RESUMO
ADAM metalloprotease-disintegrins mediate cell adhesion, proteolytic processing, and signal transduction. In the present study, the mRNA levels of ADAM9, ADAM10, and ADAM15 were examined in rat brain after kainic acid (KA)-induced status epilepticus. ADAM9 and ADAM10 expression was induced in dentate gyrus of hippocampus. ADAM15 expression remained unchanged. The spatiotemporal expression of ADAM9 and ADAM10 suggests that their regulation after the KA-induced status epilepticus could be related to neuroprotection.
Assuntos
Ácido Aspártico Endopeptidases/genética , Giro Denteado/metabolismo , Desintegrinas/genética , Epilepsia/metabolismo , Proteínas de Membrana/genética , Metaloendopeptidases/genética , Metaloproteases/genética , Estado Epiléptico/metabolismo , Proteínas ADAM , Secretases da Proteína Precursora do Amiloide , Animais , Convulsivantes , Giro Denteado/fisiopatologia , Modelos Animais de Doenças , Endopeptidases , Epilepsia/induzido quimicamente , Epilepsia/fisiopatologia , Agonistas de Aminoácidos Excitatórios/farmacologia , Ácido Caínico , Masculino , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Estado Epiléptico/induzido quimicamente , Estado Epiléptico/fisiopatologia , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/fisiologiaRESUMO
ADAM11 is the prototype member of the predominantly CNS-associated clade of the ADAM metalloprotease-disintegrins that has been implicated in neural adhesion and axon guidance. The present study describes the spatiotemporal expression pattern of the ADAM11 gene in adult and developing mouse, and identifies the cells expressing the gene. In the adult CNS, ADAM11 mRNA was present throughout the forebrain, including different cortical fields and diencephalic nuclei. In brainstem, low to moderate expression was detected in certain midbrain nuclei, while several pontine and medullary nuclei showed a very strong signal. High expression was observed in the cerebellar cortex and spinal cord. In addition, ADAM11 was expressed in ganglia of the peripheral nervous system (PNS), retinae, testes, liver, and at lower levels in epidermal and mucosal epithelia, kidney, and salivary gland. The expression was localized to neurons in all examined CNS and PNS subfields. During pre- and perinatal development, ADAM11 was differentially expressed both in the developing PNS and CNS, as well as in heart, kidney, eyes, and brown fat. The present results suggest a widespread involvement of ADAM11 in neuron-neuron or neuron-glial cell interactions during development as well as in the adult nervous system. They provide novel complementary information to recently accumulated data on CNS integrin gene expression and offer useful clues for further studies of the neural functions of ADAMs and integrins.
