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1.
Cytometry A ; 77(1): 97-100, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19937951

RESUMO

The flow cytometry data file standard provides the specifications needed to completely describe flow cytometry data sets within the confines of the file containing the experimental data. In 1984, the first Flow Cytometry Standard format for data files was adopted as FCS 1.0. This standard was modified in 1990 as FCS 2.0 and again in 1997 as FCS 3.0. We report here on the next generation flow cytometry standard data file format. FCS 3.1 is a minor revision based on suggested improvements from the community. The unchanged goal of the standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.The FCS 3.1 standard retains the basic FCS file structure and most features of previous versions of the standard. Changes included in FCS 3.1 address potential ambiguities in the previous versions and provide a more robust standard. The major changes include simplified support for international characters and improved support for storing compensation. The major additions are support for preferred display scale, a standardized way of capturing the sample volume, information about originality of the data file, and support for plate and well identification in high throughput, plate based experiments. Please see the normative version of the FCS 3.1 specification in Supporting Information for this manuscript (or at http://www.isac-net.org/ in the Current standards section) for a complete list of changes.


Assuntos
Processamento Eletrônico de Dados/normas , Citometria de Fluxo/normas , Biologia Computacional , Processamento Eletrônico de Dados/métodos , Citometria de Fluxo/métodos , Software/normas
2.
Cytometry A ; 73(10): 926-30, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18752282

RESUMO

A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data.


Assuntos
Sistemas de Gerenciamento de Base de Dados , Citometria de Fluxo/normas , Guias como Assunto , Separação Celular/instrumentação , Separação Celular/métodos , Separação Celular/normas , Citometria de Fluxo/instrumentação , Citometria de Fluxo/métodos , Indicadores e Reagentes/normas , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
3.
Hum Mol Genet ; 14(21): 3219-25, 2005 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-16203743

RESUMO

Chromosome 20q13.2 is amplified in 20-30% of early-stage breast tumors and is associated with poor prognosis. Detailed mapping of the amplified region using molecular cytogenetics, positional cloning and genomic sequencing culminated in a detailed molecular description of the candidate oncogene ZNF217. ZNF217 proteins resemble Kruppel-like transcription factors, localize predominately to the nucleus and associate with proteins involved in transcriptional repression. The findings that ZNF217 can immortalize human mammary epithelial cells and that its amplification is associated with poor prognosis suggest that it may play roles in both early- and late-stage breast cancer. We present evidence that ZNF217 can attenuate apoptotic signals resulting from telomere dysfunction as well as from doxorubicin-induced DNA damage and that silencing ZNF217 with siRNA restores sensitivity to doxorubicin. Moreover, elevated ZNF217 leads to increased phosphorylation of Akt, whereas inhibition of the phosphatidylinositol 3 kinase pathway and Akt phosphorylation decreases ZNF217 protein levels and increases sensitivity to doxorubicin. These results suggest that ZNF217 may promote neoplastic transformation by increasing cell survival during telomeric crisis and may promote later stages of malignancy by increasing cell survival during chemotherapy.


Assuntos
Apoptose/genética , Neoplasias da Mama/genética , Cromossomos Humanos Par 20/genética , Proteínas de Neoplasias/metabolismo , Telômero/fisiologia , Transativadores/metabolismo , Linhagem Celular Tumoral , Dano ao DNA , Primers do DNA , Doxorrubicina , Tratamento Farmacológico , Inativação Gênica , Humanos , Immunoblotting , Proteínas de Neoplasias/genética , Fosfatidilinositol 3-Quinases/metabolismo , Telômero/genética , Transativadores/genética
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