[Effect of guizhi decoction on adenyl cyclase and phosphodiesterase in hypothalamus of rats models of fever and hypothermia].

OBJECTIVE: To observe the effect of Guizhi Decoction (GZD) on adenyl cyclase (AC) and phosphodiesterase (PDE) activities in hypothalamus of rat models of fever and hypothermia. METHODS: The AC and PDE activities in hypothalamus were determined using radio-isotope method. RESULTS: GZD could lower the AC activity in yeast induced fever rat model (P < 0.05), but cause rise of AC activity in aminopyrine induced hypothermia model (P < 0.05). No significant influence of GZD on PDE activity was shown in both fever or hypothermia models. CONCLUSION: The bi-directional thermo-regulation effect of GZD might be partially due to influence on AC activity.

[Effect and mechanism of active fraction A guizhi decoction on dual-directional thermoregulation: effect on heat shock protein in hypothalamus of rats].

OBJECTIVE: To investigate the effect of active fraction A in Guizhi Decoction (Fr.A) on dual-directional thermoregulation and its mechanism of influencing heat shock protein (HSP) in hypothalamus. METHOD: Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats. RESULTS: Regulating the body temperature in dual-direction, Fr.A could antagonize the decrease of HSP contents of hypothalamus in hypothermal rats induced by aminopyrine, and abate the HSP content in febrile rats induced by yeast. CONCLUSION: Fr.A adjusts the body temperature through regulating the contents of HSP of hypothalamus in febrile and hypothermal rats.