latent and sensible heat fluxes in natural and seminatural peatlands. CLSM's simulated groundwater tables are too deep and variable, whereas PEAT-CLSM simulates a mean groundwater table depth of -0.20 m (snow-free unfrozen period) with moderate temporal fluctuations (standard deviation of 0.10 m), in significantly better agreement with in situ observations. Relative to an operational CLSM version that simply includes peat as a soil class, the temporal correlation coefficient is increased on average by 0.16 and reaches 0.64 for bogs and 0.66 for fens when driven with global atmospheric forcing data. In PEAT-CLSM, runoff is increased on average by 38% and evapotranspiration is reduced by 19%. The evapotranspiration reduction constitutes a significant improvement relative to eddy covariance measurements.

2.
Tree Physiol ; 27(1): 115-31, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17169913

RESUMO

We hypothesized that changes in net ecosystem productivity (NEP) during aging of coastal Douglas-fir (Pseudotsuga menziesii Mirb. Franco) stands could be explained by (1) changing nutrient uptake caused by different time scales for decomposition of fine, non-woody and coarse woody litter left after harvesting, (2) declines in canopy water status with lengthening of the water uptake pathway during bole and branch growth, and (3) increases in the ratio of autotrophic respiration (R (a)) to gross primary productivity (GPP) with phytomass accumulation. These hypotheses were implemented and tested in the mathematical model ecosys against eddy covariance (EC) measurements of forest CO(2) and energy exchange in a post-clearcut Douglas-fir chronosequence. Hypothesis 1 explained how (a) an initial rise in GPP observed during the first 3 years after clearcutting could be caused by nutrient mineralization from rapid decomposition of fine, non-woody litter with lower C:N ratios (assart effect), (b) a slower rise in GPP during the next 20 years could be caused by immobilization during later decomposition of coarse woody litter, and (c) a rapid rise in GPP between 20 and 40 years after clearcutting could be caused by nutrient mineralization with further decomposition of coarse woody litter and of its decomposition products. During periods (a) and (b), heterotrophic respiration (R (h)) from decomposition of fine and coarse litter greatly exceeded net primary productivity (NPP = GPP - R (a)) so that Douglas-fir stands were large sources of CO(2). During period (c), NPP exceeded R (h) so that these stands became large sinks for CO(2). Hypothesis 2 explained how declines in NPP during later growth in period (c) could be caused by lower hydraulic conductances in taller trees that would force lower canopy water potentials and hence greater sensitivity of stomatal conductances and CO(2) uptake to vapor pressure deficits. Enhanced sensitivity to vapor pressure deficits was also apparent in the EC measurements over the post-clearcut chronosequence. Hypothesis 3 did not contribute to the explanation of forest age effects on NEP.


Assuntos
Abies/fisiologia , Ecossistema , Agricultura Florestal/métodos , Modelos Biológicos , Árvores/fisiologia , Simulação por Computador , Nitrogênio/metabolismo , Temperatura , Fatores de Tempo , Água/metabolismo
3.
Exp Hematol ; 21(9): 1227-32, 1993 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7687218

RESUMO

This paper analyzes the function of mouse granulocytes in the long term, after external irradiation with x- and gamma-rays and 239Pu contamination at different gestational ages and in a variety of culture conditions. These treatments can produce persistent defects in the stroma, which regulates hematopoiesis. Superoxide-anion production has been measured in granulocytes from peripheral blood and from long-term bone marrow cultures (LTBMC). A significant enhancement of O2- is produced using single or fractionated doses of x-rays; however, little or no increase is observed with gamma-rays. With 239Pu, enhancement of O2- depends on gestational age at contamination. The absence of hydrocortisone (HC) in LTBMC and the irradiation of the adherent layer with 15 Gy stimulate O2- production. The increased production of O2- appears to be correlated with an excess of colony-stimulating factors (CSFs) released to the supernatant by stromal cells. Neutralization with anti-granulocyte-macrophage CSF (anti-GM-CSF) monoclonal antibody shows that GM-CSF is the main factor produced. In summary, conditions that lead to residual stromal damage also result in the generation of granulocytes that are functionally primed for excess superoxide-anion production.


Assuntos
Granulócitos/fisiologia , Irradiação Corporal Total , Animais , Células da Medula Óssea , Células Cultivadas , Feminino , Fator Estimulador de Colônias de Granulócitos/farmacologia , Granulócitos/efeitos da radiação , Macrófagos/citologia , Masculino , Camundongos , Camundongos Endogâmicos , Plutônio/farmacologia , Proteínas Recombinantes/farmacologia , Células-Tronco , Superóxidos/metabolismo
4.
Int J Radiat Biol ; 64(2): 231-5, 1993 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-8103548

RESUMO

Four groups of 400 12-week-old CBA/H mice were injected i.p. with 69, 139, 280 and 550 Bqg-1 224Ra. A further group of 400 mice were injected i.p. with diluting solution only. The mice were then allowed unrestricted access to food and water until they died or were killed. 53 cases of myeloid leukaemia and 22 cases of osteosarcoma were confirmed in the 2000 mice injected, and for both tumour types direct relationships were shown to exist between the amount of 224Ra administered and the incidence of tumours. It is concluded that mouse is at a greater risk from myeloid leukaemia than from osteosarcoma in the region of administered 224Ra below that which causes a maximum yield of osteosarcoma. These results are discussed in the light of the present acceptance of osteosarcoma as the major risk to man from bone-seeking alpha-particle emitters.


Assuntos
Leucemia Mieloide/etiologia , Neoplasias Induzidas por Radiação , Osteossarcoma/etiologia , Rádio (Elemento)/toxicidade , Animais , Masculino , Camundongos , Camundongos Endogâmicos CBA
5.
Int J Radiat Biol ; 61(3): 393-403, 1992 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-1347073

RESUMO

The distribution of 239Pu in various tissues of foetal and postnatal offspring of pregnant mice, injected i.v. at 13 days gestation with 30 kBq 239Pu/kg (in some cases with 10 or 100 kBq/kg), together with the numbers of haemopoietic progenitors in the bone marrow, spleen and liver, were measured through to 1 year post-partum. The quality of the haemopoietic microenvironment in these mice was also measured using the renal-capsule implant method. The largest radiation dose received by any haemopoietic organ was that in the liver, amounting to 10-14 mGy, as reported previously. In spite of normal numbers of haemopoietic spleen colony-forming cells (CFC-S) in the liver and seeding, at birth, into the bone marrow where the level of plutonium was minimal, a long-term deficit in their number rapidly developed. The development of the stromal microenvironment, however, was also deficient, suggesting that the dose of alpha-irradiation to the foetal liver was sufficient to cause sublethal damage in those cells destined to become the precursors of the supportive haemopoietic microenvironment in bone marrow and spleen. The results of this study suggest that although the placenta affords significant shielding to the tissues of the developing foetus from maternal contamination, the long-term effects on haemopoiesis are comparable to those in mice contaminated as adults. This further implies that the developing haemopoietic tissues are exquisitely sensitive to 239Pu contamination.


Assuntos
Feto/efeitos da radiação , Sistema Hematopoético/efeitos da radiação , Troca Materno-Fetal , Plutônio/administração & dosagem , Efeitos Tardios da Exposição Pré-Natal , Partículas alfa , Animais , Feminino , Camundongos , Gravidez , Útero
7.
Int J Radiat Biol ; 59(2): 467-78, 1991 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-1671696

RESUMO

Pregnant mice (at 13 days gestation) and age-matched controls were injected with 30 kBq 239Pu/kg and the distribution of plutonium in maternal and foetal tissues measured. Approximately 2% of the activity injected into the mother reached each foetus in 24 h, 95% of which was contained in membranes and placenta. The concentration of plutonium in foetal liver was 3 times the average foetal body concentration; both liver and body concentrations in the foetus increased by the end of gestation. Each pup accumulated only 0.01% extra injected activity after 9 days lactation and, as the resulting concentrations in the neonatal skeleton were low, we conclude that the greatest haemopoietic risk to the offspring from mid-term contamination in utero is in the foetal liver (which received an average dose of 10-14 mGy between the time of mid-term contamination and birth). By the end of gestation about one-quarter of the original activity was transferred to foetal tissues from the maternal liver and skeleton. No significant changes in maternal distribution were detected as a result of lactation. The results of this study are discussed, along with a compilation of previously published data.


Assuntos
Animais Recém-Nascidos/metabolismo , Lactação/metabolismo , Troca Materno-Fetal , Plutônio/farmacocinética , Prenhez/metabolismo , Animais , Feminino , Injeções Intravenosas , Camundongos , Camundongos Endogâmicos C57BL , Plutônio/administração & dosagem , Gravidez , Distribuição Tecidual
8.
Int J Radiat Biol ; 59(1): 211-27, 1991 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-1671068

RESUMO

Experiments are described which investigate the long-term damage to haemopoietic progenitor cells (CFU-S) and their microenvironment in mouse marrow resulting from the administration of leukaemogenic amounts of plutonium-239 and radium-224. 239Pu (35 Bq g-1 body weight) and 224Ra (555 Bg g-1 body weight) were injected into 10-12-week-old mice, and numbers, proliferative activity and self-renewal capacity of CFU-S were measured at different locations in femoral marrow at intervals over the following 2 years. Parallel measurements were also made of the quality of the haemopoietic microenvironment by ectopic transplantation of bone marrow cells. There was some recovery from the initial effects of 239Pu on CFU-S numbers after 3-6 months, although the recovery was not maintained in all marrow fractions. Following 224Ra administration there was an initial transient increase in CFU-S numbers in the fraction of marrow furthest from bone surfaces but a considerable depression in numbers in other regions of marrow; there was no recovery between 3 and 6 months and subsequent recovery was not complete in all regions of marrow. The differential responses of CFU-S and the haemopoietic microenvironment following 224Ra or 239Pu administration seemed in some ways related to the metabolism of the radionuclides. There was a profound reduction in the ability of marrow to generate ossicles when transplanted under the kidney capsule as a result of the administration of either 224Ra or 239Pu, with only transient recoveries from the effects of 239Pu at 4 days and at 3 months after injection.


Assuntos
Medula Óssea/efeitos da radiação , Células-Tronco Hematopoéticas/efeitos da radiação , Plutônio , Rádio (Elemento) , Animais , Células da Medula Óssea , Injeções Intraperitoneais , Injeções Intravenosas , Masculino , Camundongos , Camundongos Endogâmicos CBA , Plutônio/administração & dosagem , Rádio (Elemento)/administração & dosagem , Fatores de Tempo
10.
Exp Hematol ; 17(7): 836-42, 1989 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-2666146

RESUMO

Marrow from 5-fluorouracil- or cyclophosphamide-treated mice, injected into lethally irradiated recipients, gives an increasing number of spleen colonies between days 7 and 14. It has been suggested that the later-forming colonies result from the more primitive spleen colony-forming units (CFU-S), which first seed into the marrow, only later to be recirculated and form colonies in the spleen. Strontium 89 (89Sr), a bone-seeking radionuclide, was injected into recipient mice to block such putative recirculation. A dose of 89Sr, which killed at least 99.8% of CFU-S in, or entering, the bone cavities, was incapable of preventing the increase in spleen colony numbers. Similarly, the splenic environment, modified by the presence of spleen colonies and able to provide a better bed for trapping CFU-S from the peripheral circulation, yielded the same number of further CFU-S, whether or not the animal had received 89Sr. Thus, it was concluded that the 12-day CFU-S does not seed initially into the marrow spaces. Direct observation of the quality of CFU-S initially seeding into the bone marrow and spleen showed, by retransplantation into secondary irradiated mice, that a similar spectrum of CFU-S types had seeded both organs.


Assuntos
Células da Medula Óssea , Células-Tronco Hematopoéticas/citologia , Baço/citologia , Animais , Transplante de Medula Óssea , Movimento Celular , Ensaio de Unidades Formadoras de Colônias , Camundongos , Radioisótopos de Estrôncio/toxicidade , Fatores de Tempo
11.
Health Phys ; 54(6): 607-15, 1988 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-3163989

RESUMO

A method is described which allows dose calculations to be made to individual target cells in different regions of mouse bone marrow exposed to alpha particles emitted from bone. The method takes into account the variable rate of transfer of energy along the tracks of alpha particles and was applied to experiment-based values calculated for the concentration of 224Ra on bone surfaces after an injection of a leukemogenic amount of the nuclide. These calculations show a minimum dose of 11 Gy in small (less than 50-micron) marrow spaces and 10 Gy close to bone surface in the shaft of the femur. The results suggest that leukemogenic doses are likely to occur at some distance from bone surfaces in wide marrow spaces and that osteosarcoma is not likely to be induced directly in cells immediately aligning bone surfaces.


Assuntos
Osso e Ossos/efeitos da radiação , Método de Monte Carlo , Pesquisa Operacional , Monitoramento de Radiação/métodos , Rádio (Elemento)/análise , Absorção , Partículas alfa , Animais , Medula Óssea/efeitos da radiação , Células da Medula Óssea , Masculino , Camundongos , Camundongos Endogâmicos CBA , Modelos Biológicos , Neoplasias Induzidas por Radiação/etiologia , Osteossarcoma/etiologia , Doses de Radiação , Radioatividade , Rádio (Elemento)/toxicidade , Fatores de Tempo
12.
Artigo em Inglês | MEDLINE | ID: mdl-3493993

RESUMO

Plutonium-239 was injected into 12-week-old female CBA/H mice in the range 1.85-18.5 kBq kg-1 either as a single injection or as 16 injections spaced at 3.5 day intervals over eight weeks. There was a highly significant increase in the yield of fully developed osteosarcomas with increased amounts of 239Pu for both modes of injection. Osteosarcomas too small to be diagnosed radiographically were also seen in many bones and small but significant yields of myeloid leukaemia were seen in animals given plutonium. Although more myeloid leukaemia was seen in the mice given plutonium in divided amounts than in those given the plutonium in a single injection it could not be shown that multiple injection significantly affected the yield of either late effect.


Assuntos
Leucemia Induzida por Radiação/etiologia , Neoplasias Induzidas por Radiação/etiologia , Plutônio , Sarcoma Experimental/etiologia , Animais , Relação Dose-Resposta à Radiação , Feminino , Camundongos , Camundongos Endogâmicos CBA , Neoplasias Induzidas por Radiação/patologia , Osteossarcoma/etiologia , Osteossarcoma/patologia , Sarcoma Experimental/patologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-3489686

RESUMO

Calculations are described, based on experimental findings, which show the variation of absorbed dose from 224Ra in bone marrow of CBA/H mice. These calculations indicate that, following an injection of a leukaemogenic amount of 16 kBq 224Ra into these mice, most marrow cells in the cancellous bone of femur ends are killed but most marrow cells in the femur shaft survive. The calculations also suggest that the mean leukaemogenic absorbed dose of about 1.5 Gy is received by a population of marrow cells about 30 microns from bone surface in the femur shaft.


Assuntos
Osso e Ossos , Rádio (Elemento) , Animais , Medula Óssea , Osso e Ossos/metabolismo , Injeções Intravenosas , Camundongos , Doses de Radiação , Rádio (Elemento)/administração & dosagem , Rádio (Elemento)/metabolismo
14.
Artigo em Inglês | MEDLINE | ID: mdl-3486845

RESUMO

Mice were injected with plutonium-239 (960 Bq/mouse) and, over a period of four months, the response of haemopoietic tissue and the self-renewal capacity of its stem cells was monitored. Cellularity, CFU-S concentration and self-renewal capacity were measured in five different regions of bone and marrow--axial and marginal marrow of the femoral shaft, femur shaft, proximal and distal ends of the femur shaft and vertebrae. Cellularities were little affected by plutonium but CFU-S were reduced in all regions, most severely in the bone shaft and marginal marrow due to the initial deposition of plutonium on the bone surface, by four days. The reduction in axial CFU-S, however, was due probably to a relatively long plasma half-life resulting from the tendency of plutonium to combine with plasma proteins. The capacity of CFU-S for self-renewal was reduced and remained low in all zones. Thus, although the highly self-renewing axial CFU-S were depleted, and remained so, due probably to a longer term redistribution of plutonium throughout the marrow, additional proliferation of the more mature CFU-S in the other zones kept their self-renewal low while replenishing their numbers and maintaining a normal cell output.


Assuntos
Células-Tronco Hematopoéticas/efeitos da radiação , Plutônio , Partículas alfa , Animais , Ensaio de Unidades Formadoras de Colônias , Feminino , Fêmur/efeitos da radiação , Vértebras Lombares/efeitos da radiação , Masculino , Camundongos
15.
Artigo em Inglês | MEDLINE | ID: mdl-3872276

RESUMO

Radium-224 was injected into 12-week-old male CBA mice in the range 2-64 kBq per mouse either as a single injection or as eight injections spaced at 3.5 day intervals over 4 weeks. Small but significant yields of myeloid leukaemia or osteosarcoma were obtained in all but the control groups. An effect of mode of administration (single or multiple injections) could not be demonstrated but the combined results showed: a maximum yield of myeloid leukaemia in the region 8-16 kBq 224Ra; a greater yield of osteosarcoma than myeloid leukaemia at 64 kBq 224 Ra injected.


Assuntos
Leucemia Mieloide/etiologia , Leucemia Induzida por Radiação/etiologia , Neoplasias Induzidas por Radiação/etiologia , Osteossarcoma/etiologia , Rádio (Elemento) , Animais , Masculino , Camundongos , Camundongos Endogâmicos CBA
16.
Arch Oral Biol ; 30(1): 55-64, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-3857886

RESUMO

A high incidence of incisor abnormalities was found in aged control and aged 224Ra-treated male CBA mice. Visual examination of the abnormalities in both controls and treated mice revealed extreme shortening of the upper incisors and hypoplastic, grooved or undulating enamel. The administration of 865 or 1730 nCi of 224Ra hastened the onset of incisor abnormalities although no specific feature was attributable solely to radium toxicity. Radiography and histology revealed corrugated incisors, obliteration of the pulp cavity, extension and disorganized growth of incisors basally, secondary incisors, open pulp and fractures within the alveoli. There was a statistically-significant reduction in the number of molars present in animals given 432, 865 or 1730 nCi 224Ra.


Assuntos
Envelhecimento/efeitos da radiação , Incisivo/efeitos da radiação , Lesões Experimentais por Radiação/etiologia , Rádio (Elemento)/efeitos adversos , Animais , Esmalte Dentário/efeitos da radiação , Incisivo/diagnóstico por imagem , Incisivo/patologia , Masculino , Camundongos , Camundongos Endogâmicos CBA , Lesões Experimentais por Radiação/diagnóstico por imagem , Lesões Experimentais por Radiação/patologia , Radiografia , Tório , Fatores de Tempo
19.
Calcif Tissue Int ; 34(4): 416-21, 1982 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-6814731

RESUMO

The rate at which blood is supplied to several bones in female CBA mice was calculated from 18F measurements in bone and blood. Blood flow measurements were compared with plutonium uptake in whole bone and on endosteal and periosteal bone surfaces. The results showed that: (a) the rate at which blood is supplied to bone determines the rate of deposition of plutonium; (b) there is a threshold rate of blood supply below which plutonium is not deposited; and (c) the rate of blood supply determines the density of plutonium deposition on endosteal but not no periosteal bone surfaces. These results are discussed in the light of the current bone blood supply hypotheses.


Assuntos
Osso e Ossos/irrigação sanguínea , Plutônio/metabolismo , Animais , Osso e Ossos/metabolismo , Feminino , Flúor , Camundongos , Camundongos Endogâmicos CBA , Periósteo/irrigação sanguínea , Periósteo/metabolismo , Radioisótopos
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