RESUMO
After a brief discussion of the merits and limitations of high-pressure liquid chromatography (HPLC) relative to other chromatographic methods, special problems in the application to steroids are discussed. Publications on HPLC of steroids are then discussed under the headings of individual classes, arranged generally in the order of increasing polarity.
Assuntos
Esteroides/análise , Córtex Suprarrenal/análise , Alcaloides/análise , Androstanos/análise , Animais , Ácidos e Sais Biliares/análise , Glicosídeos Cardíacos/análise , Cromatografia Líquida de Alta Pressão/métodos , Estrogênios/análise , Pregnanos/análise , Sapogeninas/análise , Esteróis/análise , Vitamina D/análiseRESUMO
The nature of Ig receptors carried by lipopolysaccharide (LPS)-stimulated mouse spleen B cells was analyzed by surface iodination, direct antiserum precipitation and polyacrylamide gel electrophoresis. LPS activation led to a rapid decrease of surface IgD to 20 % and 80 % of the original level on days three and five of culture, respectively. Efficiency of iodination of cells doubled after culture but the proportional radioactivity in IgM reace of cells, but only in small amounts (1 % of total surface Ig) and this expression of IgG remained constant during 5 days of culture. We could definitively identify gamma-chains on the surface of cells, but only in small amounts (1% of total surface Ig) and this expression of IgG remained constant during 5 days of culture.
Assuntos
Sítios de Ligação de Anticorpos , Imunoglobulina D/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Lipopolissacarídeos/farmacologia , Receptores de Antígenos de Linfócitos B/análise , Baço/imunologia , Animais , Linfócitos B/imunologia , Radioisótopos do Iodo , Linfócitos/metabolismo , Camundongos , Camundongos Endogâmicos CBARESUMO
High-pressure liquid chromatography was used to separate the following steroidal alkaloids: tomatidine, solanidine, solasodine, rubijervine, veratramine and jervine. The method was used to prepare crystalline solanidine from a crude mixture of aglycones obtained from Solanum chacoense, and to separate radioactive solanidine from extracts of potato plants fed with [4-C]cholesterol.
Assuntos
Alcaloides/análise , Cromatografia Líquida de Alta Pressão , Esteroides/análise , Métodos , Piperidinas/análise , Alcaloides de Solanáceas , Espirostanos/análise , Tomatina/análogos & derivados , Alcaloides de Veratrum/análiseRESUMO
Surface IgM on murine splenic lymphocytes was capped using a rhodamine-coupled anti-mu chain system. The cells were then reacted with a polyspecific, fluorescein-coupled anti-mouse immunoglobulin (Ig) under non-capping conditions. A large number (31-41% of total Ig-bearing cells) which gave red caps with anti-mu subsequently stained green peripherally with anti-Fab under the non-capping conditions. The remaining stained cells were divided between those showing only red caps (17-29% of Ig-bearing cells) and those showing only green rings (37-45% of Ig-bearing cells). These data are interpreted by assuming that most of the staining with the second reagent (fluorescein-coupled anti-mouse Ig) is due to the presence of surface associated IgD.
