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Objective To study the immune response gene and the mechanism of membranous glomerulonephritis in the mouse. Methods Reproduced and identitied the animal model of MGN in mice ,extracted the total RNA of pathology group and the control group,amplified and verified I-Aβ1 gene through RT-PCR. Then sequenced and analyzed the I-AβI gene from the PCR production. Results The mutation rate of the I-Aβ1 gene was 2. 578‰ in the pathology group, and 0. 286‰ in the control group. It was obviously higher in the pathology group than in the control group(P ＜0.01). Conclusion I-Aβ1 gene rose in mice,may be related to membranous glomerulonephritis.
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Objective To observe changes of plasma coagulation factor Ⅶ(FVII) and antithrombin III( AT-Ⅲ) in patients with acute myacardial infarction(AMI) . Methods The blood samples of 40 patients with AMI and 20 healthy volunteers were obtained through vein at the moment of admission. The plasma activated factors( FVIIag,FVI- la,FVII: C and AT-Ⅲ) were detected with different methods. Results Compared with control group,the plasina levels of FVIIag, FVIIa and the FVII: C in patients with AMI were significantly higher( P < 0.05, P < 0. 01) and especially in FVIIa(P <0.01) ,the AT-Ⅲ activity decreased significantly. Conclusion FVIIa and AT-III play important role in thrombosis of AMI,and the changes of their activities could predict the formation of acute cardiovascular event.
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Objective To study the effects of decoy strategy targeted to NF-KB on the development of trauma-associated liver inflammation in rats. Methods In this study, 108 Wistar rats were randomized into 3 groups: control group, traumatic inflammation group and traumatic inflammation plus decoy ODN group. Rats were sacrificed on 3,6,12,24,48 and 72hrs in each group respectively. Liver functions and structural changes were examined and compared between the groups. DNA binding activity of NF-KB in liver tissue was measured by EMSA. TNF-α and IL-6 gene expressin in liver tissue was assessed by RT-PCR and TNF-α and IL-6 protein level was determined by ELISA. Results The DNA binding activity of NF-kB in liver rose at 3 hours after induction of liver inflammation following trauma and peaked at 12 hours. Correspondingly, both the mRNA and protein levels of TNF-α and IL-6 elevated significantly, as well as the serum alanine aminotransferase level culminating at 24 hours after surgery. Hepatocytes was edematous, degeneration and necrosis, with dramatic destruction of lobular structures. All these changes were significantly inhibited with NF-KB decoy oligodeoxynucleotides. Conclusions Decoy oligodeoxynucleotides specifically inhibit the activity of NF-kB, and the release of pro-inflammatory cytokines, TNF-α and IL-6 release from the liver in response to traumatic inflammation decrease, hence the injury on liver structures and functions were alleviated.
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suitable tumor markers and new targets for gene therapy of cervical carcinoma.
