Outcomes of Bone Marrow Mononuclear Cell Transplantation for Neurological Sequelae Due to Intracranial Hemorrhage Incidence in the Neonatal Period: Report of Four Cases.

Aim: The aim of this study was to present primary outcomes of autologous bone marrow mononuclear cell (BMMNC) transplantation to improve neurological sequelae in four children with intracranial hemorrhage (ICH) incidence during the neonatal period. Methods: GMFM88 and modified Ashworth score were used to assess motor function and muscle spasticity before BMMNC transplantation and after transplantation. Brain MRI was performed to evaluate brain morphology before and after BMMNC transplantation. Bone marrow were harvested from anterior iliac crest puncture and BMMNCs were isolated using Ficoll gradient centrifugation. The microbiological testing, cell counting, and hematopoietic stem cell (hHSC CD34+ cell) analysis were performed, following which BMMNCs were infused intrathecally. Results: Improvement in motor function was observed in all patients after transplantation. In addition, muscle spasticity was reduced in all four patients. Conclusion: Autologous BMMNC transplantation may improve motor function and reduce muscle spasticity in children with ICH incidence during the neonatal period.

Morphological Feature of Pluronic F127 and Its Application in Burn Treatment.

The morphological features of pluronic F127 at various concentrations were investigated and 20% aqueous pluronic F127 solution was chosen for the preparation of biomembrane. The pluronic solution was mixed with appropriate concentration of excipients such as PVA, Povidon S630, PG, Nipagin, Neomycin and Panthenol. The prepared pluronic F127 was thermosensitive, a liquid phase at low temperatures (≤10 °C) but converting into gel at temperatures above 20 °C, and formed stable biomembrane at 37 °C. The neomycin impregnated pluronic membrane suppressed the bacterial growth on agar plate by the sustained release of neomycin. The histological images of skin tissue after applying pluronic gel to burn injured area on rabbit confirmed that the pluronic F127 formulation functioned as a matrix to release drug as well as a biomembrane to protect burn injury. Thus, the formulated pluronic F127 may have a potential for the application of local treatment on burn injury.